, Volume 52, Issue 8, pp 665–673 | Cite as

Medium-Chain Enriched Diacylglycerol (MCE-DAG) Oil Decreases Body Fat Mass in Mice by Increasing Lipolysis and Thermogenesis in Adipose Tissue

  • Haeun Kim
  • Jee-Hwan Choe
  • Jong Hun Choi
  • Hun Jung Kim
  • Soo Hyun Park
  • Moon Won Lee
  • Wooki Kim
  • Gwang-woong Go
Original Article


Medium chain fatty acid (MCFA) escapes the formation of chylomicrons in the small intestine, resulting in energy expenditure through beta-oxidation. Diacylglycerol (DAG) is susceptible to oxidation rather than being stored in the adipose tissue. This study was conducted to verify the effect of MCE-DAG oil on body fat mass in vivo. Male C57BL/6 mice were randomly assigned to four groups (n = 12) as follows: (1) normal diet (18% kcal from fat), (2) canola oil as a control (40% kcal from canola oil), (3) MCE-DAG10 (10% kcal from MCE-DAG + 30% kcal from canola oil), and (4) MCE-DAG20 (20% kcal from MCE-DAG + 20% kcal from canola oil). The body weight and fat mass of MCE-DAG20 group mice were decreased relative to those of control mice (P < 0.05 and P < 0.001, respectively). Serum triacylglycerol (TAG) was decreased in both MCE-DAG10 and MCE-DAG20 groups (P < 0.01 and P < 0.05, respectively). Hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) were increased in the MCE-DAG20 group relative to the control in white adipose tissue (WAT) (P < 0.05). Uncoupling protein 1 (UCP1) was also increased in the MCE-DAG20 group relative to the control in brown adipose tissue (BAT) (P < 0.05). In summary, MCE-DAG reduced body fat mass likely by stimulating lipolysis in WAT and thermogenesis in BAT.


Medium-chain enriched diacylglycerol Body fat mass Lipolysis Thermogenesis 



Acetyl CoA carboxylase


Adipose triglyceride lipase


Brown adipose tissue




Dual-energy X-ray absorptiometry


Fatty acid synthase


Glyceraldehyde-3-phophate dehydrogenase


Hormone sensitive lipase


Long-chain triacylglycerol


Low-density lipoprotein cholesterol




Medium-chain enriched diacylglycerol


Medium-chain fatty acid


Medium-chain triacylglycerol


Medium- and long-chain triacylglycerol


Monounsaturated fatty acid


Polyunsaturated fatty acid


Saturated fatty acid


Sterol regulatory element binding protein 1




Total cholesterol


Uncoupling protein 1


White adipose tissue

Supplementary material

11745_2017_4277_MOESM1_ESM.docx (4.1 mb)
Supplementary material 1 (DOCX 4166 kb)


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Copyright information

© AOCS 2017

Authors and Affiliations

  • Haeun Kim
    • 1
  • Jee-Hwan Choe
    • 1
  • Jong Hun Choi
    • 2
  • Hun Jung Kim
    • 2
  • Soo Hyun Park
    • 2
  • Moon Won Lee
    • 3
  • Wooki Kim
    • 4
  • Gwang-woong Go
    • 1
  1. 1.Department of Foods and NutritionKookmin UniversitySeoulKorea
  2. 2.R&D Center, NongshimSeoulKorea
  3. 3.Department of Public Health ScienceKorea UniversitySeoulKorea
  4. 4.Department of Food Science and BiotechnologyKyung Hee UniversityYonginKorea

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