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Effects of dietary methionine and cystine on lipid metabolism in hepatoma-bearing rats with hyperlipidemia

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Lipids

Abstract

Abnormal lipid metabolism and its restoration by dietary methionine (Met) and cystine (Cys) were studied in Donryu rats subcutaneously implanted with an ascites hepatoma cell line of AH109A. The hepatoma-bearing rats exhibited byperlipidemia characterized by rises in serum triglyceride and cholesterol levels. Decreased lipoprotein lipase (LPL) activities in epididymal adipose tissue, cardiac muscle, and gastrocnemius as well as increased fatty acid mobilization from adipose tissue were considered to be responsible for the hepatoma-induced hypertriglyceridemia, while increased hepatic cholesterogenesis and decreased steroid excretion into feces were thought to be responsible for the hepatoma-induced hypercholesterolemia. Dietary-supplemented Met or Cys reduced the AH109A-induced hypertriglyceridemia with suppression of fatty acid synthesis in the host liver. Met restored the fall of LPL activities, while Cys did not. Dietary Met or Cys also reduced the hypercholesterolemia with restoration of decreased bile acid excretion into feces. These results suggest that dietary Met or Cys is hypolipidemic in the hepatoma-bearing rats with slight differences in their modes of action.

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Abbreviations

20C:

20% casein diet

Ch:

cholesterol

Cys:

cystine

FA:

fatty acid

H:

hepatoma-bearing group

HDL:

high density lipoprotein

HSL:

hormone-sensitive lipase

HTGL:

hepatic triglyceride lipase

LDL:

low density lipoprotein

Met:

methionine

N:

normal group

LPL:

lipoprotein lipase

NEFA:

nonesterified fatty acid

PBS(-):

Ca2+, Mg2+-free.phosphate-buffered saline

PL:

phospholipid

T-Ch:

total cholesterol

TG:

triglyceride

TNF:

tumor necrosis factor-α

VLDL:

very low density lipoprotein

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Authors and Affiliations

  1. Department of Applied Biological Science, Tokyo Noko University, Saiwai-cho 3-5-8, Fuchu, 183-8509, Tokyo, Japan

    Masashi Kawasaki, Ryuhei Funabiki & Kazumi Yagasaki

Authors
  1. Masashi Kawasaki
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  2. Ryuhei Funabiki
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  3. Kazumi Yagasaki
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Correspondence to Kazumi Yagasaki.

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Kawasaki, M., Funabiki, R. & Yagasaki, K. Effects of dietary methionine and cystine on lipid metabolism in hepatoma-bearing rats with hyperlipidemia. Lipids 33, 905–911 (1998). https://doi.org/10.1007/s11745-998-0287-6

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  • DOI: https://doi.org/10.1007/s11745-998-0287-6

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