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Changes in tissue polyunsaturated fatty acids with age, in spontaneously hypertensive rats

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Lipids

Abstract

The relationship between the biosynthesis of long-chain fatty acids and their distribution in the key organs of hypertension is of considerable interest because of their role in the production of vasoactive eicosanoids and their effects on membrane properties. The present study analyzed the fatty acid compositions of the total lipids in the kidney, aorta, heart, and hepatocytes of 1-, 3-, and 6-mon-old spontaneously hypertensive rats (SHR) and their normotensive controls, Wistar Kyoto rats (WKY) by capillary gas chromatography. The major changes concerned the polyunsaturated fatty acids (PUFA). The percentage of arachidonic acid (AA) was significantly greater in the 1-mon-old SHR kidney than in the WKY kidney, but it was lower at 3 and 6 mon. The percentage of eicosapentaenoic acid was very low in the SHR kidney. The results for the aorta were similar, with marked decreases in 18:2n−6 and 18:3n−3 in SHR aged 1 and 6 mon. Despite a higher proportion of 18:2n−6 and AA at 6 mon, there was no major change in the SHR heart lipids. The fatty acid spectrum in the liver provides additional evidence for the previously reported inhibition of desaturase activities in SHR. Thus, this study shows that the PUFA composition is modified differently in different tissues in SHR, and this may be related to the pathogenesis of hypertension in these animals.

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Abbreviations

AA:

arachidonic acid

DGLA:

dihomo-γ-linolenic acid

DHA:

docosahexaenoic acid

DPA:

docosapentaenoic acid

EPA:

ercosapentaenoic acid

LA:

linoleic acid

PL:

phospholipids

PUFA:

polyunsaturated fatty acids

SFA:

saturated fatty acids

SHR:

spontaneously hypertensive rat

WKY:

Wistar Kyoto

References

  1. Mills, D.E., and Huang, Y.S. (1992) Metabolism of n−6 and n−3 Polyunsaturated Fatty Acids in Normotensive and Hypertensive Rats, in Essential Fatty Acids and Eicosanoids (Sinclair, A., and Gibson, R., eds.) pp. 345–348, American Oil Chemists' Society, Champaign.

    Google Scholar 

  2. Millanvoye-Van Brussel, E., Simon, J., and Freyss-Beguin, M. (1994) Altered Phospholipid Fatty Acid Content and Metabolism in Heart Cell Cultures from Newborn Spontaneously Hypertensive Rats. Am. J. Hypertens. 7, 953–959.

    PubMed  CAS  Google Scholar 

  3. Das, U.N. (1995) Essential Fatty Acid Metabolism in Patients with Essential Hypertension, Diabetes Mellitus and Coronary Heart Disease, Prostaglandins Leukotrienes Essent. Fatty Acids 52, 387–391.

    Article  CAS  Google Scholar 

  4. Mtabaji, J.P., Manku, M.S., and Horrobin, D.F. (1993) Abnormalities in Dihomo-γ-linolenic Acid Release in the Pathogenesis of Hypertension, Am. J. Hypertens. 6, 458–462.

    PubMed  CAS  Google Scholar 

  5. Okamoto, H., Kawaguchi, H., Yasuda, H., Minami, M., and Saito, H. (1986) Changes in Renal Microsomal Lipids in Spontaneously Hypertensive Rats. Prog. Lipid Res. 25, 317–320.

    Article  CAS  Google Scholar 

  6. Watanabe, Y., Huang, Y.S., Simmons, V.A., and Horrobin, D.F. (1989) The Effect of Dietary n−6 and n−3 Polyunsaturated Fatty Acids on Blood Pressure and Tissue Fatty Acid Composition in Spontaneously Hypertensive Rats, Lipids 24, 638–644.

