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Metabolities of dietary triacylglycerol and diacylglycerol during the digestion process in rats

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Lipids

Abstract

The present study investigated the metabolic fate of dietary TAG and DAG and also their digestion products in the stomach and small intestine. A diet containing 10% TAG or DAG oil, enriched in 1,3-DAG, was fed to Wistar rats ad libitum for 9 d. After 18 h of fasting, each diet was re-fed ad libitum for 1 h. The weights of the contents of the stomach and small intestine were measured, and the acylglycerol and FFA levels were analyzed by GC at 0, 1, and 4 h after the 1-h re-feeding. The amounts of re-fed diet ingested and the gastric and small intestinal content were not different between the two diet groups. In the TAG diet group, the main products were TAG and DAG, especially 1(3),2-DAG. In addition, 1,3-DAG and 1(3)-MAG were present in the stomach, and the 1,3-DAG levels increased over time after the re-feeding period. In the DAG diet group, the main products in the stomach were DAG, MAG, FFA, and TAG. There were significantly greater amounts of 1,3-DAG, 1(3)-MAG, and FFA in the DAG diet group in the stomach compared with the TAG diet group. The amount of FFA in the stomach relative to the amount of ingested TAG plus DAG in the DAG diet group was higher than that in the TAG diet group. Acylglycerol and FFA levels were considerably lower in the small intestine than in the stomach. These results indicate that, in the stomach, where acyl migration might occur, the digestion products were already different between TAG and DAG oil ingestion, and that DAG might be more readily digested by lingual lipase compared with TAG. Furthermore, almost all of the dietary lipid was absorbed, irrespective of the structure of the acylglycerol present in the small intestine.

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Abbreviations

HSL:

hormone-sensitive lipase

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Authors and Affiliations

  1. Health Care Products Research Laboratories No. 1, Kao Corporation, 131-8501, Tokyo, Japan

    Noboru Matsuo

  2. Department of Biochemistry, Teikyo University School of Medicine, 173-8605, Tokyo, Japan

    Hiroyuki Shimasaki

  3. Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai, Haga, 321-3497, Tochigi, Japan

    Noriko Osaki, Shinichi Meguro, Noriyuki Yajima & Ichiro Tokimitsu

Authors
  1. Noriko Osaki
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  2. Shinichi Meguro
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  3. Noriyuki Yajima
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  4. Noboru Matsuo
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  5. Ichiro Tokimitsu
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  6. Hiroyuki Shimasaki
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Osaki, N., Meguro, S., Yajima, N. et al. Metabolities of dietary triacylglycerol and diacylglycerol during the digestion process in rats. Lipids 40, 281–286 (2005). https://doi.org/10.1007/s11745-005-1383-3

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  • DOI: https://doi.org/10.1007/s11745-005-1383-3

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