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Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding

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Lipids

Abstract

We have developed cultures of smooth muscle cells (SMC) isolated from arterial hypercholesterolemic chicks (cholesterol-SMC). These cultures are suitable for the study at the molecular level of the changes in arterial SMC induced by a cholesterol diet. By using a strong dose of cholesterol (5%) for 10 d, we obtained very proliferative SMC which became foam cells after 30 d in culture. On the other hand, SMC cultures isolated from control-fed chicks has a lower growth rate than the SMC ones under the same culture conditions. DNA synthesis was fourfold greater in cholesterol-SMC than in control-SMC cultures. Intracellular cholesterol concentrations were the same in both cholesterol and control SMC during the first 14 d of culture but afterward increased in differing ways: after 20 d of culture the cholesterol-SMC increased their cholesterol content to double the control. We give here the results obtained from transmission electron microscopy, lipid analysis, proliferation studies, DNA, RNA and protein synthesis, and then discuss their implications.

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Abbreviations

DMEM:

Dulbecco's modification of Eagle's medium

FCS:

fetal calf serum

HDL:

high density lipoprotein

LDL:

low density lipoprotein

PBS:

phosphate-buffered saline

SMC:

smooth muscle cell

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Authors and Affiliations

  1. Department of Cell Biology, Faculty of Sciences, University of Granada, 18071, Granada, Spain

    Antonio Ríos

  2. Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Granada, Fuentenueva sn, 18071, Granada, Spain

    Angel Carazo, M. José Alejandre, Ramón Diaz, Mercedes Castillo & Ana Linares

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  1. Angel Carazo
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  2. M. José Alejandre
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  3. Ramón Diaz
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  4. Antonio Ríos
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  5. Mercedes Castillo
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  6. Ana Linares
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Correspondence to Ana Linares.

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Carazo, A., Alejandre, M.J., Diaz, R. et al. Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding. Lipids 33, 181–190 (1998). https://doi.org/10.1007/s11745-998-0194-x

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  • DOI: https://doi.org/10.1007/s11745-998-0194-x

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