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Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

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Lipids

Abstract

Liquid chromatography–electrospray tandem mass spectrometry (LC/ESI–MS/MS) was used to analyze phospholipids from three species of the anaerobic beer-spoilage bacterial genus Pectinatus. Analysis of total lipids by HILIC (Hydrophilic Interaction Liquid Chromatography) column succeeded in separating diacyl- and plasmalogen phospholipids. Plasmalogens were then analyzed by means of the ESI–MS/MS and more than 220 molecular species of four classes of plasmalogens (PlsCho (choline plasmalogen), PlsEtn (ethanolamine plasmalogen), PlsGro (glycerol plasmalogen), and PlsSer (serine plasmalogen)) were identified. Major molecular species were c-p19:0/15:0 PlsEtn and PlsSer, which accounted for more than 4% of the total lipids.

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Abbreviations

CID:

Collision-induced dissociation

DMSZ:

Deutsche Sammlung von Mikroorganismen und Zellkulturen (German Collection of Microorganisms and Cell Cultures)

ECL:

Equivalent chain length

FA:

Fatty acid(s)

FAME:

Fatty acid methyl ester(s)

GC-MS:

Gas chromatography–mass spectrometry

HILIC:

Hydrophilic Interaction Liquid Chromatography

LC/ESI–MS/MS:

Liquid chromatography–electrospray ionization tandem mass spectrometry

PtdOH:

Phosphatidic acid

PtdIns:

Phosphatidylinositol

PlsCho:

Choline plasmalogen

PlsEtn:

Ethanolamine plasmalogen

PlsGro:

Glycerol plasmalogen

PlsSer:

Serine plasmalogen

RIBM:

Research Institute of Brewing and Malting

RSD:

Relative standard deviation

TLC:

Thin layer chromatography

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Acknowledgments

This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (Project MSM2B08022 and MSM6046137305), Institutional Research Concept AV0Z50200510 and Research Centre 1M0570).

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague, Czech Republic

    Tomáš Řezanka & Karel Sigler

  2. Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, Technická 5, 166 28, Prague, Czech Republic

    Lucie Siristova

  3. Research Institute of Brewing and Malting, Lípová 511/15, 120 44, Prague, Czech Republic

    Dagmar Matoulková

Authors
  1. Tomáš Řezanka
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  2. Lucie Siristova
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  3. Dagmar Matoulková
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  4. Karel Sigler
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Correspondence to Tomáš Řezanka.

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Řezanka, T., Siristova, L., Matoulková, D. et al. Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium. Lipids 46, 765–780 (2011). https://doi.org/10.1007/s11745-011-3556-y

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