Skip to main content
SpringerLink
Log in

Differential biohydrogenation and isomerization of [U-13C]Oleic and [1-13C]Oleic acids by mixed ruminal microbes

  • Communications
  • Published:
Lipids

Abstract

The additional mass associated with 13C in metabolic tracers may interfere with their metabolism. The comparative isomerization and biohydrogenation of oleic, [1-13C]oleic, and [U-13C]oleic acids by mixed ruminal microbes was used to evaluate this effect. The percent of stearic, cis-14 and- 15, and trans-9 to-16 18∶1 originating from oleic acid was decreased for [U-13C]oleic acid compared with [1-13C]oleic acid. Conversely, microbial utilization of [U-13C]oleic acid resulted in more of the 13C label in cis-9 18∶1 compared with [1-13C]oleic acid (53.7 vs. 40.1%). The isomerization and biohydrogenation of oleic acid by ruminal microbes is affected by the mass of the labeled tracer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

DMDS:

dimethyl disulfide

E:

enrichment

TTR:

tracer to tracee ratio

References

  1. Wolfe, R.R. (1992) Radioactive and Stable Isotope Tracers in Biomedicine, Principles and Practice of Kinetic Analysis, Wiley-Liss, New York.

    Google Scholar 

  2. Koletzko, B., Sauerwald, T., and Demmelmair, H. (1997) Safety of Stable Isotope Use. Eur. J. Pediatr. 156, S12-S17.

    Article  PubMed  CAS  Google Scholar 

  3. Hachey, D.L., Thomas, M.R., Emken, E.A., Garza, C., Brown-Booth, L., Adlof, R.O., and Klein, P.D (1987) Human Lactation: Maternal Transfer of Dietary Triglycerides Labeled with Stable Isotopes, J. Lipid Res. 28, 1185–1192.

    PubMed  CAS  Google Scholar 

  4. Demmelmair, H., Baumheuer, M., Koletxko, B., Dokoupil, K., and Kratl, G. (1998) Metabolism of U-13C-Labeled Linoleic Acid in Lactating Women. J. Lipid Res. 39, 1389–1396.

    PubMed  CAS  Google Scholar 

  5. Bell, P.M, Firth, R.G., and Rizza, R.A. (1986) Assessment of Insulin Action in Insulin-Dependent Diabetes Mellitus using [6-14C]Glucose, [3-3H]Glucose, and [2-3H]Glucose: Differences in the Apparent Pattern of Insulin Resistance Depending on the Isotope Used. J. Clin. Invest. 78, 1479–1486.

    Article  PubMed  CAS  Google Scholar 

  6. Argoud, G.M., Schade, D.S., and Eaton, R.P. (1987) Underestimation of Hepatic Glucose Production by Radioactive and Stable Tracers, Am. J. Physiol. 252, E606-E615.

    PubMed  CAS  Google Scholar 

  7. Chatzidakis, C., and Otto, D.A. (1987) Labeled Oxidation Products from [1-14C], [1-14C], and [16-14C]-Palmitate in Hepatocytes and Mitochondria, Lipids 22, 620–626.

    Article  PubMed  CAS  Google Scholar 

  8. Mosley, E.E., Powell, G.L., Riley, M.B., and Jenkins, T.C. (2002) Microbial Biohydrogenation of Oleic Acid to trans Isomers in vitro, J Lipid. Res. 43, 290–296.

    PubMed  CAS  Google Scholar 

  9. Harfoot, C.G., and Hazlewood, G.P. (1988) Lipid Metabolism in the Rumen, in The Rumen Microbial Ecosystem ed. P. N. Hobson, pp. 285–322. Elsevier Applied Science Publishers. London, [AUQ]

    Google Scholar 

  10. Jenkins, T.C. (1993) Lipid Metabolism in the Rumen. J Dairy Sci. 76, 3851–3863. [AUQ]

    Article  PubMed  CAS  Google Scholar 

  11. Kramer, J.K.G., Fellner, V., Dugan, M.E.R., Sauer, F.D., Mossoba, M.M., and Yurawecz, M.P. (1997) Evaluating Acid and Base Catalysts in the Methylation of Milk and Rumen Fatty Acids with Special Emphasis on Conjugated Dienes and Total trans Fatty Acids, Lipids 32, 1219–1228.