    PubMed  CAS  Google Scholar 

  7. Narce, M., Asdrubal, P., Delachambre, M-C., Vericel, E., Lagarde, M., and Poisson, J.-P. (1994) Age-Related Changes in Linoleic Acid Bioconversion by Isolated Hepatocytes from Spontaneously Hypertensive and Normotensive Rats, Mol. Cell. Biochem. 141, 9–13.

    Article  PubMed  CAS  Google Scholar 

  8. Narce, M., Asdrubal, P., Delachambre, M-C., Gresti, J., and Poisson, J.-P. (1995) Influence of Spontaneous Hypertension on n−3 Delta-6-Desaturase Activity and Fatty Acid Composition of Rat Hepatocytes, Mol. Cell. Biochem. 152, 7–12.

    PubMed  CAS  Google Scholar 

  9. Narce, M., and Poisson, J.-P. (1995) Age-Related Depletion of Linoleic Acid Desaturation in Liver Microsomes from Young Spontaneously Hypertensive Rats. Prostaglandins Leukotrienes Essent. Fatty Acids 53, 59–63.

    Article  CAS  Google Scholar 

  10. Naftilan, A.J., Dzau, V.J., and Loscalzo, J. (1986) Preliminary Observations on Abnormalities of Membrane Structure and Function in Essential Hypertension, Hypertension Suppl. II, 8, 174–179.

    Google Scholar 

  11. Dominiczak, A.F., McLaren, Y., Kusel, J.R., Ball, D.L., Goodfriend, L., Bohr, D.F., and Reid, J.L. (1993) Lateral Diffusion and Fatty Acid Composition in Vascular Smooth Muscle Membrane from Stroke-Prone Spontaneously Hypertensive Rats, Am. J. Hypertens. 6, 1003–1008.

    PubMed  CAS  Google Scholar 

  12. Stubbs, C.D., and Smith, A.D. (1984) The Modification of Mammalian Membrane Polyunsaturated Fatty Acid Composition in Relation to Membrane Fluidity and Function, Biochim. Biophys. Acta 779, 89–137.

    PubMed  CAS  Google Scholar 

  13. Ollerenshaw, J.D., Heagerty, A.M., Bing, R.F., and Swales, J.D. (1987) Abnormalities of Erythrocyte Membrane Fatty Acid Composition in Human Essential Hypertension. J. Hum. Hypertens. 1, 9–12.

    PubMed  CAS  Google Scholar 

  14. Sardesai, V.M. (1992) Nutritional Role of Polyunsaturated Fatty Acids, J. Nutr. Biochem. 3, 154–166.

    Article  CAS  Google Scholar 

  15. Oates, J.A., Fitzgerald, G.A., Branch, R.A., Jackson, E.K., Knapp, H.R., and Roberts, L.J. (1988) Clinical Implications of Prostaglandin and Thromboxane A2 Formation. (first of two parts), N. Engl. J. Med. 319, 689–698.

    Article  PubMed  CAS  Google Scholar 

  16. Oates, J.A., Fitzgerald, G.A., Branch, R.A., Jackson, E.K., Knapp, H.R., and Roberts, L.J. (1988) Clinical Implications of Prostaglandin and Thromboxane A2 Formation. (second of two parts). N. Engl. J. Med. 319, 761–767.

    Article  PubMed  CAS  Google Scholar 

  17. Kirtland, S.J. (1988) Prostaglandin E1: A Review, Prostaglandins Leukotrienes Essent. Fatty Acids, 165–174.

  18. Horrobin, D.F. (1988) Prostaglandin E1: Physiological Significance and Clinical Use, Wiener. Klin Wochenschr. 100, 471–477.

    CAS  Google Scholar 

  19. Fogari, R., and Zoppi, A. (1989) Heart and Hypertension, Am. J. Hypertens. 2, S16-S23.

    Google Scholar 

  20. Markow, K.M., and Atrokhov, V.V. (1985) Characteristics of Early Disorders of Fatty Acid Composition of Kidney Lipids in Spontaneous (hereditary) Arterial Hypertension, Kardiologiya 25, 78–81.

