Abstract
Hair lipid images, as visualized by argon sputter etching-scanning electron microscopy (ASE-SEM), reveal convex structures with a stitch pattern (SP) at the cell membrane complex (CMC) in the transverse hair plane. Based on interindividual variation, different features of the convex SP were classified into Types 0 to 4 with the corresponding scores 0 to 4. Observations using hair fibers collected from 27 Japanese females revealed significant positive correlations between the scores and the levels of exogenous lipids, which suggests that exogenous lipids internalized at the CMC predominantly constitute the convex SP. Intraindividual variation with different levels of exogenous lipids among hair fibers derived from individual females may be relevant to the uneven physicochemical properties of hair fibers on the scalp. Observations of 380 hair fibers collected from Japanese (Mongoloid), German and American (Caucasoid) females aged 3 to 77 yr demonstrated similar age-related changes in the lipid images, which represent an increase and then a decrease in levels of exogenous lipids with increasing age. This suggests that age-related changes in exogenous lipids are attributable to alterations in sebum excreted during aging and that this elicits age-related changes in physical parameters, which affect human hair texture.
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Abbreviations
- ASE:
-
argon sputter etching
- BC:
-
β-cholestanol
- CER/MS:
-
ceramide/N-myristoyl sphingosine
- CER/PDS:
-
ceramide/N-palmitoyl-dl-dihydrosphingosine
- CERs:
-
ceramides
- CH:
-
cholesterol
- CMC:
-
cell membrane complex
- CP:
-
circular pattern
- CS:
-
cholesterol sulfate
- FFAs:
-
free fatty acids
- SEM:
-
scanning electron microscopy
- SIM:
-
selected ion monitoring
- SP:
-
stitch pattern
- SQ:
-
squalene
- TGs:
-
triglycerides
- WEs:
-
wax esters
References
Robbins, C.R. (1994) Chemical and Physical Behavior of Human Hair, 3rd edn., pp. 23–45, Springer-Verlag, New York.
Swift, J.A. (1967) The Electron Histochemistry of Cystine-Containing Proteins in Thin Transverse Sections of Human Hair. J. R. Microsc. Soc. 14, 449–460.
Wolfram, L.J., and Lindemann, M.K.O. (1971) Some Observations on the Hair Cuticle. J. Cosmet. Chem. 22, 839–850.
Kaplin, I.J., Schwan, A., and Zahn, H. (1982) Effects of Cosmetic Treatments on the Ultrastructure of Hair. Cosmet. Toiletries 97, 22–26.
Maruyama, T., Kanbe, T., and Torii, K. (1993) Investigation of the Human Hair Treated with the Ultrasonic Generator Using Transmission Electric Microscope. J. Soc. Cosmet. Chem. Jpn. 27, 144–151.
Menkart, J., Wolfram, L.J., and Mao, I. (1966) Caucasian Hair, Negro Hair, and Wool: Similarities and Differences. J. Cosmet. Chem. 17, 769–787.
Sakamoto, O., Fujinuma, Y., and Ozawa, T. (1977) Studies on the Chemical Composition of Internal Human Hair Lipid. J. Am. Oil Chem. Soc. 54, 143A.
Shaw, D.A. (1979) The Extraction. Quantification, and Nature of Hair Lipid, Int. J. Cosmet. Sci. 1, 291–302.
Koch, J., Aitzetmüller, K., Bittorf, G., and Waibel, J. (1982) Hair Lipids and Their Contribution to the Perception of Hair Oiliness, J. Soc. Cosmet. Chem. 33, 317–326.
Wertz, P.W., and Downing, D.T. (1988) Integral Lipids of Human Hair. Lipids 23, 878–881.
Hilterhaus-Bong, S., and Zahn, H. (1989) Contributions to the Chemistry of Human Hair: II. Lipid Chemical Aspects of Permanently Waved Hair. Int. J. Cosmet. Sci. 11, 167–174.
Hussler, G., Kaba, G., François, A.M., and Saint-Leger, D. (1995) Isolation and Identification of Human Hair Ceramides. Int. J. Cosmet. Sci. 17, 197–206.
Hoting, E., and Zimmermann, M. (1996) Photochemical Alterations in Human Hair. Part 3: Investigations of Internal Lipids, J. Soc. Cosmet. Chem. 47, 201–211.
Naito, S., Takahashi, T., Hattori, M., and Arai, K. (1992) Histochemical Observation of the Cell Membrane Complex of Hair, Sen-1 Gakkaishi 48, 420–426.
Sideris, V., Holt, L.A., and Leaver, I.H. (1990) A Microscopical Study of the Pathway for Diffusion of Rhodamine B and Octadecylrhodamine B into Wool Fibers, J. Soc. Dyers Colour. 106, 131–135.
Nishimura, K., Nishino, M., Inaoka, Y., Kitada, Y., and Fukushima, M. (1989) Interrelationship Between the Hair Lipids and the Hair Moisture, Nippon Koshohin Kagakkaishi 13, 134–139.
Philippe, M., Garson, J.C., Gilard, P., Hocquaux, M., Hussler, G., Leroy, F., Mahieu, C., Semeria, D., and Vanlerberghe, G. (1995) Synthesis of 2-N-Oleoylamino-octadecane-1,3-diol: A New Ceramide Highly Effective for the Treatment of Skin and Hair, Int. J. Cosmet. Sci. 17, 133–146.
Masukawa, Y., Tsujimura, H., and Imokawa, G. (2005) A Systematic Method for the Sensitive and Specific Determination of Hair Lipids in Combination with Chromatography, J. Chromatogr. B 823, 131–142.
Nicolaides, N., and Rothman, S. (1952) Studies on the Chemical Composition of Human Hair Fat, J. Invest. Dermatol. 19, 389–391.
Masukawa, Y., Narita, H., and Imokawa, G. (2005) Characterization of the Lipid Composition at the Proximal Root Regions of Human Hair. J. Cosmet. Sci. 56, 1–16.
Masukawa, Y., Shimogaki, H., Manago, K., and Imokawa, G. (2005) A Novel Method for Visualizing Hair Lipids at the Cell Membrane Complex: Argon Sputter Etching-Scanning Electron Microscopy. J. Cosmet. Sci. 56, 297–309.
Pochi, P.E., Strauss, J.S., and Downing, D.T. (1979) Age-Related Changes in Sebaceous Gland Activity, J. Invest. Dermatol. 73, 108–111.
Porro, M.N., Passi, S., Boniforti, L., and Belsito, F. (1979) Effects of Aging on Fatty Acids in Skin Surface Lipids, J. Invest. Dermatol. 73, 112–117.
Naruse, N., and Fujita, T. (1971) Changes in the Physical Properties of Human Hair with Age, J. Am. Geriatr. Soc. 19, 308–314.
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Masukawa, Y., Tanamachi, H., Tsujimura, H. et al. Characterization of hair lipid images by argon sputter etching-scanning electron microscopy. Lipids 41, 197–205 (2006). https://doi.org/10.1007/s11745-006-5088-4
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DOI: https://doi.org/10.1007/s11745-006-5088-4