Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639
Article
CAS
PubMed
Google Scholar
Sirevåg R, Levine RP (1972) Fatty acid synthetase from Chlamydomonas reinhardi. J Biol Chem 247:2586–2591
PubMed
Google Scholar
Fan J, Andre C, Xu C (2011) A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii. FEBS Lett 585:1985–1991
Article
CAS
PubMed
Google Scholar
Fan J, Yan C, Andre C, Shanklin J, Schwender J, Xu C (2012) Oil accumulation is controlled by carbon precursor supply for fatty acid synthesis in Chlamydomonas reinhardtii. Plant Cell Physiol 53:1380–1390
Article
CAS
PubMed
Google Scholar
Knothe G (2009) Improving biodiesel fuel properties by modifying fatty ester composition. Energy Environ Sci 2:759–766
Article
CAS
Google Scholar
Stansell GR, Gray VM, Sym SD (2011) Microalgal fatty acid composition: implications for biodiesel quality. J Appl Phycol 24:791–801
Article
Google Scholar
Inui H, Miyatake K, Nakano Y, Kitaoka S (1982) Wax ester fermentation in Euglena gracilis. FEBS Lett 150:89–93
Article
CAS
Google Scholar
Inui H, Miyatake K, Nakano Y, Kitaoka S (1983) Production and composition of wax esters by fermentation of Euglena gracilis. Agric Biol Chem 47:2669–2671
Article
CAS
Google Scholar
Inui H, Miyatake K, Nakano Y, Kitaoka S (1984) Fatty acid synthesis in mitochondria of Euglena gracilis. Eur J Biochem 142:121–126
Article
CAS
PubMed
Google Scholar
Teerawanichpan P, Qiu X (2010) Fatty acyl-CoA reductase and wax synthase from Euglena gracilis in the biosynthesis of medium-chain wax esters. Lipids 45:263–273
Article
CAS
PubMed
Google Scholar
Fox AR, Soto G, Mozzicafreddo M, Garcia AN, Cuccioloni M, Angeletti M, Salerno JC, Ayub ND (2014) Understanding the function of bacterial and eukaryotic thiolases II by integrating evolutionary and functional approaches. Gene 533:5–10
Article
CAS
PubMed
Google Scholar
Oda Y, Nakano Y, Kitaoka S (1982) Utilization and toxicity of exogenous amino acids in Euglena gracilis. J Gen Microbiol 128:853–858
CAS
Google Scholar
Koren LE, Hutner SH (1967) High-yield media for photosynthesizing Euglena gracilis z. J Protozool 14(Supplement):17
Google Scholar
Sauch JF, Flanigan D, Galvin ML, Berman D, Jakubowski W (1991) Propidium iodide as an indicator of Giardia cyst viability. Appl Environ Microbiol 57:3243–3247
PubMed Central
CAS
PubMed
Google Scholar
O’Brien EA, Koski LB, Zhang Y, Yang L, Wang E, Gray MW, Burger G, Lang BF (2007) TBestDB: a taxonomically broad database of expressed sequence tags (ESTs). Nucleic Acids Res 35:D445–D451
Article
PubMed Central
PubMed
Google Scholar
Nakazawa M, Minami T, Teramura K, Kumamoto S, Hanato S, Takenaka S, Ueda M, Inui H, Nakano Y, Miyatake K (2005) Molecular characterization of a bifunctional glyoxylate cycle enzyme, malate synthase/isocitratelyase, in Euglena gracilis. Comp Biochem Physiol B Biochem Mol Biol 141:445–452
Article
PubMed
Google Scholar
Tessier L, Keller M, Chan RL, Fournier R, Weil JH, Imbault P (1991) RNAs to pre-mature mRNAs by trans-splicing in Euglena. EMBO J 10:2621–2625
PubMed Central
CAS
PubMed
Google Scholar
Iseki M, Matsunaga S, Murakami A, Ohno K, Shiga K, Yoshida K, Sugai M, Takahashi T, Hori T, Watanabe M (2002) A blue-light-activated adenylyl cyclase mediates photo avoidance in Euglena gracilis. Nature 415:1047–1051
Article
CAS
PubMed
Google Scholar
Ishikawa T, Tajima N, Nishikawa H, Gao Y, Rapolu M, Shibata H, Sawa Y, Shigeoka S (2010) Euglena gracilisas corbate peroxidase forms an intramolecular dimeric structure: its unique molecular characterization. Biochem J 426:125–134
Article
CAS
PubMed
Google Scholar
Takenaka S, Kondo T, Nazeri S, Tamura Y, Tokunaga M, Tsuyama S, Miyatake K, Nakano Y (1997) Accumulation of trehalose as a compatible solute under osmotic stress in Euglena gracilis Z. J Eukaryot Microbiol 44:609–613
Article
CAS
Google Scholar
Hodge JE, Hofreiter BT (1962) Determination of reducing sugars and carbohydrates. In: Whistler RL, Wolfrom ML (eds) Methods in carbohydrate chemistry, vol 1. Academic press, New York, pp 380–394
Google Scholar
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Article
CAS
PubMed
Google Scholar
Christie WW (1993) Preparation of ester derivatives of fattyacids for chromatographic analysis. In: Christie WW (ed) Advances in lipid methodology—two. The Oily Press, Dundee, UK, pp 69–111
Google Scholar
Dunn RO (1999) Thermal analysis of alternative diesel fuels from vegetable oils. J Am Oil Chem Soc 76:109–115
Article
CAS
Google Scholar
Meriläinen G, Poikela V, Kursula P, Wierenga RK (2009) The thiolase reaction mechanism: the importance of Asn316 and His348 for stabilizing the enolate intermediate of the Claisen condensation. Biochemistry 48:11011–11025
Article
PubMed
Google Scholar
Emanuelsson O, Brunak S, von Heijne G, Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc 2:953–971
Article
CAS
PubMed
Google Scholar
Nakai K, Horton P (1999) PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem Sci 24:34–36
Article
CAS
PubMed
Google Scholar
Inui H, Ochi H, Nogami K, Miyatake K, Nakano Y, Kitaoka S (1988) Effect of thiamin deficiency on wax ester fermentation in Euglena gracilis. Agric Biol Chem 52:49–54
Article
CAS
Google Scholar
Thelen JJ, Ohlrogge JB (2002) Metabolic engineering of fatty acid biosynthesis in plants. Metab Eng 4:12–21
Article
CAS
PubMed
Google Scholar
Eccleston V, Ohlrogge J (1998) Expression of lauroyl-acyl carrier protein thioesterase in Brassica napus seeds induces pathways for both fatty acid oxidation and biosynthesis and implies a set point for triacylglycerol accumulation. Plant Cell 10:613–622
PubMed Central
CAS
PubMed
Google Scholar
Radakovits R, Eduafo P, Posewitz MC (2010) Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum. Metab Eng 13:89–95
Article
PubMed
Google Scholar
Dellomonaco C, Clomburg JM, Miller EN, Gonzalez R (2011) Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals. Nature 476:355–359
Article
CAS
PubMed
Google Scholar