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A study on the causes for the elevated n−3 fatty acids in cows' milk of alpine origin

Lipids volume 40, pages 191–202 (2005)Cite this article

Abstract

The influence of grass-only diets either from rye-grass-dominated lowland pastures (400 m above sea level) or botanically diverse alpine pastures (2000 m) on the FA profile of milk was investigated using three groups of six Brown Swiss cows each. Two groups were fed grass-only on pasture (P) or freshly harvested in barn (B), both for two experimental periods in the lowlands and, consecutively, two periods on the alp. Group C served as the control, receiving a silage-concentrate diet and permanently staying in the lowlands. Effects of vegetation stage or pasture vs. barn feeding on milk fat composition were negligible. Compared with the control, α-linoleic acid (18∶3n−3) consumption was elevated in groups P and B (79%, P<0.001) during the lowland periods but decreased on the alp to the level of C owing to feed intake depression and lower 18∶3n−3 concentration in the alpine forage. Average 18∶3n−3 contents of milk fat were higher in groups, P and B than in C by 33% (P<0.01) at low and by 96% (P<0.001) at high altitude, indicating that 18∶3n−3 levels in milk were to some extent independent of 18∶3n−3 consumption. The cis-9,trans-11 CLA content in milk of grass-fed cows was higher compared with C but lower for the alpine vs. lowland periods whereas the trans-11, cis-13 isomer further increased with altitude. Long-chain n−3 FA and phytanic acid increased while arachidonic acid decreased with grass-only feeding, but none of them responded to altitude. Grass-only feeding increased milk α-tocopherol concentration by 86 and 134% at low and high altitude (P<0.001), respectively. Changes in the ruminal ecosystem due to energy shortage or specific secondary plant metabolites are discussed as possible causes for the high 18∶3n−3 concentrations in alpine milk.

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Abbreviations

18∶3n−3:

α-linolenic acid

B:

barn group

BHB:

β-hydroxybutyrate

C:

control group

CRC:

controlled release alkane capsules

DM:

dry matter

LS:

least squares

NEFA:

nonesterified FA

P:

pasture group

SFA:

saturated FA

References

  1. Innocente, N., Praturlon, D., and Corradini, C. (2002) Fatty Acid Profile of Cheese Produced with Milk from Cows Grazing on Mountain Pastures, Ital. J. Food Sci. 3, 217–224.

    Google Scholar 

  2. Zeppa, G., Giordano, M., Gerbi, V., and Arlorio, M. (2003) Fatty Acid Composition of Piedmont “Ossolano” Cheese, Lait 83, 167–173.

    CAS  Article  Google Scholar 

  3. Kraft, J., Collomb, M., Möckel, P., Sieber, R., and Jahreis, G. (2003) Differences in CLA Isomer Distribution of Cow's Milk Lipids, Lipids 38, 657–664.

    PubMed  CAS  Article  Google Scholar 

  4. Hauswirth, C.B., Scheeder, M.R.L., and Beer, J.H. (2004) High Omega-3 Fatty Acid Content in Alpine Cheese—The Basis for an Alpine Paradox, Circulation 109, 103–107.

    PubMed  CAS  Article  Google Scholar 

  5. Bianchi, M., Fortina, R., Battaglini, L., Mimosi, A., Lussiana, C., and Ighina, A. (2003) Characterisation of Milk Production in Some Alpine Valleys of Piemonte, Ital. J. Anim. Sci. 2, 305–307.

    Google Scholar 

  6. Simopoulos, A.P. (2002) The Importance of the Ratio of Omega-3/Omega-6 Essential Fatty Acids, Biomed. Pharmacother. 56, 365–379.

    PubMed  CAS  Article  Google Scholar 

  7. Belury, M.A. (2002) Dietary Conjugated Linoleic Acid in Health: Physiological Effects and Mechanisms of Action, Annu. Rev. Nutr. 22, 505–531.

    PubMed  CAS  Article  Google Scholar 

  8. Nakamura, T., Azuma, A., Kuribayashi, T., Sugihara, H., Okuda, S., and Nakagawa, M. (2003) Serum Fatty Acid Levels, Dietary Style and Coronary Heart Disease in Three Neighbouring Areas in Japan: The Kumihama Study, Br. J. Nutr. 89, 267–272.

    PubMed  CAS  Article  Google Scholar 

  9. Agenäs, S., Holtenius, K., Griinari, M., and Burstedt, E. (2002) Effects of Turnout to Pasture and Dietary Fat Supplementation on Milk Fat Composition and Conjugated Linoleic Acid in Dairy Cows, Acta Agric. Scand. 52A, 25–33.

