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Effect of trans8, cis10+cis9, trans11 Conjugated Linoleic Acid Mixture on Lipid Metabolism in 3T3-L1 Cells

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Lipids

Abstract

Evidence suggests that minor isomers of conjugated linoleic acid (CLA), such as trans8, cis10 CLA, can elicit unique biological effects of their own. In order to determine the effect of a mixture of t8, c10+c9, t11 CLA isomers on selected aspects of lipid metabolism, 3T3-L1 preadipocytes were differentiated for 8 days in the presence of 100 μM linoleic acid (LA); t8, c10+c9, t11 CLA; t10, c12+c9, t11 CLA or purified c9, t11 CLA. Whereas supplementation with c9, t11 and t10, c12+c9, t11 CLA resulted in cellular triglyceride (TG) concentrations of 3.4 ± 0.26 and 1.3 ± 0.11 μg TG/μg protein, respectively (P < 0.05), TG accumulation following treatment with CLA mixture t8, c10+c9, t11 was significantly intermediate (2.5 ± 0.22 μg TG/μg protein, P < 0.05) between the two other CLA treatments. However, these effects were not attributable to an alteration of the Δ9 desaturation index. Adiponectin content of adipocytes treated with t8, c10+c9, t11 mixture was similar to the individual isomer c9, t11 CLA, and both the t8, c10+c9, t11 and c9, t11 CLA groups were greater (P < 0.05) than in the t10, c12+c9, t11 CLA group. Overall, these results suggest that t8, c10+c9, t11 CLA mixture affects TG accumulation in 3T3-L1 cells differently from the c9, t11 and t10, c12 isomers. Furthermore, the reductions in TG accumulation occur without adversely affecting the adiponectin content of these cells.

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Abbreviations

BSA:

Bovine serum albumin

CLA:

Conjugated linoleic acid

DMEM:

Dulbecco’s Modified Eagle’s Medium

FAME:

Fatty acid methyl ester

FFA:

Free fatty acid

GC-FID:

Gas chromatograph(y)-flame ionisation detector

LA:

Linoleic acid

MUFA:

Monounsaturated fatty acid

NL:

Neutral lipid

SCD:

Stearoyl CoA desaturase

TG:

Triglyceride

TLC:

Thin layer chromatography

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Acknowledgment

This study was conducted with the support of the Advanced Foods and Materials Network, as part of the Networks of Centres of Excellence, Canada.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Institute of Nutraceuticals and Functional Foods, Laval University, 2425 Agriculture St., Paul-Comtois Building, Quebec, QC, G1V 0A6, Canada

    Shama V. Joseph & Hélène Jacques

  2. Department of Biochemistry and Molecular Biology, Dalhousie University, 5850 College Street, Room 9C Sir Charles Tupper Medical Building, Halifax, NS, B3H 1X5, Canada

    Jessica R. Miller & Roger S. McLeod

Authors
  1. Shama V. Joseph
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  2. Jessica R. Miller
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  3. Roger S. McLeod
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  4. Hélène Jacques
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Correspondence to Hélène Jacques.

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Joseph, S.V., Miller, J.R., McLeod, R.S. et al. Effect of trans8, cis10+cis9, trans11 Conjugated Linoleic Acid Mixture on Lipid Metabolism in 3T3-L1 Cells. Lipids 44, 613–620 (2009). https://doi.org/10.1007/s11745-009-3309-3

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  • DOI: https://doi.org/10.1007/s11745-009-3309-3

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