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Spin-Probe Investigations of Head Group Behavior in Aqueous Dispersions of a Nonionic Amphiphilic Compound

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Lipids

Abstract

Head group behavior of nonionic amphiphilic compound, (poly(oxyethylene) hydrogenated castor oil, HCO), in aqueous dispersions were investigated by EPR (electron paramagnetic resonance) in conjunction with a modern slow-tumbling simulation. The aliphatic spin probes, 5-doxylstearic acid (5-DSA) and 3β-doxyl-5α-cholestane (CHL), were used to obtain fluidity of the surface region of the membrane. The order parameter (S 0) using the simulation for 5-DSA and CHL in the region were approximately 0.4 and 0.2, respectively. The ordering results suggest that the head group region of the membrane is somewhat fluid. The rotational diffusion coefficients (R ⊥ ≈ 1/(6τR)) for the probes were 3.4 × 107 and 7.1 × 107 s−1, respectively. Activation energies, calculated using the temperature dependence of diffusion coefficients, were 18 and 17 kJ/mol for the probes. The EPR results imply that the CHL probe in the HCO membrane has quite different behavior in comparison with that of PC (phosphatidylcholine) from egg. Thus, the present EPR analyses have provided quantitative insight into the surface region of the amphiphilic membrane.

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Acknowledgments

The author thanks E. Yagi of Shiseido Research Center for useful suggestions of the sample preparations and Dr. Mingtao Ge of Cornell University for helpful discussions concerning the simulation. The latest version of the NLLS program was provided by Cornell University. Part of this research was supported by a Grant-in-Aid for Scientific Research (C) (18500347) from JSPS.

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  1. RI Research Center, Fukushima Medical University, 1 Hikarigaoka, Fukushima, 960-1295, Japan

    Kouichi Nakagawa

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  1. Kouichi Nakagawa
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Correspondence to Kouichi Nakagawa.

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Nakagawa, K. Spin-Probe Investigations of Head Group Behavior in Aqueous Dispersions of a Nonionic Amphiphilic Compound. Lipids 42, 457–462 (2007). https://doi.org/10.1007/s11745-007-3048-2

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  • DOI: https://doi.org/10.1007/s11745-007-3048-2

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