Lipids

, Volume 52, Issue 8, pp 703–709 | Cite as

Lipid transfers to HDL are diminished in long-term bedridden patients: association with low HDL-cholesterol and increased inflammatory markers

  • Wilson Pascoalino Camargo de Oliveira
  • Thauany Martins Tavoni
  • Fatima Rodrigues Freitas
  • Bruna Miranda Oliveira Silva
  • Raul Cavalcante Maranhão
Original Article
  • 201 Downloads

Abstract

Plasma lipids have been extensively studied in sedentary and in subjects practicing exercise training, but not in extreme inactivity as occurs in bedridden patients. This is important for the care of bedridden patients and understanding the overall plasma lipid regulation. Here, we investigated plasma lipids, lipid transfers to HDL and inflammatory markers in bedridden patients. Fasting blood samples were collected from 23 clinically stable bedridden patients under long-term care (>90 days) and 26 normolipidemic sedentary subjects, paired for age and gender. In vitro transfer of four lipids to HDL was performed by incubating plasma with donor nanoparticles containing radioactive lipids. Total (193 ± 36 vs 160 ± 43, p = 0.005), LDL (124 ± 3 vs 96 ± 33 p = 0.003) and HDL-cholesterol (45 ± 10 vs 36 ± 13, p = 0.008), apolipoprotein A-I (134 ± 20 vs 111 ± 24, p = 0.001) and oxidized LDL (53 ± 13 vs 43 ± 12, p = 0.011) were lower in bedridden patients, whereas triglycerides, apolipoprotein B, CETP and LCAT were equal in both groups. Transfers of all lipids, namely unesterified cholesterol, cholesterol esters, triglycerides and phospholipids, to HDL were lower in bedridden patients, probably due to their lower HDL-cholesterol levels. Concentrations of IL-1β, IL-6, IL-8, HGF and NGF were higher in bedridden patients compared to sedentary subjects. In conclusion, inactivity had great impact on HDL, by lowering HDL-cholesterol, apolipoprotein A-I and thereby cholesterol transfers to the lipoprotein, which suggests that inactivity may deteriorate HDL protection beyond the ordinary sedentary condition.

Keywords

High-density lipoproteins (HDL) Lipid metabolism Cholesterol ester transfer protein (CETP) Exercise training Nanoparticles 

Abbreviations

apo

Apolipoprotein

BMI

Body mass index

CETP

Cholesterol ester transfer protein

HDL

High density lipoprotein

HGF

Hepatocyte growth factor

IL

Interleukin

LDL

Low density lipoprotein

LCAT

Lecithin-cholesterol acyltransferase

MCP-1

Monocyte chemotactic protein

NGF

Nerve growth factor

RCT

Reverse cholesterol transport

TNF-α

Tumor necrosis factor-α

Notes

Acknowledgements

The authors are thankful to Mrs. Josefa M.H.S. Lima BSc, for her help with the experiments.

Authors contribution

WPCO: patient selection, data collection, analysis and interpretation of data, manuscript writing. TMT: performance of experiments, analysis of data, manuscript writing. FRF: analysis of data, interpretation of results, manuscript writing. BMOS: performance of experiments. RCM: design and conduction of the study, manuscript writing, intellectual content. All authors read and approved the final manuscript.

Compliance with ethical standards

Funding

This study was supported by the São Paulo State Research Support Foundation (FAPESP, São Paulo, Brazil; Grant 14/03742-0). Dr. Maranhão has a Research Carrier Award from the National Council for Scientific and Technological Development (CNPq, Brasilia, Brazil).

Conflict of interest

The authors declare have no conflicts of interest.

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Copyright information

© AOCS 2017

Authors and Affiliations

  • Wilson Pascoalino Camargo de Oliveira
    • 1
    • 2
    • 3
  • Thauany Martins Tavoni
    • 1
    • 2
  • Fatima Rodrigues Freitas
    • 1
  • Bruna Miranda Oliveira Silva
    • 1
  • Raul Cavalcante Maranhão
    • 1
    • 2
    • 4
  1. 1.Lipid Metabolism Laboratory, Heart Institute (InCor), Medical School HospitalUniversity of São PauloSão PauloBrazil
  2. 2.Faculty of Pharmaceutical SciencesUniversity of São PauloSão PauloBrazil
  3. 3.Suzano Auxiliary Hospital, Medical School Hospital, University of São PauloSuzanoBrazil
  4. 4.Instituto do Coração (InCor) do Hospital das Clínicas, FMUSPSão PauloBrazil

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