    Google Scholar 

  10. Wijesundera, C., Shen, Z., Wales, W.J., and Dalley, D.E. (2003) Effect of Cereal Grain and Fibre Supplements on the Fatty Acid Composition of Milk Fat of Grazing Dairy Cows in Early Lactation, J. Dairy Res. 70, 257–265.

    PubMed  CAS  Article  Google Scholar 

  11. Fievez, V., Vlaeminck, B., Raes, K., Chow, T.T., De Smet, S., Demeyer, D., and Bruinenberg, M.H. (2002) Dietary and Milk Fatty Acid Composition in Relation to the Use of Forages from Semi-natural Grasslands, Grassl. Sci. Eur. 7, 558–559.

    Google Scholar 

  12. Ferlay, A., Martin, B., Pradel, P., Capitan, P., Coulon, J.B., and Chilliard, Y. (2002) Effect of the Nature of Forages on Cow Milk Fatty Acids Having a Positive Role on Human Health, Grassl. Sci. Eur. 7, 556–557.

    Google Scholar 

  13. Chilliard, Y., Ferlay, A., and Doreau, M. (2001) Effect of Different Types of Forages, Animal Fat or Marine Oils in Cow's Diet on Milk Fat Secretion and Composition, Especially Conjugated Linoleic Acid (CLA) and Polyunsaturated Fatty Acids, Livest. Prod. Sci. 70, 31–48.

    Article  Google Scholar 

  14. Boufaied, H., Chouinard, P.Y., Tremblay, G.F., Petit, H.V., Michaud, R., and Belanger, G. (2003) Fatty Acids in Forages. II. In vitro Ruminal Biohydrogenation of Linolenic and Linoleic Acids from Timothy, Can. J. Anim. Sci. 83, 513–522.

    CAS  Google Scholar 

  15. Dewhurst, R.J., Moorby, J.M., Scollan, N.D., Tweed, J.K.S., and Humphreys, M.O. (2002) Effects of a Stay-Green Trait on the Concentrations and Stability of Fatty Acids in Perennial Ryegrass, Grass Forage Sci. 57, 360–366.

    CAS  Article  Google Scholar 

  16. Collomb, M., Bütikofer, U., Sieber, R., Jeangros, B., and Bosset, J.-O. (2002) Correlation Between Fatty Acids in Cows' Milk Fat Produced in the Lowlands, Mountains and Highlands of Switzerland and Botanical Composition of the Fodder, Int. Dairy J. 12, 661–666.

    CAS  Article  Google Scholar 

  17. Martin, B., Fedele, V., Ferlay, A., Grolier, P., Rock, E., Gruffat, D., and Chilliard, Y. (2004) Effects of Grass-Based Diets on the Content of Micronutrients and Fatty Acids in Bovine and Caprine Dairy Products, Grassl. Sci. Eur. 9, 876–886.

    Google Scholar 

  18. Berry, N.R., Scheeder, M.R.L., Sutter, F., Kröber, T.F., and Kreuzer, M. (2000) The Accuracy of Intake Estimation Based on the Use of Alkane Controlled-Release Capsules and Faeces Grab Sampling in Cows, Ann. Zootech. 49, 3–13.

    CAS  Article  Google Scholar 

  19. Braun-Blanquet, J. (1964) Pflanzensoziologie, 3rd edn., Springer Verlag, Vienna.

    Google Scholar 

  20. Suter, B., Grob, K., and Pacciarelli, B. (1997) Determination of Fat Content and Fatty Acid Composition Through 1-min Transesterification in the Food Sample; Principles, Z. Lebensm. Unters. Forsch. A 204, 252–258.

    CAS  Article  Google Scholar 

  21. Collomb, M., and Bühler, T. (2000) Analyse de la composition en acides gras de la graisse de lait, Mitt. Lebensm. Hyg. 91, 306–332.

    Google Scholar 

  22. Wolff, R.L., and Precht, D. (2002) A Critique of 50-m CP-Sil 88 Capillary Columns Used Alone to Assess trans-Unsaturated Fatty Acids in Foods: The Case of the TRANSFAIR Study, Lipids 37, 627–629.

    PubMed  CAS  Article  Google Scholar 

  23. Kramer, J.K.G., Blackadar, C.B., and Zhou, J. (2002) Evaluation of Two GC Columns (60-m SUPELCOWAX 10 and 100-m CP Sil 88) for Analysis of Milkfat with Emphasis on CLA, 18∶1, 18∶2 and 18∶3 Isomers, and Short- and Long-Chain FA, Lipids 37, 823–835.

    PubMed  CAS  Article  Google Scholar 

  24. Collomb, M., Sieber, R., and Bütikofer, U. (2004) CLA Isomers in Milk Fat from Cows Fed Diets with High Levels of Unsaturated Fatty Acids, Lipids 39, 355–364.

