Skip to main content
SpringerLink
Log in

Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice

  • Article
  • Published:
Lipids

Abstract

We investigated the effects of conjugated linoleic acid (CLA) preparations, which were enriched for the cis-9,trans-11 CLA isomer or the trans-10,cis-12 CLA isomer, on body composition in mice. Body composition changes (reduced body fat, enhanced body water, enhanced body protein, and enhanced body ash) were associated with feeding the trans-10,cis-12 CLA isomer. In cultured 3T3-L1 adipocytes, the trans-10,cis-12 isomer reduced lipoprotein lipase activity, intracellular triacylglycerol and glycerol, and enhanced glycerol release into the medium. By contrast, the cis-9,trans-11 and trans-9,trans-11 CLA isomers did not affect these biochemical activities. We conclude that CLA-associated body composition change results from feeding the trans-10,cis-12 isomer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CLA:

conjugated linoleic acid

DMEM:

Dulbecco's modified Eagle's medium

FBS:

fetal bovine serum

LPL:

lipoprotein lipase

References

  1. Ha, Y.L., Grimm, N.K., and Pariza, M.W. (1987) Anticarcinogens from Fried Ground Beef: Heat-Altered Derivatives of Linoleic Acid, Carcinogenesis 8, 1881–1887.

    PubMed  CAS  Google Scholar 

  2. Doyle, E. (1998) Scientific Forum Explores CLA Knowledge, INFORM 9, 69–72.

    Google Scholar 

  3. Park, Y., Albright, K.J., Storkson, J.M., Cook, M.E., and Pariza, M.W. (1997) Effect of Conjugated Linoleic Acid on Body Composition in Mice, Lipids 32, 853–858.

    Article  PubMed  CAS  Google Scholar 

  4. West, D.B., DeLany, J.P., Camet, P.M., Blohm, F., Truett, A.A., and Scimeca, J. (1998) Effects of Conjugated Linoleic Acid on Body Fat and Energy Metabolism in the Mouse, Am. J. Physiol.-Reg. I. 44, R667-R672.

    Google Scholar 

  5. Houseknecht, K.L., Vanden Heuvel, J.P., Moya-Camarena, S.Y., Portocarrero, C.P., Peck, L.W., Nickel, K.P., and Belury, M.A. (1998) Dietary Conjugated Linoleic Acid Normalizes Impaired Glucose Tolerance in the Zucker Diabetic Fatty fa/fa Rat, Biochem. Biophys. Res. Comm. 244, 678–682.

    Article  PubMed  CAS  Google Scholar 

  6. Sisk, M., Azain, M.J., Hausman, D.B., and Jewell, D.E. (1998) Effect of Conjugated Linoleic Acid on Fat Pad Weights and Cellularity in Sprague-Dawley and Zucker Rats, FASEB J. 12, A536.

    Google Scholar 

  7. Dugan, M.E.R., Aalhus, J.L., Schaefer, A.L., and Kramer, J.K.G. (1997) The Effects of Conjugated Linoleic Acid on Fat to Lean Repartitioning and Feed Conversion in Pigs, Can. J. Anim. Sci. 77, 723–725.

    Article  CAS  Google Scholar 

  8. Cook, M.E., Jerome, D.L., Crenshaw, T.D., Buege, D.R., Pariza, M.W., Albright, K.J., Schmidt, S.P., Scimeca, J.A., Lofgren, P.A., and Hentges, E.J. (1998) Feeding Conjugated Linoleic Acid Improves Feed Efficiency and Reduces Whole Body Fat in Pigs, FASEB J. 12, A836.

    Google Scholar 

  9. Thiel, R.L., Sparks, J.C., Wiegand, B.R., Parrish, F.C., Jr., and Ewan, R.C. (1998) Conjugated Linoleic Acid Improves Performance and Body Composition in Swine, Midwest Animal Science Meetings 1998 Abstracts, Abstract #127, p. 61, Midwestern Section, American Society of Animal Science, Savoy, IL.

    Google Scholar 

  10. Dunshea, F.R., Ostrowska, E., Muralitharan, M., Cross, R., Bauman, D.E., Pariza, M.W., and Skarie, C. (1998) Dietary Conjugated Linoleic Acid Decreases Back Fat in Finisher Gilts, J. Animal Sci. 76 (Suppl. 1), 131.

