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Trans Fatty Acid-Induced NF-κB Activation Does Not Induce Insulin Resistance in Cultured Murine Skeletal Muscle Cells

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Lipids

An Erratum to this article was published on 30 April 2010

Abstract

Long-chain saturated fatty acids such as palmitic acid induce insulin resistance and NF-κB activation in skeletal muscle cells. Here we investigated the effects of long-chain fatty acid (FA) saturation and configuration on NF-κB activity and insulin sensitivity in cultured skeletal muscle cells. Of all tested unsaturated FAs, only elaidic acid (3-fold), cis9,trans11-CLA (3-fold) and trans10,cis12-CLA (13-fold) increased NF-κB transactivation in myotubes. This was not accompanied by decreased insulin sensitivity (measured as insulin-induced glucose uptake and GLUT4 translocation). We therefore conclude that FA-induced NF-κB activation is not sufficient for the induction of insulin resistance in skeletal muscle cells.

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Fig. 1
Fig. 2

Abbreviations

AA:

Arachidonic acid

BSA:

Bovine serum albumin

CLA:

Conjugated linoleic acid

DAG:

Diacylglycerol

DHA:

Docosahexaenoic acid

EPA:

Eicosapentaenoic acid

IL-6:

Interleukin-6

MUFA:

Monounsaturated fatty acids

NF-κB:

Nuclear factor kappa B

PUFA:

Polyunsaturated fatty acids

SFA:

Saturated fatty acids

TNF-α:

Tumor necrosis factor-alpha

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Author information

Authors and Affiliations

  1. Department of Human Biology, Nutrim School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, P.O. Box 616, 6200 MD, Maastricht, The Netherlands

    Pascal P. H. Hommelberg, Ronald P. Mensink & Jogchum Plat

  2. Department of Respiratory Medicine, Nutrim School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands

    Ramon C. J. Langen, Annemie M. W. J. Schols, Anon L. M. van Essen & Frank J. M. Snepvangers

  3. Top Institute Food and Nutrition, Wageningen, The Netherlands

    Pascal P. H. Hommelberg & Ronald P. Mensink

Authors
  1. Pascal P. H. Hommelberg
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  2. Ramon C. J. Langen
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Correspondence to Ramon C. J. Langen.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s11745-010-3419-y

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Hommelberg, P.P.H., Langen, R.C.J., Schols, A.M.W.J. et al. Trans Fatty Acid-Induced NF-κB Activation Does Not Induce Insulin Resistance in Cultured Murine Skeletal Muscle Cells. Lipids 45, 285–290 (2010). https://doi.org/10.1007/s11745-010-3388-1

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