Abstract
Our aim was to compare the effects of two pelleted diets containing differing FA composition with natural lichen pasture on reindeer's meat FA composition. In addition we wanted to increase the knowledge about reindeer FA metabolism and the effect of animal sex and age on FA composition in reindeer muscle.
The trial included five reindeer groups: three grazing, consisting of adult males, adult females, and calves; and two groups of calves fed conventional pellets (CPD) and pellets containing crushed linseed (LPD), respectively, for two months before slaughter. Differences between male and female animals were mainly found in the neutral lipid fraction and related to fatness. Calves differed significantly from adult males and females in FA and lipid class composition. CPD led to a higher ratio of n−6 to n−3 FA compared with grazing. The ratio n−6/n−3 in the polar lipid (PL) fraction of the animals fed LPD was slightly, but not significantly, higher than that in the grazing reindeer. LPD-fed animals had lower proportions of long-chain polyunsaturated FA (LCPUFA), namely 20∶4n−6, 22∶5n−3, and 22∶6n−3, in the PL fraction compared with the grazing animals due to the content of these FA in the natural feed. The animals seemed unable to elongate dietary FA in significant amounts. We conclude that by adding crushed linseed to the pellets it was possible to keep the favorable FA composition of meat from grazing reindeer with regard to the n−6/n−3 ratio but not in LCPUFA.
Similar content being viewed by others
Abbreviations
- CPD:
-
conventional pellets
- GLM:
-
general linear model
- HIP:
-
hexan-isopropanol
- LCPUFA:
-
long-chain polyunsaturated FA
- LPD:
-
linseed pellets
- MUFA:
-
monounsaturated FA
- NL:
-
neutral lipid fraction
- PL:
-
polar lipid fraction
- SFA:
-
saturated FA
- SPE:
-
solid phase extraction
References
Williams, C.M. (2000) Dietary Fatty Acids and Human Health, Ann. Zootech. 49, 165–180.
Simopoulos, A.P. (2001) Evolutionary Aspects of Diet and Essential Fatty Acids, in Fatty Acids and Lipids: New Findings, Hamazaki, T., and Okuyama, H., eds., vol. 88. pp. 18–27, Basel, Switzerland, Karger.
Simopoulos, A.P. (2002) The Importance of the Ratio of Omega-6/Omega-3 Essential Fatty Acids, Biomed. Pharmacother. 56, 365–379.
Wood J.D., Richardson, R.I., Nute, G.R., Fisher, A.V., Campo, M.M., Kasapidou, E., Sheard, P.R., and Enser, M. (2003) Effects of Fatty Acids on Meat Quality: A Review, Meat Sci. 66, 21–32.
Wiklund, E., Pickova, J., Sampels, S., and Lundström, K. (2001) Fatty Acid Composition of M. longissimus lumborum, Ultimate Muscle pH Values and Carcass Parameters in Reindeer (Rangifer tarandus tarandus L.) Grazed on Natural Pasture or Fed a Commercial Feed Mixture, Meat Sci. 58, 293–298.
Skjenneberg, S., and Slagsvold, L. (1968) Reindriften og dens naturgrunnlag, [in Norwegian], Oslo/Bergen/Tromsø: Scandinavian University Books—Universitetsförlaget.
Nieminen, M., and Heiskari, U. (1989) Diets of Freely Grazing and Captive Reindeer During Summer and Winter, Rangifer 9, 17–34.
Åhman, B. (1999) Transfer of Radiocaesium via Reindeer Meat to Man: Effects of Countermeasures Applied in Sweden Following the Chernobyl Accident, J. Environ. Radioact. 46, 113–120.
Jacobsen, E., Bjarghov, R.S., and Skjenneberg, S. (1977) Nutritional Effect on Weight Gain and Winter Survival of Reindeer Calves (Rangifer tarandus tarandus), Meldinger fra norges lantbrukshøgskole (Scientific Reports of the Agricultural University of Norway) 56, 1–12.
Reimers, E. (1983) Growth Rate and Body Size Differences in Rangifer, a Study of Causes and Effects, Rangifer 3, 3–15.
Taugbøl, O., and Mathiesen, S. D. (1999) Nutrient Dependent Composition of Polyunsaturated Fatty Acids in M. Psoas Minor from Reindeer, in Proceedings of the 10th Arctic Ungulate Conference, Tromsø, Norway, p. 59.
Rule, D.C., Smith, S.B. and Romans, J.R. (1995) Fatty Acid Composition of Muscle and Adipose Tissue of Meat Animals. in Biology of Fat in Meat Animals Current Advances, Smith, S.B., and Smith, D.R., pp. 144–165, Champaign: American Society of Animal Science.
Demeyer, D., and Doreau, M. (1999) Targets and Procedures for Altering Ruminant Meat and Milk Lipids, Proc. Nutr. Soc., 58, 593–607.
