, Volume 52, Issue 9, pp 781–787 | Cite as

Anti-Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF-κB and MAP-Kinase Pathways in Macrophages

  • Jeehye Sung
  • Heemang Jeon
  • In-Hwan Kim
  • Heon Sang Jeong
  • Junsoo LeeEmail author


Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.


Stearidonic acid Echium oil Anti-inflammatory NFκB (nuclear factor κB) MAPK (mitogen-activated protein kinases) pathway 



α-Linolenic acid


Bovine serum albumin


Docosahexaenoic acid


Dulbecco’s modified Eagle’s medium


Foetal bovine serum


Eicosapentaenoic acid


Extracellular signal regulated kinase


c-jun N terminal kinase


Inducible nitric oxide synthase


Inhibitor of κB


Lactate dehydrogenase




Mitogen-activated protein kinases


3-(4,5-Dimethyl thiazol 2-yl)-2,5-diphenyltetrazolium


Nitric oxide


Nuclear factor κB

n-3 PUFA

Omega-3 polyunsaturated fatty acids


Dulbecco’s phosphate-buffered saline


Stearidonic acid


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Copyright information

© AOCS 2017

Authors and Affiliations

  • Jeehye Sung
    • 1
  • Heemang Jeon
    • 2
  • In-Hwan Kim
    • 3
  • Heon Sang Jeong
    • 1
  • Junsoo Lee
    • 1
    Email author
  1. 1.Division of Food and Animal Sciences, College of Agriculture, Life, and Environmental SciencesChungbuk National UniversityCheongjuKorea
  2. 2.Research and Innovation CenterCosmax Bio Inc.SeongnamKorea
  3. 3.Department of Food and NutritionKorea UniversitySeoulKorea

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