    Google Scholar 

  21. Kawaguchi, H., and Yasuda, H. (1986) Increased Phospholipase A2 Activity in the Kidney of Spontaneously Hypertensive Rats, J. Hypertens. 4, S387-S389.

    Article  CAS  Google Scholar 

  22. Okamoto, H., Kawaguchi, H., Minami, M., Saito, H., and Yasuda, H. (1989) Lipid Alterations in Renal Membrane of Stroke-Prone Spontaneously Hypertensive Rats, Hypertension 13, 456–462.

    PubMed  CAS  Google Scholar 

  23. Limas, C., Goldman, P., and Limas, C.J. (1981) Aortic Phospholipid pholipid Deacylation-Reacylation Cycle in Spontaneously Hypertensive Rats, Am. J. Physiol. 240, H33-H38.

    PubMed  CAS  Google Scholar 

  24. Okumura, K., Kondo, J., Shirai, Y., Muramatsu, M., Yamada, Y., Hashimoto, H., and Ito, T. (1990), 1,2 Diacyl Glycerol Content in Thoracic Aorta of Spontaneously Hypertensive Rats, Hypertension 16, 43–48.

    PubMed  CAS  Google Scholar 

  25. Seglen, P.O., (1973) Preparation of Liver Cells in Enzymatic Requirements for Tissue Dispersion, Exp. Cell. Res. 82, 391–398.

    Article  PubMed  CAS  Google Scholar 

  26. Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animals Tissues, J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  27. Slover, H.T., and Lanza, E. (1979) Quantitative Analysis of Food Fatty Acids by Capillary Gas Chromatography, J. Am. Oil Chem. Soc. 56, 933–943.

    CAS  Google Scholar 

  28. Huang, Y.-S., Cantrill, R.C., DeMarco, A., Campbell, L., Lin, X., Horrobin, D.F., and Mills, D.E. (1994) Differences in the Metabolism of 18:2n−6 and 18:3n−6 by the Liver and Kidney May Explain the Anti-Hypertensive Effect of 18:3n−6, Biochem. Med. Metab. Biol. 51, 27–34.

    Article  PubMed  CAS  Google Scholar 

  29. Murthy, M., Huang, Y.-S., Poisson, J.-P., and Mills, D.E. (1994) Effect of Salt-Loading on Acyltransferase Activity in Genetically Normotensive and Hypertensive Rats, Nutr. Metab. Cardiov. Dis. 4, 43–45.

    CAS  Google Scholar 

  30. Horrobin, D.F., Bryant, R.W., Low, C.F., Pupillo, M.B., and Vanderhoek, J.Y. (1982) Leukotrienes and Other Lipoxygenase Products (Samuelsson, B., and Paoletti, R., eds.), Raven Press, New York, pp. 341–353.

    Google Scholar 

  31. Martineau, A., Robillard, M., Falardeau, P. (1984) Defective Synthesis of Vasodilator Prostaglandins in the Spontaneously Hypertensive Rat, Hypertension 6, I161-I165.

    PubMed  CAS  Google Scholar 

  32. Falardeau, P., Robillard, M., and Martineau, A. (1985) Urinary Levels of 2,3-Dinor-6-oxo-PGF1 alpha: A Reliable Index of the Production of PGI2 in the Spontaneously Hypertensive Rat, Prostaglandins 29, 621–628.

    Article  PubMed  CAS  Google Scholar 

  33. Hui, R., Robillard, M., and Falardeau, P. (1992) Inhibition of Vasopressin-Induced Formation of Diradylglycerols in Vascular Smooth Muscle Cells by Incorporation of Eicosapentaenoic Acid in Membrane Phospholipids, J. Hypertens. 10, 1145–1153.

