The effects of conjugated linoleic acid (CLA) on body composition were investigated. ICR mice were fed a control diet containing 5.5% corn oil or a CLA-supplemented diet (5.0% corn oil plus 0.5% CLA). Mice fed CLA-supplemented diet exhibited 57% and 60% lower body fat and 5% and 14% increased lean body mass relative to controls (P<0.05). Total carnitine palmitoyltransferase activity was increased by dietary CLA supplementation in both fat pad and skeletal muscle; the differences were significant for fat pad of fed mice and skeletal muscle of fasted mice. In cultured 3T3-L1 adipocytes CLA treatment (1×10−4 M) significantly reduced heparin-releasable lipoprotein lipase activity (−66%) and the intracellular concentrations of triacylglyceride (−8%) and glycerol (−15%), but significantly increased free glycerol in the culture medium (+22%) compared to control (P<0.05). The effects of CLA on body composition appear to be due in part to reduced fat deposition and increased lipolysis in adipocytes, possibly coupled with enhanced fatty acid oxidation in both muscle cells and adipocytes.
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conjugated linoleic acid
Dulbecco’s modified Eagle’s medium
Chin, S.F., Liu, W., Storkson, J.M., Ha, Y.L., and Pariza, M.W. (1992) Dietary Sources of Conjugated Dienoic Isomers of Linoleic Acid, a Newly Recognized Class of Anticarcinogens, J. Food Comp. Anal. 5, 185–197.
Ha, Y.L., Grimm, N.K., and Pariza, M.W. (1987) Anticarcinogens from Fried Ground Beef: Heat-Altered Derivatives of Linoleic Acid, Carcinogenesis 8, 1881–1887.
Ha, Y.L., Grimm, N.K., and Pariza, M.W. (1989) Newly Recognized Anticarcinogenic Fatty Acids: Identification and Quantification in Natural and Processed Cheeses, J. Agric. Food Chem. 37, 75–81.
Ha, Y.L., Storkson, J., and Pariza, M.W. (1990) Inhibition of Benzo (α) pyrene-Induced Mouse Forestomach Neoplasia by Conjugated Dienoic Derivatives of Linoleic Acid, Cancer Res. 50, 1097–1101.
Ip, C., Chin, S.F., Scimeca, J.A., and Pariza, M.W. (1991) Mammary Cancer Prevention by Conjugated Dienoic Derivative of Linoleic Acid, Cancer Res. 51, 6118–6124.
Lee, K.N., Kritchevsky, D., and Pariza, M.W. (1994) Conjugated Linoleic Acid and Atherosclerosis in Rabbits, Atherosclerosis 108, 19–25.
Nicolosi, R.J., Courtemanche, K.V., Laitinen, L., Scimeca, J.A., and Huth, P.J. (1993) Effect of Feeding Diets Enriched in Conjugated Linoleic Acid on Lipoproteins and Aortic Atherogenesis in Hamsters, Circulation 88 (suppl.), 2458.
Cook, M.E., Miller, C.C., Park, Y., and Pariza, M.W. (1993) Immune Modulation by Altered Nutrient Metabolism: Nutritional Control of Immune-Induced Growth Depression, Poultry Sci. 72, 1301–1305.
Miller, C.C., Park, Y., Pariza, M.W., and Cook, M.E. (1994) Feeding Conjugated Linoleic Acid to Animals Partially Overcomes Catabolic Responses Due to Endotoxin Injection, Biochem. Biophys. Res. Commun. 198, 1107–1112.
Chin, S.F., Storkson, J.M., Albright, K.J., Cook, M.E., and Pariza, M.W. (1994) Conjugated Linoleic Acid Is a Growth Factor for Rats as Shown by Enhanced Weight Gain and Improved Feed Efficiency, J. Nutr. 124, 2344–2349.
Helrich, K. (1990) Official Methods of Analysis, 15th edn., pp. 935–937, Association of Official Analytical Chemists Inc., Arlington.
Frost, S.C., and Lane, M.D. (1985) Evidence for the Involvement of Vicinal Sulfhydryl Groups in Insulin-Activated Hexose Transport by 3T3-L1 Adipocytes, J. Biol. Chem. 260, 2646–2652.
Calder, P.C., Bond, J.A., Harvey, D.J., Gordon, S., and Newsholme, E.A. (1990) Uptake and Incorporation of Saturated and Unsaturated Fatty Acids into Macrophage Lipids and Their Effect upon Macrophage Adhesion and Phagocytosis, Biochem. J. 269, 807–814.
Martyn, P., and Hansen, I.A. (1981) Initiation of Lipogenic Enzyme Activities in Rat Mammary Glands, Biochem. J. 198, 187–192.
Lynen, F. (1962) Fatty Acid Synthesis from Malonyl CoA, Methods Enzymol. 1, 739–741.
Plumb, J.A., Milroy, R., and Kaye, S.B. (1989) Effects of the pH Dependence of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide-Formazan Absorption on Chemosensitivity Determined by a Novel Tetrazolium-based Assay, Cancer Res. 49, 4435–4440.
Nilsson-Ehle, P., and Schotz, M.C. (1976) A Stable, Radioactive Substrate Emulsion for Assay of Lipoprotein Lipase, J. Lipid Res. 17, 536–541.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem. 193, 265–275.
Bieber, L.L., Abraham, T., and Helmrath, T. (1972) A Rapid Spectrophotometric Assay for Carnitine Palmitoyltransferase, Anal. Chem. 50, 509–518.
