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trans-10,cis-12 Conjugated Linoleic Acid Enhances Endurance Capacity by Increasing Fatty Acid Oxidation and Reducing Glycogen Utilization in Mice

Lipids volume 47, pages 855–863 (2012)Cite this article

Abstract

The supplementation of conjugated linoleic acid (CLA) has been shown to improve endurance by enhancing fat oxidation during exercise in rodents and humans. This study was designed to investigate the isomer-specific effects of CLA on endurance capacity and energy metabolism in mice during exercise. Male 129Sv/J mice were divided into three dietary groups and fed treatment diet for 6 weeks; control, 0.5 % cis-9,trans-11 (c9,t11) CLA, or 0.5 % trans-10,cis-12 (t10,c12) CLA. Dietary t10,c12 CLA induced a significant increase in maximum running time and distance until exhaustion with a dramatic reduction of total adipose depots compared to a control group, but there were no significant changes in endurance with the c9,t11 CLA treatment. Serum triacylglycerol and non-esterified fatty acid concentrations were significantly lower in the t10,c12 fed mice after exercise compared to control and the c9,t11 CLA fed-animals. Glycogen contents in livers of the t10,c12 fed-mice were higher than those in control mice, concomitant with reduction of serum l-lactate level. There were no differences in non-exercise physical activity among all treatment groups. In addition, the mRNA expression levels of carnitine palmitoyl transferase 1β, uncoupling protein 2 and peroxisome proliferator-activated receptor δ (PPARδ) in skeletal muscle during exercise were significantly up-regulated by the t10,c12 CLA but not the c9,t11 CLA. These results suggest that the t10,c12 CLA is responsible for improving endurance exercise capacity by promoting fat oxidation with a reduction of the consumption of stored liver glycogen, potentially mediated via PPARδ dependent mechanisms.

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Abbreviations

AMPK:

AMP-activated protein kinase

BUN:

Blood urea nitrogen

CLA:

Conjugated linoleic acid

CPT1β:

Carnitine palmitoyl transferase 1β

IL-6:

Interleukin 6

LPL:

Lipoprotein lipase

NEFA:

Non-esterified fatty acid

NEPA:

Non-exercise physical activity

PPARδ:

Peroxisome proliferator-activated receptor δ

TAG:

Triacylglycerol

UCP2:

Uncoupling protein 2

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Acknowledgments

The authors thank Prof. Daeyoung Kim at the Department of Mathematics and Statistics, University of Massachusetts, Amherst for help with statistical assistance. We also thank Ms. Jayne M. Storkson for help preparing this manuscript and Ms. Dayeh Lee and Eunji Choi for assistance with the experiments. This material is based up on work supported by the National Institute of Food and Agriculture, US Department of Agriculture, the Massachusetts Agricultural Experimental Station and the Department of Food Science under Project No. MAS0201001529. Dr. Yeonhwa Park is one of the inventors of CLA use patents that are assigned to the Wisconsin Alumni Research Foundation.

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Authors and Affiliations

  1. Department of Food Science, University of Massachusetts, 102 Holdsworth Way, Amherst, MA, 01003, USA

    Jun Ho Kim, Jonggun Kim & Yeonhwa Park

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  1. Jun Ho Kim
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  2. Jonggun Kim
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  3. Yeonhwa Park
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Correspondence to Yeonhwa Park.

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Kim, J.H., Kim, J. & Park, Y. trans-10,cis-12 Conjugated Linoleic Acid Enhances Endurance Capacity by Increasing Fatty Acid Oxidation and Reducing Glycogen Utilization in Mice. Lipids 47, 855–863 (2012). https://doi.org/10.1007/s11745-012-3698-6

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  • DOI: https://doi.org/10.1007/s11745-012-3698-6

Keywords

  • Conjugated linoleic acid
  • Exercise
  • Fat oxidation
  • Endurance
  • Mice