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Headspace Volatile Oxylipins of Eastern Himalayan Moss Cyathophorella adiantum Extracted by Sample Enrichment Probe

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Lipids

Abstract

Cyathophorella adiantum (Griff.) M. Fleisch. (Division-Bryophyta, Family-Daltoniaceae), an Eastern Himalayan moss was studied for the first time to identify the volatiles derived from cellular and membrane bound fatty acids. A high capacity sample enrichment probe (SEP) was used for extraction of headspace volatile (HSV) molecules followed by GC–MS analysis. Different short-chain oxylipins like alkenes, alkanes, saturated and unsaturated alcohols, saturated and unsaturated aldehydes, ketones were identified along with free and esterified fatty acids, cyclo compounds and some by-products of secondary metabolites. Fatty acid analysis of neutral lipids (NL) and phospholipids (PL) of this plant exhibits the predominance of C16 and C18 fatty acids. It also reveals some interesting information that might indicate the possible fatty acid precursors for volatile generation and their sources in this plant.

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Abbreviations

FA:

Fatty acid(s)

FAME:

Fatty acids methyl ester(s)

HSV:

Headspace volatile(s)

LOX:

Lipoxygenase

NL:

Neutral lipid(s)

PL:

Phospholipid(s)

PDMS:

Polydimethylsiloxane

PUFA:

Polyunsaturated fatty acid(s)

SEP:

Sample enrichment probe

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Acknowledgments

We heartily acknowledge the kind assistance from The Programme Co-ordinator, Centre of Advanced Study and the Head of the Department of Botany for giving us infrastructural support. We are thankful to the Department of Science and Technology, the Government of West Bengal for funding. We deeply acknowledge the UGS-CAS and DST-FIST programme, Government of India for providing us with instrument facilities. We are grateful to Dr. Lopamudra Saha, Assistant Professor of the University of Kualu-Zulu, South Africa and Dr. Eke Zsuzsanna, Kramarics Aron of the Department of Chemistry, Eotvos Lorand University, Hungary for assisting us in many ways. We are indebted to the scientists who have given us their valuable time for critically reviewing this article.

Conflict of interest

The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Botany (Centre of Advanced Study), University of Calcutta, 35-Ballygunge Circular Road, Kolkata, 700019, India

    Souvik Mitra & Mousumi Poddar-Sarkar

  2. Department of Chemistry and Polymer Science, Stellenbosch University, Stellenbosch, 7600, South Africa

    Ben V. Burger

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  1. Souvik Mitra
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  2. Ben V. Burger
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  3. Mousumi Poddar-Sarkar
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Correspondence to Mousumi Poddar-Sarkar.

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Mitra, S., Burger, B.V. & Poddar-Sarkar, M. Headspace Volatile Oxylipins of Eastern Himalayan Moss Cyathophorella adiantum Extracted by Sample Enrichment Probe. Lipids 48, 997–1004 (2013). https://doi.org/10.1007/s11745-013-3807-1

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