Boissonneault, G.A., Elson, C.E., and Pariza, M.W. (1986) Net Energy Effects of Dietary Fat on Chemically Induced Mammary Carcinogenesis in F344 Rats, J. Natl. Cancer Inst. 76, 335–338.
Takada, R., Saitoh, M., and Mori, T. (1994) Dietary γ-Linolenic Acid-Enriched Oil Reduces Body Fat Content and Induces Liver Enzyme Activities Relating to Fatty Acid β-Oxidation in Rats, J. Nutr. 124, 469–474.
Pariza, M.W., Park, Y., Kim, S., Sugimoto, K., Albright, K., Liu, W., Storkson, J., and Cook, M. (1997) Mechanism of Body Fat Reduction by Conjugated Linoleic Acid, FASEB J. 11, A139.
Hotamisligil, G.S., Arner, P., Caro, J.F., Atkinson, R.L., and Spiegelman, B.M. (1995) Increased Adipose Tissue Expression of TNF-α in Human Obesity and Insulin Resistance, J. Clin. Invest. 95, 2409–2415.
Hotamisligil, G.S., Peraldi, P., Budavari, A., Ellis, R., White, M.F., and Spiegelman, B.M. (1996) IRS-1-Mediated Inhibition of Insulin Receptor Tyrosine Kinase Activity in TNF-α-and Obesity-Induced Insulin Resistance, Science 271, 665–668.
Hotamisligil, G.S., Shargill, N.S., and Spiegelman, B.M. (1993) Adipose Expression of Tumor Necrosis Factor-α: Direct Role in Obesity-Linked Insulin Resistance, Science 259, 87–91.
Bunout, D., Munoz, C., Lopez, M., de la Maza, M.P., Schlesinger, L., Hirsch, S., and Pettermann, M. (1996) Interleukin 1 and Tumor Necrosis Factor in Obese Alcoholics Compared with Normal-Weight Patients, Am. J. Clin. Nutr. 63, 373–376.
Parrish, C.C., Pathy, D.A., and Angel, A. (1990) Dietary Fish Oils Limit Adipose Tissue Hypertrophy in Rats, Metabolism 39, 217–219.
Awad, A.B., Bernardis, L.L., and Fink, C.S. (1990) Failure to Demonstrate an Effect of Dietary Fatty Acid Composition on Body Weight, Body Composition and Parameters of Lipid Metabolism in Mature Rats, J. Nutr. 120, 1277–1282.
Beck, S., Smith, K.L., and Tisdale, M.J. (1991) Anticachectic and Antitumor Effect of Eicosapentaenoic Acid and Its Effect on Protein Turnover, Cancer Res. 51, 6089–6093.
Hellerstein, M.K., Meydani, S.N., Meydani, M., Wu, K., and Dinarello, C.A. (1989) Interleukin-1-Induced Anorexia in the Rat, J. Clin. Invest. 84, 228–235.
Leslie, C.A., Gonnerman, W.A., Ullman, M.D., Hayes, K.C., Franzblau, C., and Cathcart, E.S. (1985) Dietary Fish Oil Modulates Macrophage Fatty Acids and Decreases Arthritis Susceptibility in Mice, J. Exp. Med. 162, 1336–1349.
Lokesh, B.R., Hsieh, H.L., and Kinsella, J.E. (1986) Peritoneal Macrophages from Mice Fed Dietary (n−3) Polyunsaturated Fatty Acids Secrete Low Levels of Prostaglandins, J. Nutr. 116, 2547–2552.
Endres, S., Ghorbani, R., Kelley, V.E., Georgilis, K., Lonnemann, G., van der Meer, J.W.M., Cannon, J.G., Rogers, T.S., Klempner, M.S., Weber, P.C., Schaefer, E.J., Wolff, S.M., and Dinarello, C.A. (1989) The Effect of Dietary Supplementation with n−3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononucler Cells, New Engl. J. Med. 320, 265–271.
Liew, C., Schut, H.A.J., Chin, S.F., Pariza, M.W., and Dashwood, R.H. (1995) Protection of Conjugated Linoleic Acids Against 2-Amino-3-methylimidazo [4.5-f]quinoline-Induced Colon Carcinogenesis in the F344 Rat: a Study of Inhibitory Mechanisms, Carcinogenesis 16, 3037–3043.
Belury, M.A. (1995) Conjugated Dienoic Linoleate: A Polyunsaturated Fatty Acid with Unique Chemoprotective Properties, Nutr. Rev. 53, 83–89.
Banni, S., Day, B.W., Evans, R.W., Corongin, F.P., and Lombardi, B. (1995) Detection of Conjugated Diene Isomers of Linoleic Acid in Liver Lipids of Rats Fed a Choline-Devoid Diet Indicates That the Diet Does Not Cause Lipid Peroxidation, Nutr. Biochem. 6, 281–289.
Cohen, L.A., Choi, K., and Wang, C.-X. (1988) Influence of Dietary Fat, Caloric Restriction, and Voluntary Exercise on N-Nitrosomethylurea-Induced Mammary Tumorigenesis in Rats, Cancer Res. 48, 4276–4283.
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Park, Y., Albright, K.J., Liu, W. et al. Effect of conjugated linoleic acid on body composition in mice. Lipids 32, 853–858 (1997). https://doi.org/10.1007/s11745-997-0109-x
- Linoleic Acid
- Body Composition
- Conjugate Linoleic Acid
- Feed Intake
- Free Glycerol