Journal of the American Oil Chemists' Society

, Volume 77, Issue 1, pp 49–53 | Cite as

Phytosterol accumulation in canola, sunflower, and soybean oils: Effects of genetics, planting location, and temperature



To assess the potential of traditional selection breeding to develop varieties with increased phytosterol content, we determined concentrations of those sterols in canola, sunflower, and soybean seed oils produced from breeding lines of diverse genetic backgrounds. Seed oils were extracted and saponified, and the nonsaponifiable fractions were subjected to silylation. The major phytosterols brassicasterol, campesterol, stigmasterol and β-sitosterol, were quantified by capillary gas chromatography with flame-ionization detection. Canola contained approximately twice the amount of total phytosterols (4590–8070 μg g−1) as sunflower (2100–4540 μg g−1) or soybean (2340–4660 μg g−1) oils. Phytosterol composition varied among crops as expected, as well as within a crop. Both genetic background and planting location significantly affected total phytosterol concentrations. Soybean plants were maintained from flower initiation to seed maturity under three temperature regimes in growth chambers to determine the effect of temperature during this period on seed oil phytosterol levels. A 2.5-fold variability in total phytosterol content was measured in these oils (3210–7920 μg g−1). Total phytosterol levels increased with higher temperatures. Composition also changed, with greater percent campesterol and lower percent stigmasterol and β-sitosterol at higher temperatures. In these soybean oils, total phytosterol accumulation was correlated inversely with total tocopherol levels. Owing to the relatively limited variability in phytosterol levels in seed oils produced under field conditions, it is unlikely that a traditional breeding approach would lead to a dramatic increase in phytosterol content or modified phytosterol composition.

Key Words

Cariola oil fatty acid composition genetic modification phytosterols planting location soybean oil sunflower oil temperature tocopherols 


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  1. 1.
    Miettinen, T., P. Puska, H. Gylling, H. Vanhanen, and E. Vartianen, Reduction of Serum Cholesterol with Sitostanol-Ester Margarine in a Midly Hypercholesterolemic Population. N. Engl. J. Med. 333:1308–1312 (1995).CrossRefGoogle Scholar
  2. 2.
    Clark, J., Tocopherols and Sterols from Soybeams, Lipid Technol. 8:111–114 (Sept. 1996).Google Scholar
  3. 3.
    Mounts, T., S. Abidi, and K. Rennick, Effect of Genetic Modification on the Content and Composition of Bioactive Constituents in Soybean Oil, J. Am. Oil Chem. Soc. 73:581–586 (1996).Google Scholar
  4. 4.
    Dolde, D., C. Vlahakis, and J. Hazebroek, Tocopherols in Breeding Lines and Effects of Planting Location, Fatty Acid Composition, and Temperature During Development Ibid. 76:349–355 (1999).Google Scholar
  5. 5.
    Analysis of Oilseeds, Fats and Fatty Foods, edited by J. Rossell and J. Pritchard, Elsevier Applied Science, London, 1990, pp. 315, 317.Google Scholar
  6. 6.
    Gordon, M., and L. Miller, Development of Steryl Ester Analysis for the Detection of Admixtures of Vegetable Oils, J. Am. Oil Chem. Soc. 74:505–510 (1997).Google Scholar
  7. 7.
    The Lipid Handbook, edited by F. Gunstone, J. Harwood, and F. Padley, Chapman & Hall, London, 1994, p. 128.Google Scholar
  8. 8.
    Mozzon, M., F. Bocci, and N. Frega, A Study on the Lipidic Fraction Extracted from High Oleic Sunflower Seeds (Helianthus annuus L.) During the Ripening Process, J. Agric. Food Chem. 46:4198–4202 (1998).CrossRefGoogle Scholar
  9. 9.
    Willemot, C., Sterols in Hardening Winter Wheat, Phytochemistry 19:1071–1073 (1980).CrossRefGoogle Scholar
  10. 10.
    Uemura, M., and S. Yoshida, Involvement of Plasma Membrane Alterations in Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv. Puma), Plant Physiol. 75:818–826 (1984).CrossRefGoogle Scholar
  11. 11.
    Palta, J., B. Whitaker, and L. Weiss, Plasma Membrane Lipids Associated with Genetic Variability in Freezing Tolerance and Cold Acclimation of Solanum Species, Plant Physiol. 103:793–803 (1993).Google Scholar
  12. 12.
    Bartley, I., Changes in Sterol and Phospholipid Composition of Apples During Storage at Low Temperatures and Low Oxygen Concentration, J. Sci. Food Agric. 37:31–36 (1996).CrossRefGoogle Scholar
  13. 13.
    Whitaker, B., Lipid Changes in Microsomes and Crude Plastid Fractions During Storage of Tomato Fruits at Chilling and Nonchilling Temperatures, Phytochemistry 32:265–271 (1993).CrossRefGoogle Scholar
  14. 14.
    Huang, L.-S., and C. Grunwald, Comparative Study of in Vivo Stigmasterol Biosynthesis in Nicotiana tobacum and Hordeum vulgare, Phytochemistry 25:2779–2781 (1986).CrossRefGoogle Scholar
  15. 15.
    Whitaker, B., Lipid Changes in Mature-Green Tomatoes During Ripening, During Chilling, and After Rewarming Subsequent to Chilling, J. Am. Soc. Hort. Sci. 119:994–999 (1994).Google Scholar
  16. 16.
    Whitaker, B., Lipid Changes in Mature-Green Bell Pepper Fruit During Chilling at 2°C and After Transfer to 20°C Subsequent to Chilling, Physiol. Plant. 93:683–688 (1995).CrossRefGoogle Scholar
  17. 17.
    Schuler, I., A. Milon, Y. Nakatani, G. Ourisson, A.-M. Albrecht, P. Benveniste, and M.-A. Hartmann, Differential Effects of Plant Sterols on Water Permeability and on Acyl Chain Ordering of Soybean Phosphatidylcholine Bilayers, Proc. Natl. Acad. Sci. USA 88:6926–6930 (1991).CrossRefGoogle Scholar
  18. 18.
    Guye, M., Sterol Composition in Relation to Chill-Sensitivity in Phaseolus spp, J. Exp. Bot. 39:1091–1096 (1998).Google Scholar
  19. 19.
    Purdy, R., High Oleic Sunflower: Physical and Chemical Characteristics, J. Am. Oil Chem. Soc. 63:1062–1066 (1986).CrossRefGoogle Scholar
  20. 20.
    Fernadez, P., and S. Juan, Study of High Oleic Sunflower Oils, Fatty Acid Composition, Alimentaria 243:63–66 (1993).Google Scholar

Copyright information

© AOCS Press 2000

Authors and Affiliations

  1. 1.Pioneer Hi-Bred International, Inc.Johnston

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