    PubMed  CAS  Google Scholar 

  25. Wettstein, H.-R., Scheeder, M.R.L., Sutter, F., and Kreuzer, M. (2001) Effect of Lecithins Partly Replacing Rumen-Protected Fat on Fatty Acid Digestion and Composition of Cow Milk, Eur. J. Lipid Sci. Technol. 103, 12–22.

    CAS  Article  Google Scholar 

  26. Rettenmaier, R., and Schüep, W. (1992) Determination of Vitamins A and E in Liver Tissue, Int. J. Vitam. Nutr. Res. 62, 312–317.

    PubMed  CAS  Google Scholar 

  27. Leiber, F., Kreuzer, M., Jörg, B., Leuenberger, H., and Wettstein, H.-R. (2004) Contribution of Altitude and Alpine Origin of Forage to the Influence of Alpine Sojourn of Cows on Intake, Nitrogen Conversion, Metabolic Stress and Milk Synthesis, Anim. Sci. 78, 451–466.

    Google Scholar 

  28. RAP (Station federale de recherches en production animále) (1999) Fütterungsempfehlungen und Nährwerttabellen für Wiederkäuer, 4th edn. Landwirtschaftliche Lehrmittelzentrale, Zollikofen, Switzerland.

    Google Scholar 

  29. Wachira, A.M., Sinclair, L.A., Wilkinson, R.G., Hallett, K., Enser, M., and Wood, J.D. (2000) Rumen Biohydrogenation of n−3 Polyunsaturated Fatty Acids and Their Effects on Microbial Efficiency and Nutrient Digestibility in Sheep, J. Agric. Sci. 135, 419–428.

    CAS  Article  Google Scholar 

  30. Sasaki, H., Horiguchi, K., and Takahashi, T. (2001) Effects of Different Concentrate and Roughage Ratios on Ruminal Balance of Long-Chain Fatty Acids in Sheep, Asian-Austral. J. Anim. Sci. 14, 960–965.

    CAS  Google Scholar 

  31. Song, M.K. (2000) Fatty Acid Metabolism by Rumen Microorganisms, Asian-Austral. J. Anim. Sci. 13, 137–148.

    CAS  Google Scholar 

  32. Griinari, J.M., Corl, B.A., Lacy, S.H., Chouinard, P.Y., Nurmela, K.V.V., and Bauman, D.E. (2000) Conjugated Linoleic Acid Is Synthesized Endogenously in Lactating Dairy Cows by Δ9-Desaturase, J. Nutr. 130, 2285–2291.

    PubMed  CAS  Google Scholar 

  33. Kelly, M.L., Kolver, E.S., Baumann, D.E., van Amburgh, M.E., and Muller, L.D. (1998) Effect of Intake of Pasture on Concentrations of Conjugated Linoleic Acid in Milk of Lactating Cows, J. Dairy Sci. 81, 1630–1636.

    PubMed  CAS  Article  Google Scholar 

  34. Elgersma, A., Ellen, G., and Tamminga, S. (2004) Rapid Decline of Contents of Beneficial Omega-7 Fatty Acids in Milk from Grazing Cows with Decreasing Herbage Allowance, Grassl. Sci. Eur. 9, 1136–1138.

    Google Scholar 

  35. Sinclair, A.J., Attar-Bashi, N.M., and Li, D. (2002) What Is the Role of α-Linolenic Acid for Mammals? Lipids 37, 1113–1123.

    PubMed  CAS  Google Scholar 

  36. Soppela, P., and Nieminen, M. (2002) Effect of Moderate Wintertime Undernutrition on Fatty Acid Composition of Adipose Tissues of Reindeer (Rangifer tarandus tarandus L.), Comp. Biochem. Physiol. A 132, 403–409.

    Article  Google Scholar 

  37. Boufaied, H., Chouinard, P.Y., Tremblay, G.F., Petit, H.V., Michaud, R., and Belanger, G. (2003) Fatty Acids in Forages. I. Factors Affecting Concentrations, Can. J. Anim. Sci. 83, 501–511.

    CAS  Google Scholar 

  38. Christen, R.E., Kunz, P.L., Langhans, W., Leuenberger, H., Sutter, F., and Kreuzer, M. (1996) Productivity, Requirements and Efficiency of Feed and Nitrogen Utilization of Grass-fed Early Lactating Cows Exposed to High Alpine Conditions, J. Anim. Physiol. Anim. Nutr. 76, 22–35.

