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Phosphatidylcholine hydroperoxide levels in human plasma are lower than previously reported

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Lipids

Abstract

The quantification of PC hydroperoxide (PCOOH) in human plasma was studied by HPLC with chemiluminescence detection (HPLC-CL). We identified for the first time the monohydroperoxide of 1-palmitoyl-2-linoleoyl-PC hydroperoxide (PC 16∶0/18∶2-OOH) in plasma by LC-MS and HPLC-CL. The standard compound, PC 16∶0/18∶2-OOH (synthetic PCOOH), as well as PCOOH from egg yolk, was used. Comparison of the PCOOH concentration in each participant's plasma as determined by use of a Finepak SIL NH2 column with 2-propanol/methanol/water as the mobile phase (system A, the conventional method) gave a higher concentration than did an LC-18-DB column with methanol containing 0.01% triethylamine (system B). The mean PCOOH concentration for the 43 healthy volunteers was 55.1±30.4 pmol/mL (mean±SD) for system A and 16.3±9.9 pmol/mL for system B. Moreover, the main peak of the plasma extract appeared at a different time from that of synthetic PCOOH or egg yolk PCOOH in system A, whereas in system B plasma sample retention time practically corresponded to that of standard PCOOH. These findings confirm that the PCOOH plasma concentration is not so high as previously reported.

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Abbreviations

BMI:

body mass index

CL:

chemiluminescence detection

ESI:

electrospray ionization

PCOOH:

PC hydroperoxide

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Authors and Affiliations

  1. Kobe Pharmaceutical University, Kobe, Japan

    Takaeki Naito

  2. Department of Legal Medicine, Kobe University Graduate School of Medicine, 7 Kusunoki-cho, Chuo-ku, 650-0017, Kobe, Japan

    Junko Adachi, Naoki Yoshioka, Rika Funae, Yasushi Nagasaki & Yasuhiro Ueno

Authors
  1. Junko Adachi
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  2. Naoki Yoshioka
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  3. Rika Funae
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  4. Yasushi Nagasaki
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  5. Takaeki Naito
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  6. Yasuhiro Ueno
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Correspondence to Junko Adachi.

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Adachi, J., Yoshioka, N., Funae, R. et al. Phosphatidylcholine hydroperoxide levels in human plasma are lower than previously reported. Lipids 39, 891–896 (2004). https://doi.org/10.1007/s11745-004-1311-6

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  • DOI: https://doi.org/10.1007/s11745-004-1311-6

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