Abstract
It is known that the ratio, the level of sphingosine-1-phosphate (S1P)/the level of ceramide (CER) determines survival of the cells. The aim of the present study was to examine the effect of myocardial infarction on the level of different sphingolipids in the uninfarcted area. The experiments were carried out on male Wistar rats: 1, control; 2, after ligation of the left coronary artery (infarct) and 3, sham operated. Samples of the uninfarcted area of the left ventricle were taken in 1, 6 and 24 h after the surgery. The level of sphingolipids, S1P, CER, sphingosine (SPH), sphinganine-1-phosphate (SPA1P) and sphinganine (SPA) was determined. The control values were (ng/mg), S1P-0.33 ± 0.03, SPH-1.02 ± 0.13, SPA1P-0.11 ± 0.01, SPA-0.28 ± 0.04, total CER-20.3 ± 1.8. In infarct, the level of S1P in the uninfarcted area was reduced by ~3 times in 1 and 6 h and decreased further in 24 h. The level of SPH decreased in 1 h and returned to the control thereafter. The total level of CER decreased in 6 h after the infarction. Sham surgery also produced changes in the level of certain sphingolipids. The ratio, the level of S1P/the level of CER was markedly reduced at each time point after the infarction. It is concluded that the reduction in the S1P/CER ratio could be responsible for increased apoptosis in the uninfarcted area after the myocardial infarction in the rat.
Similar content being viewed by others
Abbreviations
- S1P:
-
Sphingosine-1-phosphate
- CER:
-
Ceramide
- SPH:
-
Sphingosine
- SPA1P:
-
Sphinganine-1-phosphate
- SPA:
-
Sphinganine
References
Karliner JS (2009) Sphingosine kinase and sphingosine-1-phosphate in cardioprotection. J Cardiovasc Pharmacol 53:189–197. doi:10.1097/FJC.0b013e3181926706
Kennedy S, Kane KA, Pyne NJ, Pyne S (2009) Targeting sphingosine-1-phosphate signalling for cardioprotection. Curr Opinion Pharmacol 9:194–201. doi:10.1016/j.coph.2008.11.002
Knapp M (2011) Cardioprotective role of sphingosine-1-phosphate. J Physiol Pharmacol 62:601–607
Gundewar S, Lefer DJ (2008) Sphingolipid therapy in myocardial ischemia-reperfusion injury. Biochim Biophys Acta 1780:571–576. doi:10.1016/j.bbagen.2007.08.014
Vessey DA, Kelley M, Li L, Huang Y, Zhou HZ, Zhu BQ, Karliner JS (2006) Role of sphingosine kinase activity in protection of heart against ischemia reperfusion injury. Med Sci Monit 12:BR318–BR324
Cui J, Engelman RM, Maulik N, Das DK (2004) Role of ceramide in ischemic preconditioning. J Am Coll Surg 198:770–777. doi:10.1016/j.jamcollsurg.2003.12.016
Cordis GA, Yoshida T, Das DK (1998) HPTLC analysis of sphingomyelin, ceramide and sphingosine in ischemic/reperfused rat heart. J Pharm Biomed Anal 16:1189–1193. doi:10.1016/S0731-7085(97)00260-4
Argaud L, Prigent A-F, Chalabreysse L, Loufouat J, Lagarde M, Ovize M (2004) Ceramide in the antiapoptotic effect of ischemic preconditioning. Am J Physiol Heart Circ Physiol 286:H246–H251. doi:10.1152/ajpheart.00638.2003
Zhang DX, Fryer RM, Hsu AK, Gross GJ, Campbell WB, Li P-L (2001) Production and metabolism of ceramide in normal and ischemic-reperfused myocardium of rats. Basic Res Cardiol 96:267–274. doi:10.1007/s00395-012-0266-4
Bielawska AE, Shapiro JP, Jiang L, Melkonyan HS, Piot C, Wolfe CL, Tomei D, Hannun YA, Umansky SR (1997) Ceramide is involved in triggering of cardiomyocyte apoptosis induced by ischemia and reperfusion. Am J Pathol 151:1257–1263
Beręsewicz A, Dobrzyń A, Górski J (2002) Accumulation of specific ceramides in ischemic/reperfused rat heart: effect of ischemic preconditioning. J Physiol Pharmacol 53:371–382
Lupiński SL, Schlicker E, Pędzińska-Betiuk A, Malinowska B (2011) Acute myocardial ischemia enhances the vanilloid TRPV1 and serotonin 5-HT(3) receptor-mediated Bezold-Jarisch reflex in rats. Pharmacol Rep 63:1450–1459
