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Single Nucleotide Polymorphism Scanning and Expression of the Pig PPARGC1A Gene in Different Breeds

  • Original Article
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Lipids

Abstract

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a candidate gene for lean meat production because it plays a key role in lipid metabolism. In this study, SNPs within the porcine PPARGC1A gene were investigated using PCR-sequencing and PCR-RFLP. Quantitative real-time PCR and Western blot were then used to analyze mRNA and protein expression in longissimus dorsi muscle (LM), liver, and backfat tissues of Dianna small-ear pigs (DSP, n = 6), Tibetan pigs (TP, n = 6), and large white pigs (LW, n = 6). Five novel SNPs (g.−1269A>G in the 5′-upstream regulatory region; g.190C>T, g.218C>A and g.234C>A in exon 8; and g.20C>T in intron 10) and three previously identified SNPs (g.417A>T in exon 8; g.56C>A in exon 9; and g.34G>A in intron 9) were found. Of these, only two, g.−1269A>G and g.234C>A, had three different genotypes in the three breeds (DSP, n = 63; TP, n = 51; and LW, n = 52). Expression was highest in LM, modest in the liver, and minimal in backfat. In LM tissue, LW had higher mRNA and protein levels than DSP and TP (P < 0.05), and there was a negative correlation between gene expression and intramuscular fat (IMF) content. LW had numerically higher expression in liver and backfat tissues than DSP and TP, and the differences in protein levels were significant (P < 0.05 in liver, P < 0.01 in backfat). In conclusion, PPARGC1A may play a key role in down-regulating lipid deposition, and the SNPs with differential genotype distribution among the three pig breeds may be related to gene expression and fat deposition.

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Abbreviations

IMF:

Intramuscular fat

SNP:

Single nucleotide polymorphisms

PCR-RFLP:

PCR-restriction fragment length polymorphism

LM:

Longissimus dorsi muscle

DSP:

Dianna small-ear pig

LW:

Large white pig

TP:

Tibetan pigs

QTL:

Quantitative trait loci

eECL:

Enhanced electrochemiluminescence

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Acknowledgments

This work was supported by the National Major Special Project on New Varieties Cultivation for Transgenic Organisms (2013ZX08009-003-006), the National Natural Science Foundation of China (U1036604) and Chinese Universities Scientific Fund (2013QT008). We also thank the Yunnan Agricultural University and Tibet Agricultural and Animal Sciences College for help in collecting the samples.

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Author notes

  1. Hao Zhang

    Present address: National Engineering Laboratory For Animal Breeding, China Agricultural University, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China

Authors and Affiliations

  1. National Engineering Laboratory For Animal Breeding, China Agricultural University, No. 2 Yuanmingyuan West Rd., Beijing, 100193, China

    Qinggang Li, Zhixiu Wang, Bo Zhang, Yuzeng Yang, Dongmei Ban & Changxin Wu

  2. Institute of Animal Sciences and Veterinary Medicine, Anhui Academy of Agricultural Sciences, No. 40 Nongke South Rd., Hefei, 230031, Anhui Province, China

    Qinggang Li

  3. School of Life Science and Technology, Nanyang Normal University, Nanyang, 473061, Henan Province, China

    Yunfeng Lu

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  1. Qinggang Li
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Correspondence to Hao Zhang.

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Li, Q., Wang, Z., Zhang, B. et al. Single Nucleotide Polymorphism Scanning and Expression of the Pig PPARGC1A Gene in Different Breeds. Lipids 49, 1047–1055 (2014). https://doi.org/10.1007/s11745-014-3928-1

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