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Dietary Source of Stearidonic Acid Promotes Higher Muscle DHA Concentrations than Linolenic Acid in Hybrid Striped Bass

  • Original Article
  • Published:
Lipids

Abstract

Rapid expansion of aquacultural production is placing increasing demand on fish oil supplies and intensified the search for alternative lipid sources. Many of the potential alternative sources contain low concentrations of long chain n-3 fatty acids and the conversion of dietary linolenic acid to longer chain highly unsaturated fatty acids is a relatively inefficient process in some species. A 6-week study was conducted to compare tissue fatty acid (FA) concentrations in hybrid striped bass fed either 18:3n-3 (α-linolenic acid; ALA) or 18:4n-3 (stearidonic acid; SDA). Hybrid striped bass were fed either a control diet containing fish oil, or diets containing ALA or SDA at three different levels (0.5, 1 and 2% of the diet). There were no significant differences in whole animal responses between fish fed ALA or SDA. Liver and muscle concentrations of ALA and SDA were responsive to dosages fed. However, only 22:6n-3 concentrations in muscle were significantly affected by dietary source of 18 carbon precursors. Muscle 22:6n-3 concentrations were significantly higher in fish fed SDA compared to fish fed ALA. Based on these data, it appears that feeding SDA can increase long chain n-3 fatty acid concentrations in fish muscle.

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Abbreviations

ALA:

Linolenic acid

ANOVA:

Analysis of variance

AA:

Arachidonic acid

DHA:

Docosahexaenoic acid

DPA:

Docosapentaenoic acid

EPA:

Eicosapentaenoic acid

EFA:

Essential fatty acid

FAME:

Fatty acid methyl esters

FA:

Fatty acid

FID:

Flame ionization detector

GC:

Gas chromatograph

HUFA:

Highly unsaturated fatty acid

IPF:

Intraperitoneal fat

LC-n-3 HUFA:

Long chain n-3 highly unsaturated fatty acids

MONO:

Total monounsaturated fatty acids

PSEM:

Pooled standard error of the mean

RLW:

Relative liver weight

SAT:

Total saturated fatty acid

SDA:

Stearidonic acid

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Acknowledgments

This study was supported by Purdue Agriculture Research Programs.

Author information

Author notes

  1. Anant S. Bharadwaj

    Present address: Novus International, 20 Research Park Drive, St. Charles, MO, 63304, USA

  2. Steven D. Hart

    Present address: Indiana Soybean Alliance, 5730 West 74th Street, Indianapolis, IN, 46278, USA

Authors and Affiliations

  1. Department of Forestry and Natural Resources, Purdue University, 715 West State Street, West Lafayette, IN, 47907-2061, USA

    Anant S. Bharadwaj, Steven D. Hart, Billie J. Brown & Paul B. Brown

  2. Lipid Chemistry and Molecular Biology Laboratory, Department of Basic Medical Science, Purdue University, West Lafayette, IN, 47907, USA

    Yong Li & Bruce A. Watkins

Authors
  1. Anant S. Bharadwaj
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  2. Steven D. Hart
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  4. Yong Li
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Correspondence to Paul B. Brown.

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Bharadwaj, A.S., Hart, S.D., Brown, B.J. et al. Dietary Source of Stearidonic Acid Promotes Higher Muscle DHA Concentrations than Linolenic Acid in Hybrid Striped Bass. Lipids 45, 21–27 (2010). https://doi.org/10.1007/s11745-009-3372-9

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  • DOI: https://doi.org/10.1007/s11745-009-3372-9

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