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Thermotropic phase behavior of in vivo extracted human stratum corneum lipids

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Lipids

Abstract

The thermotropic phase behavior of lipids extracted either in vivo from inner forearm (SCLE) or plantar callus (PC) was investigated by differential scanning calorimetry and small angle X-ray diffraction. PC composition was chromatographically modified (MPC) by eliminating the more polar lipids in order to evaluate their role. Analysis of composition confirms the potential use of PC as a source of stratum corneum lipids. MPC and SCLE exhibit similar differential scanning calorimetry (DSC) profiles with a main transition around 50°C attributed to the solid-to-liquid phase transition of the ceramides. The absence of a transition around 50°C for PC suggests the possible perturbation of ceramide packing by the significantly high proportion of phospholipids. X-ray data suggest a high miscibility of sebum components in stratum corneum lipids with possible modification of chain packing. The MPC patterns show a lipid phase separation which underscores the role of polar lipids in cholesterol/free fatty acids/sterol esters/ceramides structural cohesion.

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Abbreviations

DSC:

differential scanning calorimetry

FFA:

free fatty acids

HPTLC-AMD:

high-performance thin-layer chromatography-automated multiple development

MPC:

modified plantar callus extract

PC:

plantar callus

SC:

stratum corneum

SCLE:

stratum corneum lipid extract

TG:

triglycerides

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Author notes

  1. Alain Meybeck

    Present address: LVMH Recherche, 25L rue des Peupliers, 92752, Nanterre, France

Authors and Affiliations

  1. Parfums Christian Dior, 45804, St. Jean de Braye, France

    Frédéric Bonté, Patrick Pinguet, Alex Saunois & Alain Meybeck

  2. Equipe Physicochimie des Systèmes Polyphasés, CNRS URA 1218 Université Paris-Sud, 92296, Châtenay-Malabry, France

    Sophie Beugin, Michel Ollivon & Sylviane Lesieur

Authors
  1. Frédéric Bonté
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  2. Patrick Pinguet
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  4. Alain Meybeck
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  6. Michel Ollivon
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  7. Sylviane Lesieur
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Bonté, F., Pinguet, P., Saunois, A. et al. Thermotropic phase behavior of in vivo extracted human stratum corneum lipids. Lipids 32, 653–660 (1997). https://doi.org/10.1007/s11745-997-0083-3

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  • DOI: https://doi.org/10.1007/s11745-997-0083-3

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