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Erratum to: Baker’s Yeast Deficient in Storage Lipid Synthesis Uses cis‑Vaccenic Acid to Reduce Unsaturated Fatty Acid Toxicity

The Original Article was published on 24 April 2015

Erratum to: Lipids DOI 10.1007/s11745-015-4022-z

In the original publication of the article, the Figs. 3, 4, 5 and 6 were swapped and published incorrectly. It is now corrected. The correct orders of Figs. 3, 4, 5 and 6 are given below.

Fig. 3
figure 3

Ability to release FA prevents production of cis-vaccenic acid in QM cells. Cells were grown 48 h in YNB complete liquid media and FA content in cells and cultivation media was analyzed as described in “Materials and Methods”. a Amount of individual FA in cells. b Amount of individual FA released to the medium. BY4742, wild type; QM, quadruple mutant; QM faa1,4Δ, quadruple mutant with faa1Δ and faa4Δ; 16:0, palmitic acid; 16:1n-7, palmitoleic acid; 18:0, stearic acid; 18:1n-9, oleic acid; 18:1n-7, cis-vaccenic acid. Data represent mean ± SD from five independent experiments. Statistical analysis was performed as described in “Materials and Methods”. a VA levels in QM cells compared to BY4742 cells, P ≤ 0.0001; b VA levels in QM faa1,4Δ cells compared to QM cells, P ≤ 0.0001; c VA levels in QM faa1,4Δ cells compared to BY4742 cells, P ≤ 0.0001

Fig. 4
figure 4

Effect of exogenous cis-vaccenic acid on growth of QM cells. Sensitivity of the BY4742 strain and from it derived quadruple mutant (QM) strain to external FA was established by drop tests on plates in serial dilutions. 5-μL aliquots of diluted cell suspensions containing 104–103–102–101 cells were spotted on YEPD agar plates containing 0.005 % of external FA and incubated at 28 °C for 2 days. 16:0, palmitic acid; 16:1n-7, palmitoleic acid; 18:0, stearic acid; 18:1n-9, oleic acid; 18:1n-7, cis-vaccenic acid

Fig. 5
figure 5

Supplementation of cis-vaccenic acid partially restores the growth of QM cells on oleic acid-containing media. Sensitivity of the BY4742 strain and from it derived quadruple mutant (QM) to external FA was established by drop tests on plates in serial dilutions. 5-μL aliquots of diluted cell suspensions containing 104–103–102–101 cells were spotted on YEPD agar plates with indicated concentrations of external FA and incubated at 28 °C for 2 days. 16:0, palmitic acid; 16:1n-7, palmitoleic acid; 18:0, stearic acid; 18:1n-9, oleic acid; 18:1n-7, cis-vaccenic acid

Fig. 6
figure 6

Externally added cis-vaccenic acid is efficiently imported into the cells and does not compete with oleic acid uptake. Cells were grown 24 h in YNB complete liquid media and FA content in cells was analyzed as described in “Materials and Methods”. a Cell growth. b Cellular content of individual FA. QM, quadruple mutant; QM faa1,4Δ, quadruple mutant with faa1Δ and faa4Δ; 16:0, palmitic acid; 16:1n-7, palmitoleic acid; 18:0, stearic acid; 18:1n-9, oleic acid (OA); 18:1n-7, cis-vaccenic acid (VA). Data represent mean ± SD from three independent experiments

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Correspondence to Roman Holic.

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The online version of the original article can be found under doi:10.1007/s11745-015-4022-z.

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Sec, P., Garaiova, M., Gajdos, P. et al. Erratum to: Baker’s Yeast Deficient in Storage Lipid Synthesis Uses cis‑Vaccenic Acid to Reduce Unsaturated Fatty Acid Toxicity. Lipids 50, 631–632 (2015). https://doi.org/10.1007/s11745-015-4040-x

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