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Cyclic fatty acid monomers from dietary heated fats affect rat liver enzyme activity

  • Published:
Lipids

Abstract

This study was conducted to investigate the effects of dietary cyclic fatty acid monomers (CFAM), contained in heated fat from a commercial deep-fat frying operation, on rat liver enzyme activity. A partially hydrogenated soybean oil (PHSBO) used 7 d (7-DH) for frying foodstuffs, or 0.15% methylated CFAM diets was fed to male weanling rats in comparison to a control group fed a nonheated PHSBO (NH) diet in a 10-wk experiment. All diets were isocaloric with 15% fat. Animals fed either CFAM or 7-DH diets showed increased hepatic content of cytochrome (cyt.) b5 and P450 and increased activity of (E.C. 1.6.2.4) NADPH-cyt. P450 reductase in comparison to the control rats. In addition, the activities of (E.C. 2.3.1.21) carnitine palmitoyltransferase-l and (E.C. 1.1.1.42) isocitrate dehydrogenase were significantly decreased when compared to that of rats fed the NH diet. A significantly depressed activity of (E.C. 1.1.1.49) glucose 6-phosphate dehydrogenase was also observed for these animals compared to the control rats fed NH diet. Moreover, liver and microsomal proteins were significantly increased when CFAM or 7-DH diets were fed to animals in comparison to controls while liver glycogen was decreased significantly in experimental groups of rats. The results obtained in this study indicate that the CFAM in the diet from either synthetic sources or used fats increase the activity of liver enzyme systems that detoxify them.

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Abbreviations

CFAM:

cyclic fatty acid monomers

CFAME:

methylated CFAM

Cpt-I:

carnitine palmitoyltransferase-I

cyt:

cytochrome

7-DH:

7 d for frying foodstuffs

ICDH:

isocitrate dehydrogenase

NH:

nonheated

PHSBO:

partially hydrogenated soybean oil

TCA:

tricarboxylic acid

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Authors and Affiliations

  1. Faculté des Sciences, Département de Biochimie/Nutrition, Université du Bénin, Lomé, Togo

    Courdjo Lamboni

  2. Station de Recherches sur la Qualité des Aliments de l'Homme, I.N.R.A., 21034, Dijon Cédex, France

    Jean-Louis Sébédio

  3. Department of Food Science, Burnsides Research Laboratory, University of Illinois, 1208 W. Pennsylvania Ave., 61801, Urbana, IL

    Edward G. Perkins

Authors
  1. Courdjo Lamboni
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  2. Jean-Louis Sébédio
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  3. Edward G. Perkins
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Correspondence to Edward G. Perkins.

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Lamboni, C., Sébédio, JL. & Perkins, E.G. Cyclic fatty acid monomers from dietary heated fats affect rat liver enzyme activity. Lipids 33, 675–681 (1998). https://doi.org/10.1007/s11745-998-0256-0

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  • DOI: https://doi.org/10.1007/s11745-998-0256-0

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