Wood, J.D., and Enser, M. (1997) Factors Influencing Fatty Acids in Meat and the Role of Antioxidants in Improving Meat Quality, Br. J. Nutr. 78, S49-S60.
Scollan, N.D., Choi, N.-J., Kurt, E., Fisher, A.V., Enser, M., and Wood, J.D. (2001) Manipulating the Fatty Acid Composition of Muscle and Adipose Tissue in Beef Cattle, Br. J. Nutr. 85, 115–124.
Matsuoka, A., Furokawa, N., and Takahashi, T. (1997) Carcass Traits and Chemical Composition of Meat in Male and Female Goats, J. Agric. Sci. Tokyo Nogyo Daigaki 42, 127–135.
Wood, J.D., and Enser, M. (1988) Effects of Carcass Fatness and Sex on the Composition and Quality of Pig Meat, in Proceedings 34th International Congress of Meat Science and Technology, Brisbane, Australia, pp. 7–10.
Huerta-Leidenz, N.O., Cross, H.R., Savell, J.W., Lunt, D.K., Baker, J.F., and Smith, S.B. (1996) Fatty Acid Composition of Subcutaneous Adipose Tissue from Male Calves at Different Stages of Growth, J. Anim. Sci. 74, 1256–1264.
Högberg, A., Pickova, J., Dutta, P.C., Babol, J., and Bylund, A.C. (2001) Effect of Rearing System on Muscle Lipids of Gilts and Castrated Male Pigs, Meat Sci. 58, 223–229.
Sampels, S., Pickova, J., and Wiklund, E. (2004) Fatty Acids, Antioxidants and Oxidation Stability of Processed Reindeer Meat, Meat Sci. 67, 523–532.
Svenska Lantmännen. (2003) Renfor näringsdeklaration. [Renfor declaration of ingredients; in Swedish], http://www.lantmannen.se/default.aspx?id=1 (accessed Jan 2003).
Sampels, S., Pickova, J., and Wiklund E. (2005) Influence of Production System, Age and Sex on Carcass Parameters and Some Biochemical Meat Quality Characteristics of Reindeer (Rangifer tarandus tarandus L.), Rangifer 25, 85–96.
Hara, A., and Radin, N.S. (1978) Lipid Extraction of Tissues with Low Toxicity Solvent, Anal. Biochem. 90, 420–426.
Pickova, J., Dutta, P.C., Larsson, P.O., and Kiessling, A. (1997) Early Embryonic Cleavage Pattern, Hatching Success, and Egg Lipid Fatty Acid Composition: Comparison Between Two Cod (Gadus morhua) Stocks, Can. J. Fish. Aquat. Sci. 54, 2410–2416.
Folch, J., Lees, M., and Sloane Stanley, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem. 226, 497–509.
Prieto, J.A., Ebri, A., and Collar, C. (1992) Optimized Separation of Nonpolar and Polar Lipid Classes from Wheat-Flour by Solid-Phase Extraction, J. Am. Oil Chem. Soc. 69, 387–391.
Appelqvist, L.-Å. (1968) Rapid Methods of Lipid Extraction and Fatty Acid Methyl Ester Preparation for Seed and Leaf Tissue with Special Remarks on Preventing the Accumulation of Lipid Contaminants, Royal Swedish Academy of Science (Kungliga Svenska Vetenskapsakademien) Stockholm 28, 551–570.
Högberg, A. (2002) Fatty Acids, Tocopherols and Lipid Oxidation in Pig Muscle: Effects of Feed, Sex and Outdoor Rearing, Ph.D. Thesis Swedish University of Agricultural Sciences, Department of Food Science, Uppsala, Sweden, p. 40.
Jensen, K., Jensen, C., Jakbbsen, K., Engberg, R.M., Andersen, J.O., Lauridsen, C., Sorensen, P., Skibsted, L.H., and Bertelsen, B. (1998) Supplementation of Broiler Diets with Retinol Acetate, Beta-Carotene or Canthaxanthin: Effect on Vitamin Status and Oxidative Status of Broilers in vivo and on Meat Stability, Acta Anaesth. Scand. 48, 28–37.
SAS Institute (1997) SAS System for Windows, release 6.12, Version 8.02. SAS Institute Inc., Cary, NC.
Storeheier, P.V., Mathiesen, S.D., Tyler, N.J.C., and Olsen, M.A. (2002) Nutritive Value of Terricolous Lichens for Reindeer in Winter, Lichenologist 34, 247–257.
Soppela, P., Heiskari, U., Nieminen, M., Salminen, I., Sankari S., and Kindahl, H. (2000) The Effects of a Prolonged Undernutrition on Serum Lipids and Fatty Acid Composition of Reindeer Calves During Winter and Spring, Acta Physiol. Scand. 168, 337–350.
