Magic-angle spinning (MAS) 13C nuclear magnetic resonance (NMR) spectroscopy is a convenient method for nondestructive, quantitative characterization of seed oil composition. We describe results for intact hybrid and transformed canola seeds. The MAS 13C NMR technique complements and agrees with gas chromatography results. The spectral resolution approaches that of neat, liquid oils. MAS 13C NMR data allow quantitative analysis of major oil components, including saturates and oleic, linoleic, and linolenic acyl chains. 13C NMR directly and quantitatively elucidates, triglyceride regiochemistry and acyl chain cis-trans isomers that cannot be quickly detected by other methods. MAS 13C NMR can serve as the primary method for development of near-infrared seed oil calibrations. These NMR methods are nondestructive and attractive for plant-breeding programs or other studies (e.g., functional genomics) where loss of seed viability is inconvenient.
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cross-polarization, magic-angle spinning
high-performance liquid chromatography-MS
nuclear Overhauser enhancement
nuclear magnetic resonance
magic-angle spinning; sat, saturated
(1991) Analysis of Oilseeds, Fats and Fatty Foods. (Rossell, J.B., and Pritchard, J.L.R., eds.), Elsevier Science Publishing, New York.
(1997) New Techniques and Applications in Lipid Analysis (McDonald, R.E., and Mossoba, M.M., eds.), AOCS Press, Champaign.
Velasco, L., Fernandez-Martinez, J.M., and De Haro, A. (1997) Determination of the Fatty Acid Composition of the Oil in Intact-Seed Mustard by Near-Infrared Reflectance Spectroscopy, J. Am. Oil Chem. Soc. 74, 1595–1602.
Schaefer, J., and Stejskal, E.O. (1974) Carbon-13 Nuclear Magnetic Resonance Measurement of Oil Composition in Single Viable Soybeans, J. Am. Oil Chem. Soc. 51 210–213.
Schaefer, J., and Stejskal, E.O. (1975) Carbon-13 Nuclear Magnetic Resonance Analysis of Intact Oilseeds, J. Am. Oil Chem. Soc. 52, 366–369.
Rutar, V., Burgar, M., Blinc, R., and Ehrenberg, L. (1977) Carbon-13 NMR Determination of the Oil Composition in Individual Plant Seeds, J. Magnet. Reson. 27, 83–90.
Chen, S., Elofson, R.M., and MacTaggert, J.M. (1979) Carbon-13 Nuclear Magnetic Resonance Studies of Lipids and Starch Digestion in Intact Seeds, J. Agric. Food Chem. 27, 435–438.
Albornoz, F., and Leon, V. (1980) Nordestructive Characterization of Seed Oil Using Carbon-13 FT-NMR, Acta Cient. Venez. 31, 20–23.
Leal, K.Z., Costa, V.E.U., Siedl, P.R., Campos, M.P.A., and Colnago, L. (1981) Immediate Analysis of the Oil Content of Seeds by Carbon-13 Nuclear Magnetic Resonance, Cienc. Cult. (Sao Paulo) 33, 1475–1484.
Yoshida, M., Kano, H., Ishida, N., and Yoshida, T. (1989) Nondestructive Analysis of the Oil Composition of Soybean Seeds by Natural Abundance Carbon-13 Nuclear Magnetic Resonance Spectroscopy, Agric. Biol. Chem. 53, 1395–1400.
Seidl, P.R., and Santos do Nascimento, R. (1990) Analysis of Tropical Oilseeds by Carbon-13 NMR, Spectrosc. Int. J. 8, 183–189.
Gambhir, P.N. (1994) 13C NMR Spectroscopy of Intact Oilseeds—A Rapid Method for Measurement of Fatty Acid Composition, Indian Acad. Sci. Proc. Chem. Sci. 106, 1583–1594.
Ye, C., Sun, B., Qui, J., and Yuan, H. (1986) Magic Angle Spinning NMR of Oilseeds, Kexue Tongbao (foreign Lang. ed.) 31, 16–20.
Rutar, V., Kovac, M., and Lahajnar, G. (1988) Improved NMR Spectra of Liquid Components in Heterogeneous Samples, J. Magnet. Reson. 80, 133–138.
Rutar, V., Kovac, M., and Lahajnar, G. (1989) Nondestructive Study of Liquids in Single Fir Seeds Using Nuclear Magnetic Resonance and Magic Angle Sample Spinning, J. Am. Oil. Chem. Soc. 66, 961–965.
Rutar, V. (1989) Magic Angle Sample Spinning NMR Spectroscopy of Liquids as a Nondestructive Method for Studies of Plant Seeds, J. Agric. Food Chem. 37, 67–70.
Wollenberg, K. (1991) Quantitative Triacylglycerol Analysis of Whole Vegetable Seeds by Proton and Carbon-13 Magic Angle Sample Spinning NMR Spectroscopy, J. Am. Oil Chem. Soc. 68, 391–400.
Ye, C., Guo, Y., and Feng, Y. (1991) MAS NMR Measurement of Oil Content in Oilseed, Chin. Sci. Bull. 36, 597–601.
Keifer, P.A. (1997) High-Resolution NMR Techniques for Solid-Phase Synthesis and Combinatorial Chemistry, Drug Discovery Today 11, 468–478.
(CNMR DB), Version 3.50, Advanced Chemical Development Inc., Toronto, Canada.
Loughman, B.C., and Ratcliffe, R.G. (1984) Nuclar Magnetic Resonance and the Study of Plants, Avd. Plant Nutr. 1, 241–283.
Van Bruggen, P.C., Dachateau, C.S.M.J.E., Mooren, M.M.W., and van Oosten, H.J. (1998) Precision of Low Trans Fatty Acid Level Determination in Refined Oils. Results of a Collaborative Capillary Gas-Liquid Chromatography Study, J. Am. Oil Chem. Soc. 75, 483–488.
O’Keefe, S., Gaskins-Wright, S., Wiley, V., and Chen, I.-C. (1994) Levels of Trans Geometrical Isomers of Essential Fatty Acids in Some Unhydrogenated U.S. Vegetable Oils, J. Food Lipids 1, 165–176.
Shoolery, J.N. (1983) Applications of High Resolution Nuclear Magnetic Resonance to the Study of Lipids, Am. Oil Chem. Soc. Monogr. 220–240.
Gillet, S., and Delpuech, J.J. (1980) Optimum Conditions for Nondestructive Quantitative Analysis by Carbon-13 NMR, J. Magnet. Reson. 38, 433–445.
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Hutton, W.C., Garbow, J.R. & Hayes, T.R. Nondestructive NMR determination of oil composition in transformed canola seeds. Lipids 34, 1339–1346 (1999). https://doi.org/10.1007/s11745-999-0487-0