Skip to main content
Log in

Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid

  • Published:
Lipids

Abstract

Two experiments were conducted. In Experiment 1, 8-wk-old mice were fed control diet or diet supplemented with 0.5% conjugated linoleic acid (CLA) to study the effect of CLA on body composition (CLA: 40.8–41.1% c-9,t-11 isomer, 43.5–44.9% t-10,c-12 isomer). The data for CLA-fed mice vs. controls described parallel but significantly distinct responses for both absolute and relative changes in body fat mass (reduced in CLA-fed mice) and for relative changes in whole body protein and whole body water (both of which were increased in CLA-fed mice). In the CLA-fed mice, the effect on whole body protein appeared to precede the reduction in body fat mass. In Experiment 2, weanling mice were fed control diet or diet supplemented with 0.5% CLA for 4 wk (test group), at which time all mice were fed control diet devoid of added CLA. The test group exhibited significantly reduced body fat and significantly enhanced whole body water relative to controls at the time of diet change. Time trends for changes in relative body composition were described by parallel lines where the test group exhibited significantly less body fat but significantly more whole body protein, whole body water, and whole body ash than controls. Tissue CLA levels declined following the withdrawal of CLA from the diet. In skeletal muscle of mice fed CLA-supplemented diet, the t-10,c-12 isomer was cleared significantly faster than the c-9,t-11 CLA isomer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

ANOVA:

analysis of variance

CLA:

conjugated linoleic acid

References

  1. Ha, Y.L., Grimm, N.K., and Pariza, M.W. (1987) Anticarcinogens from Fried Ground Beef: Heat-Altered Derivatives of Linoleic Acid. Carcinogenesis 8, 1881–1887.

    PubMed  CAS  Google Scholar 

  2. Doyle, E. (1998) Scientific Forum Explores CLA Knowledge, INFORM 9, 69–72.

    Google Scholar 

  3. Park, Y., Albright, K.J., Storkson, J.M., Cook, M.E., and Pariza, M.W. (1997) Effect of Conjugated Linoleic Acid on Body Composition in Mice, Lipids 32, 853–858.

    Article  PubMed  CAS  Google Scholar 

  4. DeLany, F.P., Blohm, F.Y., Truett, A.A., and West, D.B. (1998) Dose Response of Conjugated Linoleic Acid (CLA) on Mouse Body Fat, FASEB J. 12, A504.

    Google Scholar 

  5. Houseknecht, K.L., Vanden Heuvel, J.P., Moya-Camarena, S.Y., Portocarrero, C.P., Peck, L.W., Nickel, K.P., and Belury, M.A. (1998) Dietary Conjugated Linoleic Acid Normalizes Impaired Glucose Tolerance in the Zucker Diabetic Fatty fa/fa rat, Biochem. Biophys. Res. Comm. 244, 678–682.

    Article  PubMed  CAS  Google Scholar 

  6. Sisk, M., Azain, M.J., Hausman, D.B., and Jewell, D.E. (1998) Effect of Conjugated Linoleic Acid on Fat Pad Weights and Cellularity in Sprague-Dawley and Zucker Rats, FASEB J. 12, A536.

    Google Scholar 

  7. Dugan, M.E.R., Aalhus, J.L., Schaefer, A.L., and Kramer, J.K.G. (1997) The Effects of Conjugated Linoleic Acid on Fat to Lean Repartitioning and Feed Conversion in Pigs, Can. J. Anim. Sci. 77, 723–725.

    Article  CAS  Google Scholar 

  8. Cook, M.E., Jerome, D.L., Crenshaw, T.D., Buege, D.R., Pariza, M.W., Albright, K.J., Schmidt, S.P., Scimeca, J.A., Lofgren, P.A., and Hentges, E.J. (1998) Feeding Conjugated Linoleic Acid Improves Feed Efficiency and Reduces Whole Body Fat in Pigs, FASEB J. 12, A836.

    Google Scholar 

  9. Thiel, R.L., Sparks, J.C., Wiegand, B.R., Parrish, F.C., Jr., and Ewan, R.C. (1998) Conjugated Linoleic Acid Improves Performance and Body Composition in Swine, Midwest Animal Science Meetings 1998 Abstracts, p. 61, Midwestern Section, American Society of Animal Science, Savoy, IL.

    Google Scholar 

  10. Dunshea, F.R., Ostrowska, E., Muralitharan, M., Cross, R., Bauman, D.E., Pariza, M.W., and Skaric, C. (1998) Dietary Conjugated Linoleic Acid Decreases Back Fat in Finisher Gilts, J. Animal Sci. 76 (Suppl. 1), 131.

    Google Scholar 

  11. Cohen, L.A., Choi, K., and Wang, C.-X. (1988) Influence of Dietary Fat, Caloric Restriction, and Voluntary Exercise on N-Nitrosomethylurea-Induced Mammary Tumorigenesis in Rats, Cancer Res. 48, 4276–4283.

    PubMed  CAS  Google Scholar 

  12. Ha, Y.L., Storkson, J., and Pariza, M.W. (1990) Inhibition of Benzo(α)pyrene-Induced Mouse Forestomach Neoplasia by Conjugated Dienoic Derivatives of Linoleic Acid, Cancer Res. 50, 1097–1101.

    PubMed  CAS  Google Scholar 

  13. Chin, S.F., Liu, W., Storkson, J.M., Ha, Y.L., and Pariza, M.W. (1992) Dietary Sources of Conjugated Dienoic Isomers of Linoleic Acid, a Newly Recognized Class of Anticarcinogens, J. Food Comp. Anal. 5, 185–197.

    Article  CAS  Google Scholar 

  14. Sehat, N., Yurawecz, M.P., Roach, J.A.G., Mossoba, M.M., Kramer, J.K.G., and Ku, Y. (1998) Silver-Ion High-Performance Liquid Chromatographic Separation and Identification of Conjugated Linoleic Acid Isomers, Lipids 33, 217–221.

    Article  PubMed  CAS  Google Scholar 

  15. Helrich, K. (1990) Official Methods of Analysis, 15th edn., pp. 935–937. Association of Official Analytical Chemists Inc., Arlington.

    Google Scholar 

  16. Forbes, G.B. (1996) Body Composition, in Present Knowledge in Nutrition (Ziegler, E.E., and Filer, L.J., Jr., eds.), pp. 7–18, ILSI Press, Washington, DC.

    Google Scholar 

  17. Satory, D., and Smith, S.B. (1998) Conjugated Linoleic Acid Inhibits Proliferation, but Stimulates Differentiation of 3T3-L1 Preadipocytes, FASEB J. 12, A552.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Michael W. Pariza.

About this article

Cite this article

Park, Y., Albright, K.J., Storkson, J.M. et al. Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid. Lipids 34, 243–248 (1999). https://doi.org/10.1007/s11745-999-0359-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11745-999-0359-7

Keywords

Navigation