Błachnio-Zabielska AU, Persson XM, Koutsari C, Zabielski P, Jensen MD (2012) An LC/MS/MS method for measuring the in vivo incorporation of plasma free fatty acids into intramyocellular ceramides in humans. Rapid Commun Mass Spectrom 26:1134–1140. doi:10.1002/rcm.6216
Gault CR, Obeid LM, Hannun YA (2010) An overview of sphingolipid metabolism: from synthesis to breakdown. Adv Exp Med Biol 688:1–23
Riboni L, Viani P, Bassi R, Prinetti A, Tettamanti G (1997) The role of sphingolipids in the process of signal transduction. Prog Lipid Res 36:153–195. doi:10.1016/S0163-7827(97)00008-8
Ito K, Anada Y, Tani M, Ikeda M, Sano T, Kihara A, Igarashi Y (2007) Lack of sphingosine 1-phosphate degrading enzymes in erythrocytes. Biochem Biophys Res Commun 357:212–217. doi:10.1016/j.bbrc.2007.03.123
Hänel P, Andréani P, Gräler MH (2007) Erythrocytes store and release sphingosine 1-phosphate in blood. FASEB J 21:1202–1209. doi:10.1096/fj.06-7433com
Kim RH, Takabe K, Milstien S, Spiegel S (2009) Export and functions of sphingosine-1-phosphate. Biochim Biophys Acta 179:692–696. doi:10.1016/j.bbalip.200902011
Yatomi Y, Ruan F, Hakomori S, Igarashi Y (1995) Sphingosine-1-phosphate: a platelet-activating sphingolipid released from agonist-stimulated human platelets. Blood 86:193–202
Andréani P, Gräler MH (2006) Comparative quantification of sphingolipids and analogs in biological samples by high-performance liquid chromatography after chloroform extraction. Anal Biochem 358:239–246. doi:10.1016/j.ab.2006.08.027
Spiegel S, Milstein S (2002) Sphingosine-1-phosphate, a key cell signaling molecule. J Biol Chem 277:25851–25854. doi:10.1074/jbc.R200007200
Baranowski M, Zabielski P, Błachnio A, Górski J (2008) Effect of exercise duration on ceramide metabolism in the rat heart. Acta Physiol 192:519–529. doi:10.1111/j.1748-1716.2007.01755.x
Dobrzyń A, Górski J (2002) Effect of acute exercise on the content of free sphinganine and sphingosine in different skeletal muscle types of the rat. Horm Metab Res 34:523–529. doi:10.1055/s-2002-34793
Cheng W, Kajstura J, Nitahara JA, Li B, Teiss K, Liu Y, Clark WA, Krajewski S, Reed JC, Olivetti G, Anversa P (1996) Programmed myocyte cell death affects the viable myocardium after infarction in rats. Exp Cell Res 226:316–327. doi:10.1006/excr.1996.0232
Simonis G, Wiedemann S, Schwarz K, Christ T, Sedding DG, Yu X, Marquetant R, Braun-Dullaeus RC, Ravens U, Strasser RH (2008) Chelerythine treatment influences the balance of pro and anti-apoptotic signaling pathways in the remote myocardium after infarction. Mol Cell Biochem 310:119–128. doi:10.1007/s11010-007-9672-6
Palojoki E, Saraste A, Ericsson A, Pulkki K, Kallajoki M, Voipio-Pulkki LM, Tikkanen I (2001) Cardiomyocyte apoptosis and ventricular remodeling after myocardial infarction in rats. Am J Physiol Heart Circ Physiol 280:H2726–H2731
Gangoiti P, Camacho L, Arana L, Ouro A, Granado MH, Brizuela L, Casas J, Fabriás G, Abad JL, Delgado A, Gómez-Muñoz A (2010) Control of metabolism and signaling of simple bioactive sphingolipids: implications in disease. Prog Lipid Res 49:316–334. doi:10.1016/j.plipres.2010.02.004
Huwiler A, Kotler T, Pfeilschifter J, Sandhoff K (2000) Physiology and pathophysiology of sphingolipid metabolism and signaling. Biochem Biophys Acta 1485:63–69. doi:10.1016/S1388-1981(00)00042-1
Bartke N, Hannun YA (2009) Bioactive sphingolipids: metabolism and function. J Lipid Res 50:S91–S96. doi:10.1194/jlr.R800080-JLR200
Acknowledgments
This work was supported by UMB grants 113-18664L, 114-18874L. We greatly appreciate Prof. Irena Kasacka for microscopic examination of the myocardium and Dr. Justyna Marciniak for determination of the ischemic area.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Knapp, M., Żendzian-Piotrowska, M., Kurek, K. et al. Myocardial Infarction Changes Sphingolipid Metabolism in the Uninfarcted Ventricular Wall of the Rat. Lipids 47, 847–853 (2012). https://doi.org/10.1007/s11745-012-3694-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11745-012-3694-x