Abstract
Active lipases were isolated from the culture supernatant of the basidiomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic activity of 95% was obtained at pH 7.0 and 60 mL N2 min−1 after 50 min. The procedure, with minor modifications, was also applicable to native pellet cultures of P. sapidus. The same recovery of 95% was achieved, with purification and enrichment factors of 11.6 and 28.0, respectively.
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Abbreviations
- DSM:
-
Deutsche Sammlung von Mikroorganismen und Zellkulturen
- E:
-
enrichment factor
- IEF:
-
isoelectric focusing
- P:
-
purification factor
- Ra:
-
recovery of activity
- Rp:
-
recovery of protein
- SNL:
-
standard nutrient liquid
References
Ostwald, W. (1920) Verfahren zum Verdampfen von Flüssigkeiten, Patentschrift Nr. 327976, Klasse 12a, Gruppe 2, Reichspatentamt.
Britten M., and Lavoie, L. (1992) Foaming Properties of Proteins as Affected by Concentration, J. Food. Sci. 57, 1219–1222.
Du, L., Loha, V., and Tanner, R.D. (2000) Modeling a Protein Foam Fractionation Process, Appl. Biochem. Biotechnol. 84–86, 1087–1099.
Brown, A.K., Kaul, A., and Varley, J. (1999) Continuous Foaming for Protein Recovery: Part I. Recovery of β-Casein, Biotechnol. Bioeng. 62, 278–290.
Parlar, H., Gschwendtner, O., Anschütz, A., Leupold, G., and Görg, A. (2001) Influence of Selected Parameters on the Isoelectric Adsorptive Bubble Separation (IABS) of Potato Proteins, Adv. Food Sci. 23, 2–10.
Schügerl K. (2000) Recovery of Proteins and Microorganism from Cultivation Media by Foam Flotation, Adv. Biochem. Eng. Biotechnol. 68, 191–233.
Belitz, H.-D., Grosch, W., and Schieberle, P. (2004) Food Chemistry, 3rd rev. edn., pp. 61–62, Springer Verlag, Berlin.
Damodaran, S. (1997) Protein-Stabilized Foams and Emulsions, in Food Proteins and Their Applications (Damodaran, S., and Paraf, A., eds.), pp. 57–110, Marcel Dekker, New York.
Fernandes, S., Mattiasson, B., and Hatti-Kaul, R. (2002) Recovery of Recombinant Cutinase Using Detergent Foam, Biotechnol. Prog. 18, 116–123.
Lockwood, C.E., Bummer, P.M., and Jay, M. (1997) Purification of Proteins Using Foam Fractionation, Pharm. Res. 14, 1511–1515.
Varley, J., Brown, A.K., Boyd, J.W.R., Dodd, P.W., and Gallagher, S. (2004) Dynamic Multi-point Measurement of Foam Behaviour for a Continuous Fermentation over a Range of Key Process Variables, Biochem. Eng. J. 20, 61–72.
Suzuki, A., and Maruyama, H. (2001) Influence of Liquid Properties and Operating Variables on Enrichment Ratio in Nonfoaming and Foaming Adsorptive Bubble Separation Techniques, J. Colloid. Sci. 238, 54–61.
Arreguín-Espinosa, R., Arreguín, B., and Gonzáles, C. (2000) Purification and Properties of a Lipase from Cephaloleia presignis (Coleoptera, Chrysomelidae), Biotechnol. Appl. Biochem. 31, 239–244.
Freire, D.M., Teles, E.M.F., Bon, E.P.S., and Sant'Anna, G.L. (1997) Lipase Production by Penicillium restrictum in a Bench-Scale Fermenter, Appl. Biochem. Biotechnol. 63–65, 409–421.
Zorn, H., Breithaupt, D.E., Takenberg, M., Schwack, W., and Berger, R.G. (2003) Enzymatic Hydrolysis of Carotenoid Esters of Marigold Flowers (Tagetes erecta L.) and Red Paprika (Capsicum annuum L.) by Commercial Lipases and Pleurotus sapidus Extracellular Lipase, Enzyme Microbiol. Technol. 32, 623–628.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem. 193, 265–275.
Schramm, L.L., and Wassmuth, F. (1994) Foams: Basic Principles, in Foams: Fundamentals and Applications in Petroleum Industry (Schramm, L.L., ed.), Adv. Chem. 242, pp. 3–45, American Chemical Society, Washington, DC.
Cumper, C.W.N., and Alexander, A.E. (1950) The Surface Chemistry of Proteins, Trans. Faraday Soc. 46, 235–253.
Betancor, L., López-Gallego, F., Hidalgo, A., Alonso-Morales, N., Fuentes, M., Fernández-Lafuente, R., and Guisán, J. (2004) Prevention of Interfacial Inactivation of Enzymes by Coating the Enzyme Surface with Dextran-Aldehyde, J. Biotechnol. 110, 201–207.
DeSouza, A.H.G., Tanner, R.D., and Effler, W.T. (1991) The Effect of pH and Gas Composition on the Bubble Fractionation of Proteins, Appl. Biochem. Biotechnol. 28/29, 655–666.
Charm, S.E., Morningstar, J., Matteo, C.C., and Paltiel, B. (1966) The Separation and Purification of Enzymes Through Foaming, Anal. Biochem. 15, 498–508.
Noel, J., Prokop, A., and Tanner, R.D. (2002) Foam Fractionation of a Dilute Solution of Bovine Lactoferrin, Appl. Biochem. Biotechnol. 98–100, 395–402.
Liu, Z., Liu, Z., Wang, D., Ding, F., and Yuan, N. (1998) On the Denaturation of Enzymes in the Process of Foam Fractionation, Bioseparation 7, 167–174.
Burapatana, V., Prokop, A., and Tanner, R.D. (2004) Degeneration of β-Glucosidase Activity in a Foam Fractionation Process, Appl. Biochem. Biotechnol. 113–116, 619–625.
Linko, Y.-Y., and Wu, X.Y. (1996) Biocatalytic Production of Useful Esters by Two Forms of Lipase from Candida, rugosa, J. Chem. Tech. Biotechnol. 65, 163–170.
Yadav, R.P., Saxena, R.K., Gupta, R., and Davidson, W.S. (1998) Purification and Characterization of a Regiospecific Lipase from Aspergillus terreus, Biotechnol. Appl. Biochem. 28, 243–249.
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Linke, D., Zorn, H., Gerken, B. et al. Foam fractionation of exo-lipases from a growing fungus (Pleurotus sapidus). Lipids 40, 323–327 (2005). https://doi.org/10.1007/s11745-005-1389-x
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DOI: https://doi.org/10.1007/s11745-005-1389-x