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Porcine G0/G1 Switch Gene 2 (G0S2) Expression is Regulated During Adipogenesis and Short-Term In-Vivo Nutritional Interventions

  • Original Article
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Lipids

Abstract

Adipose triglyceride lipase (ATGL), catalyzing the initial step of hydrolysis of triacylglycerol (TAG) in adipocytes, has been known to be inhibited by G0/G1 switch gene 2 (G0S2). In this study, we report the porcine G0S2 cDNA and amino acid sequences as well as the expression level of porcine G0S2. The porcine G0S2 mRNA was abundantly expressed in adipose tissue and liver among various tissues. In adipose tissue, porcine G0S2 expression was 16-fold higher in the fat cell fraction than the stromal vascular fraction. The G0S2 level increased significantly during adipogenesis in vitro and in vivo. These data indicate that G0S2 expression is closely associated with lipid accumulation and adipogenesis. Considering G0S2 as an inhibitor of cell proliferation, the relatively low levels of G0S2 in preadipocytes and adipose tissues of fetal and neonatal pigs compared to adipocytes and adipose tissues of adult pigs may allow the fast cell proliferation rates. Further studies showed that a short-term 24-h fast down-regulated G0S2 expression and increased ATGL expression in adipose tissue; however, a long-term calorie restriction for 8 days had no influence on the level of G0S2 but increased ATGL expression. Therefore, porcine G0S2, which is both a negative regulator of ATGL-mediated lipolysis and cell proliferation in adipose tissue, can be down-regulated in vivo by a short-term 24-h fast followed by increased ATGL-mediated lipolysis.

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Abbreviations

AA:

Amino acid

ATGL:

Adipose triglyceride lipase

DAG:

Diacylglycerol

DLK1:

Delta-like 1 homolog

FBS:

Fetal bovine serum

FFA:

Free fatty acids

G0S2:

G0/G1 switch gene 2

HSL:

Hormone sensitive lipase

LPL:

Lipoprotein lipase

MAG:

Monoacylglycerol

NEFA:

Nonesterified fatty acids

PPARγ:

Peroxisome proliferator-activated receptor gamma

RT-PCR:

Reverse transcription polymerase chain reaction

SV:

Stromal vascular

TAG:

Triacylglycerol

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Acknowledgments

This work was supported by the National Research Foundation of Korea Grant funded by the Korean government (KRF-2009-220-F00006) and the Ohio Agricultural Research and Development Center Director’s Associateship Program.

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Authors and Affiliations

  1. Department of Animal Sciences, The Ohio State University, Columbus, OH, 43210, USA

    Jinsoo Ahn, Shin-Ae Oh, Yeunsu Suh, Steven J. Moeller & Kichoon Lee

  2. The Ohio State University Interdisciplinary Ph.D. Program in Nutrition, The Ohio State University, Columbus, OH, 43210, USA

    Jinsoo Ahn & Kichoon Lee

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  1. Jinsoo Ahn
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  2. Shin-Ae Oh
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  3. Yeunsu Suh
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Corresponding author

Correspondence to Kichoon Lee.

Electronic Supplementary Material

Below is the link to the electronic supplementary material.

11745_2013_3756_MOESM1_ESM.pdf

Multiple alignments of complete coding sequences of G0S2 from Human (GenBank accession NM_015714), Mouse (GenBank accession NM_008059) Berkshire (GenBank accession JQ_013998), Landrace (GenBank accession JQ_013999), and Duroc (GenBank accession JQ_014000). Identities to the human G0S2 nucleotide sequence are denoted by a period, and nucleotide differences among porcine species were indicated by an open box (PDF 83 kb)

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Ahn, J., Oh, SA., Suh, Y. et al. Porcine G0/G1 Switch Gene 2 (G0S2) Expression is Regulated During Adipogenesis and Short-Term In-Vivo Nutritional Interventions. Lipids 48, 209–218 (2013). https://doi.org/10.1007/s11745-013-3756-8

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  • DOI: https://doi.org/10.1007/s11745-013-3756-8

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