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Methods of Emulsifying Linoleic Acid in Biohydrogenation Studies In Vitro May Bias the Resulting Fatty Acid Profiles

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Lipids

Abstract

The effects of three emulsifying methods on ruminal fatty acid biohydrogenation (BH) in vitro were compared. Using a static in-vitro gas test system, four replicates of each treatment were incubated in buffered ruminal fluid. Hemicellulose (300 mg dry matter) was supplemented either with or without linoleic acid (9c12c-18:2, 5% in diet dry matter) and incubated for 4 and 24 h. Three methods of emulsifying 9c12c-18:2 were tested: (1) ethanol, (2) Tween® 80, and (3) sonication. The products were then compared to non-emulsified 9c12c-18:2. Out of the three emulsifying methods tested, ethanol and sonication resulted in stable 9c12c-18:2 emulsions, indicating good 9c12c-18:2 distribution, while the Tween® 80 emulsion was less stable. BH was strongly inhibited by treating 9c12c-18:2 with ethanol and sonication at different steps of the BH-pathway, resulting in changed concentrations of certain BH intermediates. The fatty acid profile generated from the major BH-pathways of 9c12c-18:2 with Tween® 80 was comparable to that without emulsification after 24 h of incubation. We conclude that it is not recommended to emulsify lipids before incubating them in vitro when investigating fatty acid BH. If emulsification of 9c12c-18:2 is necessary, Tween® 80 seems to be the method that interferes least with BH.

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Abbreviations

BH:

Biohydrogenation

FAME:

Fatty acid methyl esters

LNA:

Linoleic acid

PUFA:

Polyunsaturated fatty acids

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Acknowledgments

We owe our thanks to Prof. M. Kreuzer for his support in the planning of this experiment.

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Authors and Affiliations

  1. Institute of Plant, Animal and Agroecosystem Sciences, ETH Zurich, Universitätstrasse 2, 8092, Zurich, Switzerland

    Ratchaneewan Khiaosa-ard, Florian Leiber & Carla R. Soliva

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  1. Ratchaneewan Khiaosa-ard
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  2. Florian Leiber
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  3. Carla R. Soliva
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Correspondence to Carla R. Soliva.

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Khiaosa-ard, R., Leiber, F. & Soliva, C.R. Methods of Emulsifying Linoleic Acid in Biohydrogenation Studies In Vitro May Bias the Resulting Fatty Acid Profiles. Lipids 45, 651–657 (2010). https://doi.org/10.1007/s11745-010-3440-1

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  • DOI: https://doi.org/10.1007/s11745-010-3440-1

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