    Google Scholar 

  11. Ha, Y.L., Storkson, J., and Pariza, M.W. (1990) Inhibition of Benzo(α)pyrene-Induced Mouse Forestomach Neoplasia by Conjugated Dienoic Derivatives of Linoleic Acid. Cancer Res. 50, 1097–1101.

    PubMed  CAS  Google Scholar 

  12. Chin, S.F., Liu, W., Storkson, J.M., Ha, Y.L., and Pariza, M.W. (1992) Dietary Sources of Conjugated Dienoic Isomers of Linoleic Acid, a Newly Recognized Class of Anticarcinogens, J. Food Comp. Anal. 5, 185–197.

    Article  CAS  Google Scholar 

  13. Sehat, N., Yurawecz, M.P., Roach, J.A.G., Mossoba, M.M., Kramer, J.K.G., and Ku, Y. (1998) Silver-Ion High-Performance Liquid Chromatographic Separation and Identification of Conjugated Linoleic Acid Isomers, Lipids 33, 217–221.

    Article  PubMed  CAS  Google Scholar 

  14. Lee, K.N., Pariza, M.W., and Ntambi, J.M. (1998) Conjugated Linoleic Acid Decreases Hepatic Stearoyl-CoA Desaturase mRNA Expression. Biochem. Biophys. Res. Comm. 248, 817–821.

    Article  PubMed  CAS  Google Scholar 

  15. Scholfield, C.R. (1970) A Simple Method for Preparation of Methyl trans-10,cis-12 Octadecadienoate, J. Am. Oil Chem. Soc. 47, 303.

    CAS  Google Scholar 

  16. Helrich, K. (1990) Official Methods of Analysis, 15th edn., pp. 935–937, Association of Official Analytical Chemists Inc., Arlington.

    Google Scholar 

  17. Frost, S.C., and Lane, M.D. (1985) Evidence for the Involvement of Vicinal Sulfhydryl Groups in Insulin-Activated Hexose Transport by 3T3-L1 Adipocytes, J. Biol. Chem. 260, 2646–2652.

    PubMed  CAS  Google Scholar 

  18. Nilsson-Ehle, P., and Schotz, M.C. (1976) A Stable, Radioactive Substrate Emulsion for Assay of Lipoprotein Lipase, J. Lipid Res. 17, 536–541.

    PubMed  CAS  Google Scholar 

  19. Snedecor, G.W., and Cochran, W.G. (1989) Statistical Methods, 8TH edn., pp. 238–240, Iowa State University Press, Ames.

    Google Scholar 

  20. Sebedio, J.L., Juaneda, P., Dobson, G., Ramilison, I., Martin, J.C., Chardigny, J.M., and Christie, W.W. (1997) Metabolites of Conjugated Isomers of Linoleic Acid (CLA) in the Rat, Biochim. Biophys. Acta 1345, 5–10.

    PubMed  CAS  Google Scholar 

  21. Pariza, M.W., Park, Y., Albright, K.J., Liu, W., Storkson, J.M., and Cook, M.E. (1998) Synthesis and Biological Activity of Conjugated Eicosadienoic Acid, Abstracts of the 89th American Oil Chemists' Society Annual Meeting, p. 21. AOCS, Champaign.

    Google Scholar 

  22. Lee, K.N., Kritchevsky, D., and Pariza, M.W. (1994) Conjugated Linoleic Acid and Atherosclerosis in Rabbits, Atherosclerosis 108, 19–25.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Food Research Institute, Department of Food Microbiology and Toxicology, University of Wisconsin-Madison, 1925 Willow Dr., 53706, Madison, Wisconsin

    Yeonhwa Park, Jayne M. Storkson, Karen J. Albright, Wei Liu & Michael W. Pariza

Authors
  1. Yeonhwa Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jayne M. Storkson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Karen J. Albright
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wei Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Michael W. Pariza
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michael W. Pariza.

About this article

Cite this article

Park, Y., Storkson, J.M., Albright, K.J. et al. Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids 34, 235–241 (1999). https://doi.org/10.1007/s11745-999-0358-8

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11745-999-0358-8

Keywords

Search

Navigation