    Article  PubMed  CAS  Google Scholar 

  12. Mosley, E.E., Shafii, B., Moate, P.J., and McGuire, M.A. (2006) Conjugated Linoleic Acid (cis-9, trans-11 CLA) Is Synthesized Directly from Vaccenic Acid in Lactating Dairy Cattle. J. Nutr. 136, 570–575.

    PubMed  CAS  Google Scholar 

  13. Proell, J.M., Mosley, E.E., Powell, G.L., and Jenkins, T.C. (2002) Isomerization of Stable Isotopically Labeled Elaidic Acid to cis and trans Monoenes by Ruminal Microbes. J. Lipid Res. 43, 2072–2076.

    Article  PubMed  CAS  Google Scholar 

  14. Palmquist, D.L., and Mattos, W. (1978) Turnover of Lipoproteins and Transfer to Milk Fat of Dietary (1-Carbon-14) Linoleic Acid in Lactating Cows, J. Dairy Sci. 61, 561–565.

    CAS  Google Scholar 

  15. Lauryssens, M., Verbeke, R., and Peeters, G. (1961) Metabolism of Stearate-1-C14 in the Isolated Cow's Udder, J. Lipid Res. 2, 383–388.

    CAS  Google Scholar 

  16. Emken, E.A., Adlof, R.O., Hachey, D.L., Garza, C., Thomas, M.R., and Brown-Booth, L. (1989) Incorporation of Deuterium-Labeled Fatty Acids into Human Milk Plasma, and Lipoprotein Phospholipids and Cholesteryl Esters, J. Lipid Res. 30, 395–402.

    PubMed  CAS  Google Scholar 

  17. Emken, E.A., Adlof, R.O., Duval, S.M., Shane, J.M., Waler, P. M., and Becker, C. (2004) Effect of Triacylglycerol Structure on Absorption and Metabolism of Isotope-Labeled Palmitic and Linoleic Acids by Humans. Lipids 39, 1–9.

    Article  PubMed  CAS  Google Scholar 

  18. Bosner, M.S., Ostlund, Jr, R.E., Osofisan O., Grosklos, J., Fritschle, C., and Lange, L.G. (1993) Assessment of Percent Cholesterol Absorption in Humans with Stable Isotopes. J. Lipid Res. 34, 1047–1053.

    PubMed  CAS  Google Scholar 

  19. Lin, Y.H., Pawlosky, R.J., and Salem, Jr, N. (2005) Simultaneous Quantitative Determination of Deuterium- and Carbon-13-Labeled Essential Fatty Acids in Rat Plasma. J. Lipid Res. 46, 1974–1982.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Animal and Veterinary Science, University of Idaho, PO Box 442330, Moscow, 83844-2330, Moscow, Idaho

    Erin E. Mosley & Mark A. McGuire

  2. Dipartimento di Scienze Zootecniche, Università di Sassari, Via Enrico De Nicola, 9-07100, Sassari, Italy

    Anna Nudda

  3. Department of Animal Science, University of Padova, Viale dell'Università, 161-35020, Legnaro (PD), Italy

    Adolfo Corato & Erica Rossi

  4. Department of Animal and Veterinary Sciences, Clemson University, 29634, Clemson, South Carolina

    Tom Jenkins

Authors
  1. Erin E. Mosley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anna Nudda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Adolfo Corato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Erica Rossi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tom Jenkins
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mark A. McGuire
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mark A. McGuire.

About this article

Cite this article

Mosley, E.E., Nudda, A., Corato, A. et al. Differential biohydrogenation and isomerization of [U-13C]Oleic and [1-13C]Oleic acids by mixed ruminal microbes. Lipids 41, 513–517 (2006). https://doi.org/10.1007/s11745-006-5125-3

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11745-006-5125-3

Keywords

Search

Navigation