    Article  PubMed  CAS  Google Scholar 

  34. Mtabaji, J.P., Manku, M.S., and Horrobin, D.F. (1988) Release of Fatty Acids by Perfused Vascular Tissue in Normotensive and Hypertensive Rats, Hypertension 12, 39–45.

    PubMed  CAS  Google Scholar 

  35. Limas, C.J., and Limas, C. (1977) Vascular Prostaglandin Synthesis in the Spontaneously Hypertensive Rat, Am. J. Physiol. 233, H493-H499.

    PubMed  CAS  Google Scholar 

  36. Millanvoye-Van Brussel, E. Simon, J., Devynck, M.A., and Freyss-Beguin, M. (1992) Impairment of Lipid Metabolism in Heart Cell Cultures from Newborn Spontaneously Hypertensive Rat, in Genetic Hypertension. Colloque INSERM (Sassard, J., ed.) Vol. 218, 429-431.

  37. Frieberg, P., Nordlander, M., Lundin, S., and Folkow, B. (1985) Effect of Ageing on Cardiac Performance and Coronary Flow in Spontaneously Hypertensive and Normotensive Rats, Acta Physiol. Scand., 125, 1–11.

    Article  Google Scholar 

  38. Vork, M.M., Trigault, N., Snoeckx, L., Glatz, J.F., and Van der Vusse, G.J. (1992) Heterogeneous Distribution of Fatty Acid-Binding Protein in the Heart of Wistar Kyoto and Spontaneously Hypertensive Rats, J. Mol. Cell. Cardiol. 24, 317–321.

    Article  PubMed  CAS  Google Scholar 

  39. Holman, R.T., (1986) Control of Polyunsaturated Acids in Tissue Lipids, J. Am. Coll. Nutr. 5, 183–211.

    PubMed  CAS  Google Scholar 

  40. Engler, M.M., Karanian, J.W., and Salem, N., Jr. (1991) Influence of Dietary Polyunsaturated Fatty Acids on Aortic and Platelet Fatty Acid Composition in the Rat. Nutr. Res. 11, 753–763.

    Article  CAS  Google Scholar 

  41. Fang, X., Kaduce, T.L., Weintraub, N.L., Van Rollins, M., and Spector, A.A. (1996) Functional Implications of a Newly Characterized Pathway of 11,12-Epoxyeicosatrienoic Acid Metabolism in Arterial Smooth Muscle, Circ. Res. 79, 784–793.

    PubMed  CAS  Google Scholar 

  42. Moffat, M.P., Ward, C.A., Bend, J.R., Mock, T., Farhanggkhoee, P., and Karmazyn, M. (1993) Effects of Epoxyeicosatrienoic Acids on Isolated Hearts and Ventricular Myocytes. Am. J. Physiol. 264, H1154-H1160.

    PubMed  CAS  Google Scholar 

  43. Imaoka, S., Wedlund, P.J., Ogawa, H., Kimura, S., Gonzales, F.J., and Kim, H.Y. (1993) Identification of CYP2C23 Expressed in Rat Kidney as an Arachidonic Acid Epoxygenase, J. Pharmacol. Exp. Ther. 267, 1012–1016.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Unité de Nutrition Cellulaire et Métabolique, Faculté des Sciences Mirande, Université de Bourgogne, BP 400, 21011, Dijon Cedex, France

    Marie-Claire Delachambre, Michel Narce, Philippe Asdrubal & Jean-Pierre Poisson

Authors
  1. Marie-Claire Delachambre
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  2. Michel Narce
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  3. Philippe Asdrubal
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  4. Jean-Pierre Poisson
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Correspondence to Michel Narce.

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Delachambre, MC., Narce, M., Asdrubal, P. et al. Changes in tissue polyunsaturated fatty acids with age, in spontaneously hypertensive rats. Lipids 33, 795–801 (1998). https://doi.org/10.1007/s11745-998-0272-0

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  • DOI: https://doi.org/10.1007/s11745-998-0272-0

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