Lipids

, Volume 51, Issue 6, pp 743–755 | Cite as

Dietary Lipid and Carbohydrate Interactions: Implications on Lipid and Glucose Absorption, Transport in Gilthead Sea Bream (Sparus aurata) Juveniles

  • Carolina Castro
  • Geneviève Corraze
  • Ana Basto
  • Laurence Larroquet
  • Stéphane Panserat
  • Aires Oliva-Teles
Original Article

Abstract

A digestibility trial was performed with gilthead sea bream juveniles (IBW = 72 g) fed four diets differing in lipid source (fish oil, FO; or a blend of vegetable oil, VO) and starch content (0 %, CH−; or 20 %, CH+) to evaluate the potential interactive effects between carbohydrates and VO on the processes involved in digestion, absorption and transport of lipids and glucose. In fish fed VO diets a decrease in lipid digestibility and in cholesterol (C), High Density Lipoprotein(HDL)-C and Low Density Lipoprotein (LDL)-C (only in CH+ group) were recorded. Contrarily, dietary starch induced postprandial hyperglycemia and time related alterations on serum triacylglycerol (TAG), phospholipid (PL) and C concentrations. Fish fed a CH+ diet presented lower serum TAG than CH− group at 6 h post-feeding, and the reverse was observed at 12 h post-feeding for TAG and PL. Lower serum C and PL at 6 h post-feeding were recorded only in VOCH+ group. No differences between groups were observed in hepatic and intestinal transcript levels of proteins involved in lipid transport and hydrolysis (FABP, DGAT, GPAT, MTP, LPL, LCAT). Lower transcript levels of proteins related to lipid transport (ApoB, ApoA1, FABP2) were observed in the intestine of fish fed the CH+ diet, but remained unchanged in the liver. Overall, transcriptional mechanisms involved in lipid transport and absorption were not linked to changes in lipid serum and digestibility. Dietary starch affected lipid absorption and transport, probably due to a delay in lipid absorption. This study suggests that a combination of dietary VO and starch may negatively affect cholesterol absorption and transport.

Keywords

Alternative ingredients Digestibility Gene expression Nutrient absorption and transport Serum metabolites 

Abbreviations

ADC

Apparent digestibility coefficients

Apo

Apolipoproteins

CH

Carbohydrates

C

Cholesterol

C18 PUFA

Polyunsaturated fatty acids with 18 carbons

DGAT

Diacylglycerol acyltransferase

DHA

Docosahexaenoic acid

EPA

Eicosapentaenoic acid

FA

Fatty acid

FABP

Fatty acid binding protein

FM

Fish meal

FO

Fish oil

GLU

Glucose

GPAT

Glycerol-3-phosphate acyltransferase

G3P

Glycerol-3-phosphate

HDL

High-density lipoproteins

LCAT

Lecithin-cholesterol acyltransferase

LDL

Low-density lipoproteins

LC-PUFA

Long-chain polyunsaturated fatty acids

LPL

Lipoprotein lipase

MTP

Microsomal triglyceride transfer protein

MUFA

Monounsaturated fatty acids

NEFA

Non-esterified fatty acids

PL

Phospholipid

SFA

Saturated fatty acids

TAG

Triacylglycerol

VLDL

Very low density lipoprotein

VO

Vegetable oil

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Copyright information

© AOCS 2016

Authors and Affiliations

  • Carolina Castro
    • 1
    • 2
  • Geneviève Corraze
    • 3
  • Ana Basto
    • 1
  • Laurence Larroquet
    • 3
  • Stéphane Panserat
    • 3
  • Aires Oliva-Teles
    • 1
    • 2
  1. 1.Departamento de Biologia, Faculdade de CiênciasUniversidade do PortoPortoPortugal
  2. 2.CIMAR/CIIMAR-Centro Interdisciplinar de Investigação Marinha e AmbientalUniversidade do PortoPortoPortugal
  3. 3.INRAUR1067 Nutrition Metabolism AquacultureSaint-Pée-Sur-NivelleFrance

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