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Dietary phospholipids are more efficient than neutral lipids for long-chain polyunsaturated fatty acid supply in European sea bass Dicentrarchus labrax larval development

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Lipids

Abstract

We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n−3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d post-hatch with five isoproteic and isolipidic compound diets with different levels of EPA and DHA. Diet names indicated the percentage of EPA and DHA contained in the phospholipids (PL) and neutral lipids (NL), as follows: PL5, PL3, PL1, NL1, and NL3. Histological observations showed different patterns of lipid absorption and accumulation in the intestinal mucosa depending on the level and nature of the dietary lipid fraction. Fish fed high levels of neutral lipids (11%, NL3 diet: 2.6% of EPA+DHA in the NL fraction) showed large intracellular and intercellular lipid deposits in the anterior intestine, but no such lipid accumulation was detected when larvae were fed with low and moderate levels of EPA and DHA in the phospholipid and neutral lipid fractions of the diet (PL and NL1 diets). PL were preferentially absorbed in the postvalvular intestine, and the accumulation of marine PL was inversely correlated to their dietary level. The postvalvular intestinal mucosa and liver showed signs of steatosis; large lipid vacuoles were observed in this region of the intestine and in the liver and were inversely correlated with the level of dietary neutral lipids. The best results in terms of growth, survival, and development (maturation of the digestive system and histological organization of the liver and intestinal mucosa) were obtained in the group fed with 2.3% of EPA and DHA in the PL fraction of the diet (PL3 diet), revealing that European sea bass larvae use the LC-PUFa contained in the PL fraction more efficiently than those from the NL fraction of the diet.

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Abbreviations

CCK:

cholecystokinin

dph:

days post-hatch

IFREMER:

L'Institut Francais de Recherche pour l'Exploitation de la Mer

INRA:

Institut National de la Recherche Agronomique

IS:

intestinal segment

microdiet:

microparticulated diet (particle size 200–400 μm

NL:

neutral lipids

PL:

phospholipids(s)

PS:

pancreatic segment

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Authors and Affiliations

  1. Unité Mixte de Nutrition des Poissons, L'Institut Francais de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut National de la Recherche Agronomique (INRA), IFREMER, 29280, Plouzané, France

    L. Villeneuve, J. L. Zambonino-Infante, P. Quazuguel & C. L. Cahu

  2. Centre d'Aqüicultura, Institut de Recerca i Tecnologia Agroalimentaries (IRTA), Aptat. Correaus 200, 43540, Sant Carles de la Ràpita, Tarragona, Spain

    E. Gisbert

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  1. E. Gisbert
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  2. L. Villeneuve
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  3. J. L. Zambonino-Infante
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  4. P. Quazuguel
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  5. C. L. Cahu
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Correspondence to E. Gisbert.

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Gisbert, E., Villeneuve, L., Zambonino-Infante, J.L. et al. Dietary phospholipids are more efficient than neutral lipids for long-chain polyunsaturated fatty acid supply in European sea bass Dicentrarchus labrax larval development. Lipids 40, 609–618 (2005). https://doi.org/10.1007/s11745-005-1422-0

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