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Lipid and Phospholipid Profiling of Biological Samples Using MALDI Fourier Transform Mass Spectrometry

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Lipids

Abstract

Here we describe a study of the feasibility of lipid and phospholipid (PL) profiling using matrix assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) for two different applications. In this work PL profiles of different mammalian tissues as well as those of whole cell organisms were examined. In particular, comparative analysis of lipid and PL profiles of tissues from mice fed different diets was done and, in another application, MALDI FTMS was used to analyze PL profiles of genetically modified Saccharomyces cerevisiae. Computational sorting of the observed ions was done in order to group the lipid and PL ions from complex MALDI spectra. The PL profiles of liver tissues from mice fed different diets showed a cross correlation coefficient of 0.2580, indicating significant dissimilarity, and revealed more than 30 significantly different peaks at the 99.9% confidence level. Histogram plots derived from the spectra of wild type and genetically modified yeast resulted in a cross correlation coefficient 0.8941 showing greater similarity, but still revealing a number of significantly different peaks. Based on these results, it appears possible to use MALDI FTMS to identify PLs as potential biomarkers for metabolic processes in whole cells and tissues.

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Acknowledgments

The authors are grateful for financial support from the Arkansas Biosciences Institute and NSF grants CHE-00-91868 and CHE-99-82045. P.K. acknowledges support from NIH-GM-66164. The authors appreciate discussions with R. M. O’Brien and support from the Arkansas Statewide Mass Spectrometry Facility.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, USA

    S. Mariccor A. B. Batoy, Josephine Griffith & Charles L. Wilkins

  2. Institute for Analytical Science (ISAS), Bunsen-Kirchhoff-Strasse 11, 44139, Dortmund, Germany

    Sabine Borgmann

  3. Applied Proteomics Inc., Glendale, CA, USA

    Jeffrey J. Jones

  4. Department of Biological Chemistry, University of California, Irvine, CA, USA

    Karin Flick & Peter Kaiser

  5. Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA

    Viswanathan Saraswathi & Alyssa H. Hasty

Authors
  1. S. Mariccor A. B. Batoy
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  2. Sabine Borgmann
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  3. Karin Flick
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  4. Josephine Griffith
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  5. Jeffrey J. Jones
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  6. Viswanathan Saraswathi
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  7. Alyssa H. Hasty
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  8. Peter Kaiser
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  9. Charles L. Wilkins
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Corresponding author

Correspondence to Charles L. Wilkins.

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Batoy, S.M.A.B., Borgmann, S., Flick, K. et al. Lipid and Phospholipid Profiling of Biological Samples Using MALDI Fourier Transform Mass Spectrometry. Lipids 44, 367–371 (2009). https://doi.org/10.1007/s11745-008-3260-8

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  • DOI: https://doi.org/10.1007/s11745-008-3260-8

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