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Vitamin E reduces cholesterol esterification and uptake of acetylated low density lipoprotein in macrophages

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Lipids

Abstract

The effects of vitamin E on cholesteryl ester (CE) metabolism in 1774 cells were examined. Pretreatment of 1774 cells with vitamin E at concentrations above 50 μM significantly decreased acetylated low density lipoprotein (LDL)-induced incorporation of [14C]oleate into CF in cells in a dose-dependent manner. This was partly due to vitamin E Also significantly inhibiting the uptake of [3H]CE-labeled acetylated LDL by 1774 cells. A trend existed toward suppression of acyl-CoA:cholesterol acyltransferase (ACAT) activity in the cell lysate at high vitamin E concentration, but there was no effect on hydrolysis of CE. These data indicate that vitamin E reduces the uptake of modified LDL and suppresses ACAT activity, resulting in less cholesterol esterification in macrophages; a novel mechanism underlying the antiatherogenic properties of vitamin E.

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Abbreviations

ACAT:

acyl-CoA:cholesterol acyltransferase

ANOVA:

analysis of variance

CE:

cholesteryl ester

CHD:

coronary heart disease

DMEM:

Dulbecco's modified Fagle's medium

FBS:

Tetal bovine serum

LDL:

low density lipoprotein

LPDS:

lipoprotein-deficient serum

PBS:

Phosphate buffer saline

PKC:

protein kinase C

PMSF:

phenylmethylsulfonyl fluoride

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. First Department of Internal Medicine, National Defense Medical College, Commercial Road, 359, Tokorozawa Saitama, Japan

    Hideki Shige, Toshitsugu Ishikawa, Michio Suzukawa, Masato Nishiwaki, Takeshi Yamashita, Kei Nakajima, Toshimitsu Ito, Kenji Higashi, Makoto Ayaori, Atsushi Yonemura & Haruo Nakamura

  2. Cardiovascular Nutrition Laboratory, Baker Medical Research Institute, 3181, Prahran, Victoria, Australia

    Paul Nestel

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  1. Hideki Shige
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  2. Toshitsugu Ishikawa
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Correspondence to Hideki Shige.

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Shige, H., Ishikawa, T., Suzukawa, M. et al. Vitamin E reduces cholesterol esterification and uptake of acetylated low density lipoprotein in macrophages. Lipids 33, 1169–1175 (1998). https://doi.org/10.1007/s11745-998-0320-9

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  • DOI: https://doi.org/10.1007/s11745-998-0320-9

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