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Lipids

, Volume 44, Issue 5, pp 425–435 | Cite as

Lysophosphatidylcholine Exhibits Selective Cytotoxicity, Accompanied by ROS Formation, in RAW 264.7 Macrophages

  • Cheon Ho Park
  • Mee Ree Kim
  • Jong-Min Han
  • Tae-Sook Jeong
  • Dai-Eun Sok
Original Article

Abstract

Lysophosphatidylcholine (lysoPtdCho) is a component of oxidized low density lipoprotein, and is involved in the pathogenesis of atherosclerosis and inflammation. We studied the effects of lysoPtdCho on cytotoxicity, reactive oxygen species (ROS) production, activation of the extracellular signal-regulated kinase (ERK), mitogen-activated protein kinases and pro-inflammatory gene expression in RAW 264.7 murine macrophage cells. When cells were exposed to lysoPtdCho with various acyl chains in a culture medium containing 10% fetal bovine serum, only 1-linoleoyl (C18:2) lysoPtdCho showed a remarkable cytotoxicity, reaching the highest level at 24 h, and elicited ROS production, suggesting that oxidative stress might be implicated in the cytotoxicity of 1-linoleoyl (C18:2) lysoPtdCho. Presumably in support of this, antioxidants such as magnolol or trolox prevented 1-linoleoyl (C18:2) lysoPtdCho-induced cytotoxicity as well as ROS production, although only partially. Furthermore, the phosphorylation of ERK 1/2 and the expression of pro-inflammatory cytokines such as IL-1β, CCL2 and CCL5 were augmented by 1-linoleoyl (C18:2) lysoPtdCho. Meanwhile, there was no structural importance of the acyl chain for the cytotoxic action of lysoPtdCho during 10 min incubation in serum-free media. Taken together, it is suggested that in a serum-containing medium, 1-linoleoyl (C18:2) lysoPtdCho can cause a significant cytotoxicity through ROS production, probably accompanied by activation of ERK and induction of related inflammatory cytokines, in RAW 264.7 cells.

Keywords

Delayed cytotoxicity Lysophosphatidylcholine Macrophage Inflammation NADPH oxidase Atherosclerosis Cytokines Extracellular signal-regulated kinase Antioxidants 

Abbreviations

lysoPtdCho

Lysophosphatidylcholine

ROS

Reactive oxygen species

FBS

Fetal bovine serum

MTT

3-(4,5-Dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide

ERK

Extracellular signal-regulated kinase

CCL

Chemotactic cytokines ligand

Notes

Acknowledgment

This work was financially supported by a Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-531-C00067), Korea.

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Copyright information

© AOCS 2009

Authors and Affiliations

  • Cheon Ho Park
    • 1
  • Mee Ree Kim
    • 2
  • Jong-Min Han
    • 3
  • Tae-Sook Jeong
    • 3
  • Dai-Eun Sok
    • 1
  1. 1.College of PharmacyChungnam National UniversityTaejonRepublic of Korea
  2. 2.Department of Food and NutritionChungnam National UniversityTaejonRepublic of Korea
  3. 3.National Research Laboratory of Lipid Metabolism and AtherosclerosisKRIBBTaejonRepublic of Korea

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