    CAS  Article  Google Scholar 

  39. Kreuzer, M., Langhans, W., Sutter, F., Christen, R.E., Leuenberger, H., and Kunz, P.L. (1998) Metabolic Response of Early Lactating Cows Exposed to Transport and High Altitude Grazing Conditions, Anim. Sci. 67, 237–248.

    Article  Google Scholar 

  40. Barry, T.N., and McNabb, W.C. (1999) The Implications of Condensed Tannins on the Nutritive Value of Temperature Forages Fed to Ruminants, Br. J. Nutr. 81, 263–272.

    PubMed  CAS  Google Scholar 

  41. Mariaca, R.G., Berger, T.F.H., Gauch, R., Imhof, M.I., Jeangros, B., and Bosset, J.O. (1997) Occurrence of Volatile Mono- and Sesquiterpenoids in Highland and Lowland Plant Species as Possible Precursors for Flavor Compounds in Milk and Dairy Products, J. Agric. Food Chem. 45, 4423–4434.

    CAS  Article  Google Scholar 

  42. Min, B.R., Barry, T.N., Attwood, G.T., and McNabb, W.C. (2003) The Effect of Condensed Tannins on the Nutrition and Health of Ruminants Fed Fresh Temperate Forages: A Review, Anim. Feed Sci. Technol. 106, 3–19.

    CAS  Article  Google Scholar 

  43. Dewhurst, R.J., Evans, R.T., Scollan, N.D., Moorby, J.M., Merry, R.J., and Wilkins, R.J. (2003) Comparison of Grass and Legume Silages for Milk Production. 2. In vivo and in sacco Evaluations of Rumen Function, J. Dairy Sci. 86, 2612–2621.

    PubMed  CAS  Google Scholar 

  44. Offer, N.W., Mardsen, M., Dixon, J., Speake, B.K., and Thacker, F.E. (1999) Effect of Dietary Fat Supplements on Levels of n−3 Poly-unsaturated Fatty Acids, trans Acids and Conjugated Linoleic Acid in Bovine Milk, Anim. Sci. 69, 613–625.

    CAS  Google Scholar 

  45. Brown, P.J., Mei, G., Gibberd, F.B., Burston, D., Mayne, P.D., McClinchy, J.E., and Sidey, M. (1993) Diet and Refsum's Disease. The Determination of Phytanic Acid and Phytol in Certain Foods and the Application of This Knowledge to the Choice of Suitable Convenience Foods for Patients with Refsum's Disease, J. Hum. Nutr. Diet. 6, 295–305.

    Google Scholar 

  46. McCarty, M.F. (2001) The Chlorophyll, Metabolite Phytanic Acid Is a Natural Rexinoid—Potential for Treatment and Prevention of Diabetes, Med. Hypoth. 56, 217–219.

    CAS  Article  Google Scholar 

  47. Mukherji, M., Schofield, C.J., Wierzbicki, A.S., Jansen, G.A., Wanders, R.J.A., and Lloyd, M.D. (2003) The Chemical Biology of Branched-chain Lipid Metabolism, Prog. Lipid Res. 42, 359–376.

    PubMed  CAS  Article  Google Scholar 

  48. Al-Mabruk, R.M., Beck, M.F.G., and Dewhurst, R.J. (2004) Effects of Silage Species and Supplemental Vitamin E on the Oxidative Stability of Milk, J. Dairy Sci. 87, 406–412.

    PubMed  CAS  Article  Google Scholar 

  49. Jensen, S.K., Johannsen, A.K.B., and Hermansen, J.E. (1999) Quantitative Secretion and Maximal Secretion Capacity of Retinol, β-Carotene and α-Tocopherol into Cows' Milk, J. Dairy Res. 66, 511–522.

    PubMed  CAS  Article  Google Scholar 

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Authors and Affiliations

  1. Institute of Animal Science, Swiss Federal Institute of Technology (ETH), CH-8092, Zurich, Switzerland

    Florian Leiber, Michael Kreuzer, Daniel Nigg, Hans-Rudolf Wettstein & Martin Richard Leo Scheeder

Authors
  1. Florian Leiber
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  2. Michael Kreuzer
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  3. Daniel Nigg
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  4. Hans-Rudolf Wettstein
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  5. Martin Richard Leo Scheeder
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Correspondence to Michael Kreuzer.

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Leiber, F., Kreuzer, M., Nigg, D. et al. A study on the causes for the elevated n−3 fatty acids in cows' milk of alpine origin. Lipids 40, 191–202 (2005). https://doi.org/10.1007/s11745-005-1375-3

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  • DOI: https://doi.org/10.1007/s11745-005-1375-3

Keywords

  • Phytanic Acid
  • Milk Yield
  • Alpine Pasture
  • Pasture Group
  • Total Fame