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Microsequencing of bovine cerebrospinal fluid apolipoproteins: Identification of bovine apolipoprotein E

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Lipids

Abstract

In studies of bovine plasma lipoproteins, apolipoprotein E (apoE) was not found associated with α-lipoproteins isolated over a broad range of densities. However, studies of cerebrospinal fluid (CSF) lipoproteins from other mammals have shown that apoE is a major apolipoprotein associated with high density lipoprotein, a fact that prompted us to determine if this were also the case in bovine CSF. CSF samples were obtained from animals with a surgically implanted catheter. Most analyzed samples were obtained from cows at various stages of the postpartum period; however, a few samples also were obtained at term or during pregnancy. Analyses of isolated ultracentrifugal fractions by polyacrylamide gel electrophoresis revealed the presence of two apo, with the expected molecular weights for apoE and apoA-I. By using both matrix-assisted laser desorption mass spectrometry and microsequencing techniques, we demonstrated that these apo are indeed apoE and apoA-I.

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Abbreviations

apo:

apolipoprotein

CSF:

cerebrospinal fluid

HDL:

high density lipoprotein

LDL:

low density lipoprotein

MALD-MS:

matrix assisted laser desorption mass spectrometry

PAGE:

polyacrylamide gel electrophoresis

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Author information

Authors and Affiliations

  1. Molecular Biology Institute, University of California at Los Angeles, 405 Hilgard Ave., 90095, Los Angeles, California

    Donald L. Puppione

  2. The Salk Institute, 92037, La Jolla, California

    Wolfgang H. Fischer & Minkyu Park

  3. Animal Reproduction Laboratory, Texas A&M University Agricultural Research Station, 78102, Beeville, Texas

    Oladele S. Gazal & Gary L. Williams

Authors
  1. Donald L. Puppione
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  2. Wolfgang H. Fischer
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  3. Minkyu Park
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  4. Oladele S. Gazal
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  5. Gary L. Williams
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Correspondence to Donald L. Puppione.

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Puppione, D.L., Fischer, W.H., Park, M. et al. Microsequencing of bovine cerebrospinal fluid apolipoproteins: Identification of bovine apolipoprotein E. Lipids 33, 781–786 (1998). https://doi.org/10.1007/s11745-998-0270-2

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  • DOI: https://doi.org/10.1007/s11745-998-0270-2

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