Rapid Analysis of Acylglycerols in Low Molecular Weight Milk Fat Fractions

Abstract

A suitable analytical method was required to facilitate development of an industrial-scale short-path distillation (SPD) process. Short-path distillation produces milk fat distillates (MFD) enriched in low molecular weight milk fat components—viz. free fatty acids, monoacylglycerols, diacylglycerols, cholesterol and low molecular weight triacylglycerols. In this case, solid-phase extraction (SPE) was considered a better alternative than thin-layer chromatography for separating polar and apolar lipid components in MFD samples due to its speed and near-complete recoveries. Solid-phase extraction of MFDs yielded two fractions, both of which are sufficiently pure for subsequent analysis by gas chromatography. This procedure provided rapid and complete chemical characterization (including mass balances) of low-molecular weight milk-fat fractions.

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Abbreviations

DAG:

Diacylglycerol

FA:

Fatty acid

FFA:

Free fatty acid

FID:

Flame ionization detector

GC:

Gas chromatograph(y)

MAG:

Monoacylglycerol

MF:

Milk fat

MFD:

Milk fat distillate

MFD170:

MFD collected at 170 °C

MFD185:

MFD collected at 185 °C

MFD195:

MFD collected at 195 °C

MFD200:

MFD collected at 200 °C

MW:

Molecular weight

RT:

Retention time

SE:

Standard error

SP:

Short-path

SPD:

Short-path distillation

SPE:

Solid-phase extraction

SPE-1:

First SPE fraction

SPE-2:

Second SPE fraction

TAG:

Triacylglycerol

TLC:

Thin-layer chromatography

TMS:

Trimethyl silyl

TMSI:

Trimethyl silyl imidazole

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Acknowledgments

We gratefully acknowledge the financial support of the Dairy Farmers of Ontario, Ontario Ministry of Agriculture, Food and Rural Affairs, Ontario Ministry of Training, Colleges and Universities, and National Sciences and Engineering Research Council of Canada.

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Correspondence to R. W. Lencki.

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Craven, R.J., Lencki, R.W. Rapid Analysis of Acylglycerols in Low Molecular Weight Milk Fat Fractions. Lipids 42, 473–482 (2007). https://doi.org/10.1007/s11745-007-3044-6

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Keywords

  • Minor lipids
  • Milk fat
  • Short-path distillation
  • Diacylglycerol
  • Gas chromatography