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Relationship Between Apolipoprotein Concentrations and HDL Subclasses Distribution

  • Original Article
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Lipids

Abstract

Alterations in plasma apolipoproteins levels can influence the composition, content, and distribution of plasma lipoproteins that affect the risk of atherosclerosis. This study assessed the relationship between plasma apolipoproteins levels, mainly apoAI, and HDL subclass distribution. The contents of plasma HDL subclasses were determined by two-dimensional gel electrophoresis coupled with immunodetection in 545 Chinese subjects. Compared with a low apoAI group, the contents of all HDL subclasses increased significantly both in middle and high apoAI group, and the contents of large-sized HDL2b increased more significantly relative to those of small-sized preβ1-HDL in a high apoAI group. When apoAI and HDL-C levels increased simultaneously, in comparison to a low apoAI along with HDL-C concentration group, a significant increase (116%) was shown in HDL2b but only a slight increase (26%) in preβ1-HDL. In addition, Pearson correlation analysis revealed that apoAI levels were positively and significantly correlated with all HDL subclasses. Multiple liner regression demonstrated that the apoAI concentrations were the most powerful predictor for HDL subclass distribution. With the elevation of apoAI concentrations, the contents of all HDL subclasses increased successively and significantly, especially, an increase in large-sized HDL2b. Further, when apoAI and HDL-C concentrations increased simultaneously, the shift to larger HDL size was more obvious. Which, in turn, indicated that HDL maturation might be enhanced and, the reverse cholesterol transport might be strengthened along with apoAI levels which might be a more powerful factor influencing the distribution of HDL subclasses.

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Acknowledgments

We thank technician Yu Liu, master students Jia Yao and Xuemei Zhang for the collection of blood sample and technical support in the lipid and apolipoprotein assays.

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Authors and Affiliations

  1. Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, New building 6, Room 902, #16 Section 3, People South Road, Chengdu, 610041, Sichuan, People’s Republic of China

    Li Tian, Mingde Fu, Lianqun Jia & Haoming Tian

  2. Chengdu Hoist Biotechnology Co., LTD, Sichuan, People’s Republic of China

    Yanhua Xu

  3. Department of Biochemistry and Molecular Biology, Nanhua University, Hengyang, Hunan, People’s Republic of China

    Shiyin Long & Ying Tian

Authors
  1. Li Tian
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  2. Mingde Fu
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  3. Lianqun Jia
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  4. Yanhua Xu
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  5. Shiyin Long
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  6. Haoming Tian
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  7. Ying Tian
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Correspondence to Mingde Fu.

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Tian, L., Fu, M., Jia, L. et al. Relationship Between Apolipoprotein Concentrations and HDL Subclasses Distribution. Lipids 42, 419–426 (2007). https://doi.org/10.1007/s11745-007-3031-y

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  • DOI: https://doi.org/10.1007/s11745-007-3031-y

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