Malau-Aduli, A.E., Siebert, B.D., Bottema, C.D., and Pitchford, W.S. (1998) Breed Comparison of the Fatty Acid Composition of Muscle Phospholipids in Jersey and Limousin Cattle, J. Anim. Sci. 76, 766–773.
Prior, R. (1983) Lipid Metabolism in Finishing Bulls and Steers Implanted with Oestradiol-17β-α:Dipropionate, Anim. Prod. 37, 81–85.
Nürnberg, K., Grumbach, S., Papstein, H.J., Matthes, H.D., Ender, K., and Nürnberg, G. (1996) Fatty Acid Composition of Lamb Meat, Fett/Lipid 98, 77–80
Nürnberg, K., Wegner, J., and Ender, K. (1998) Factors Influencing Fat Composition in Muscle and Adipose Tissue of Farm Animals, Livest. Prod. Sci. 56, 145–156.
Staaland, H., and Sæbø, S. (1987) Seasonal Variations in Mineral Status of Reindeer Calves from Elgaa Reindeer Herding District, Norway, Rangifer 7, 22–28.
Gaare, E., and Skogland, T. (1975) Wild Reindeer Food Habits and Range Use at Hardangervidda, Ecol. Stud. 17, 195–205.
Mathiesen, S.D., Haga, O.E., Kaino, T., and Tyler, N.J.C. (2000) Diet Composition, Rumen Papillation and Maintenance of Carcass Mass in Female Norwegian Reindeer (Rangifer tarandus tarandus) in Winter, J. Zool., 251, 129–138.
Kumpula, J., (2001) Vinter Grazing of Reindeer in Woodland Lichen Pasture: Effect of Lichen Availability on the Condition of Reindeer, Small Ruminant Res. 39, 121–130.
Elgersma, A., Ellen, G., van der Horst, H., Muuse, B.G., Boer, H., and Tamminga, S. (2003) Comparison of the Fatty Acid Composition of Fresh and Ensiled Perennial Ryegrass (Lolium perenne L.), Affected by Cultivar and Regrowth Interval, Anim. Feed Sci. Technol. 108, 191–205.
Ratnayake, W.M.N., Ackman, R.G., and Hulan, H.W. (1989) Effect of Redfish Meal Enriched Diets on the Taste and n−3 PUFA of 42-Day-Old Broiler Chickens, J. Sci. Food Agric., 49, 59–74.
Rooke, J.A., Shanks, M., and Edwards, S.A. (2001) Effects of Offering Maize, Linseed or Tuna Oils Throughout Pregnancy and Lactation on Sow and Piglet Tissue Composition and Piglet Performance, Anim. Sci. 71, 289–299.
Arts, M.T., Ackman, R.G., and Holub, B.J. (2001) “Essential Fatty Acids” in Aquatic Ecosystems: A Crucial Link Between Diet and Human Health and Evolution, Can. J. Fish. Aquat. Sci. 58, 122–137.
Yang, A., Brewster, M.J., Lanari, M.C., and Tume, R.K. (2002) Effect of Vitamin E Supplementation on-Tocopherol and,-Carotene Concentrations in Tissues from Pasture-and Grain-Fed Cattle, Meat Sci. 60, 35–40.
Jensen, C., Lauridsen, C., and Bertelsen, G. (1998) Dietary Vitamin E: Quality and Storage Stability of Pork and Poultry, Trends Food Sci. Technol. 9, 62–72.
Frank, J. (2004) Dietary Phenolic Compounds and Vitamin E Bioavailability: Model Studies in Rats and Humans, Ph.D. Thesis, Swedish University of Agriculture, Department of Food Science, Uppsala, Sweden, p. 34.
Gülçin, I., Oktay, M., Küfreviolu, Ö.I., and Aslan, A. (2002) Determination of Antioxidant Activity of Lichen Ceraria islandica (L) Ach, J. Ethnopharmacol. 79, 325–329.
Laitinen, J., Näyhä, S., Sikkila, K., and Hassi, J. (1996) Diet and Cardiovascular Risk Factors Among Lapp and Finnish Reindeer Herders, Nutr. Res. 16, 1083–1093.
Nilsen, H., Utsi, E., and Bonaa, K.H. (1999) Dietary and Nutrient Intake of a Sami Population Living in Traditional Reindeer Herding Areas in North Norway: Comparisons with a Group of Norwegians, Int. J. Circumpolar Health 58, 120–133.
Näyhä, S. (1997) Low Mortality from Ischaemic Heart Disease in the Sámi District in Finland, Soc. Sci. Med. 44, 123–131.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Sampels, S., Wiklund, E. & Pickova, J. Influence of diet on fatty acids and tocopherols in M. Longissimus Dorsi from reindeer. Lipids 41, 463–472 (2006). https://doi.org/10.1007/s11745-006-5120-8
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11745-006-5120-8