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High Content of Dicranin in Anisothecium spirale (Mitt.) Broth., a Moss from Eastern Himalayas and Its Chemotaxonomic Significance

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Lipids

Abstract

Chemical investigation of Anisothecium spirale (Mitt.) Broth. (Family Dicranaceae), an epiphytic moss from the Darjeeling district hill region of the Eastern Himalayas was performed for the first time. Analysis of neutral lipid and phospholipid classes as well as their respective fatty acids revealed the presence of a significantly high content of octadeca-6-yn-9,12,15-trienoic acid, an acetylenic fatty acid commonly known as dicranin. Dicranin and a less abundant acetylenic fatty acid were detected in neutral lipids. The unique nature of the fatty acid signature supports the view of considering dicranin as chemotaxonomic marker for Dicranaceae members.

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Fig. 1
Fig. 2

Abbreviations

AFA:

Acetylenic fatty acid(s)

FA:

Fatty acid(s)

FAME:

Fatty acid methyl ester(s)

FFA:

Free fatty acid(s)

GC–MS:

Gas chromatography mass spectrometry

HP-TLC:

High performance thin layer chromatography

MUFA:

Monounsaturated fatty acid(s)

NL:

Neutral lipid(s)

PL:

Phospholipid(s)

PUFA:

Polyunsaturated fatty acid(s)

SFA:

Saturated fatty acid(s)

TAG:

Triacylglycerol(s)

TLC:

Thin layer chromatography

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Acknowledgements

The author is grateful to Prof. Mousumi Poddar-Sarkar of the University of Calcutta for sharing her expertise on MS analysis and for her critical comments during preparation of the manuscript. Instrumental support from the University Grants Commission-Centre of Advanced Study (UGC-CAS) and the Department of Science and Technology Fund for Improvement of S & T Infrastructure in Higher Educational Institutions (DST- FIST), Government of India, are also acknowledged.

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  1. Department of Botany, Darjeeling Government College, Darjeeling, 734101, India

    Souvik Mitra

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  1. Souvik Mitra
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Correspondence to Souvik Mitra.

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Mitra, S. High Content of Dicranin in Anisothecium spirale (Mitt.) Broth., a Moss from Eastern Himalayas and Its Chemotaxonomic Significance. Lipids 52, 173–178 (2017). https://doi.org/10.1007/s11745-017-4231-8

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