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Linolelaidic Acid Induces a Stronger Proliferative Effect on Human Umbilical Vein Smooth Muscle Cells Compared to Elaidic Acid

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Lipids

Abstract

Trans fatty acids (TFA) have been considered as an independent risk factor of coronary heart disease, sudden death and insulin-resistance, and different TFA isomers may have different effects on the progression of cardiovascular diseases such as atherosclerosis. The aim of the study was to investigate the effects of two major TFA, elaidic acid and linolelaidic acid which have the same number of carbons but a different number and configuration of trans bonds, on the proliferation of human umbilical vein smooth muscle cells (HUVSMC). Methyl thiazolyl tetrazolium and flow cytometry assays showed that the cell proliferation rose to 115.37 ± 0.39 and 117.5 ± 0.57 % and the cell number in the S phase of the cell cycle reached 27.7 ± 0.7 and 25.8 ± 2.8 % when treated with 50 μM elaidic acid and 20 μM linolelaidic acid, respectively. Quantitative real-time reverse transcriptase-polymerase chain reaction and Western blotting analyses showed that the two TFA increased the mRNA and protein expression levels of PCNA, CDK2 and Cyclin E in HUVSMC. Moreover, gas chromatography analysis showed that the total PUFA level of HUVSMC was lower after treatment with the two TFA, especially n-3 PUFA. These results suggested that linolelaidic acid exhibited a stronger proliferative effect on HUVSMC than elaidic acid, and regulation of CDK2 and Cyclin E may be important for the effect of the TFA on atherosclerosis.

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Abbreviations

CHD:

Coronary heart disease

DMEM:

Dulbecco’s modified eagle medium

FBS:

Fetal bovine serum

GC:

Gas chromatography

HUVSMC:

Human umbilical vein smooth muscle cells

LA:

Linoleic acid

OA:

Oleic acid

PUFA:

Polyunsaturated fatty acid(s)

SCD:

Sudden cardiac death

TFA:

Trans fatty acids

VSMC:

Vascular smooth muscle cells

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (30972482, 31060214), the Academic Leader Program of Jiangxi Province (2008DD00900), the Doctoral Fund of Ministry of Education of China (No. 20070403002), the Natural Science Foundation of Jiangxi Province (2008GQY0023), A Ph.D. subject fund from Education Department (20113601120004), the Natural Science Fund from Science and Technology Department of Jiangxi Province (20114BAB214016).

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  The authors have declared no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Institute for Advanced Study, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, Jiangxi, China

    Xiao-Ping Li, Ting Luo, Jing Li, Ya-Wei Fan, Rong Liu, Jiang-Ning Hu, Xiao-Ru Liu & Ze-Yuan Deng

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  1. Xiao-Ping Li
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  2. Ting Luo
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  3. Jing Li
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Correspondence to Jing Li or Ze-Yuan Deng.

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Li, XP., Luo, T., Li, J. et al. Linolelaidic Acid Induces a Stronger Proliferative Effect on Human Umbilical Vein Smooth Muscle Cells Compared to Elaidic Acid. Lipids 48, 395–403 (2013). https://doi.org/10.1007/s11745-012-3754-2

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  • DOI: https://doi.org/10.1007/s11745-012-3754-2

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