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Successful utilization of lyophilized lipoprotein(a) as a biological reagent

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Lipids

Abstract

Lipoprotein(a) [Lp(a)] represents a class of lipoprotein particles having as a protein moiety apoB-100 linked by a single disulfide bond to apolipoprotein(a) [apo(a)], a multikringle structure with a high degree of homology with plasminogen. A recognized feature of Lp(a) is its instability on storage caused by attendant protein and lipid modifications that affect the structural, functional, and immunological properties of this lipoprotein. Here we present data showing that, under appropriate conditions of cryopreservation, Lp(a) retains the properties of the freshly isolated product, and we provide examples supporting the stability of this cryopreserved product as a primary standard in immunoassay settings and in cell culture systems.

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Abbreviations

APMSF:

(4-amidinophenyl)-methanesulfonyl fluoride

apo(a):

apolipoprotein(a)

apoB-100:

apolipoprotein B-100

G3PDH:

glyceraldehyde-3-phosphate-dehydrogenase

KI:

kallikrein inactivator

IL-8:

interleukin 8

Lp(a):

lipoprotein(a)

lyo-Lp(a):

lyophilized Lp(a)

ox:

oxidized

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Chicago, 60637, Chicago, Illinois

    Angelo M. Scanu

  2. Department of Medicine MC5041, University of Chicago, 5841 S. Maryland Ave., 60637, Chicago, IL

    Angelo M. Scanu, Janet Hinman, Ditta Pfaffinger & Celina Edelstein

Authors
  1. Angelo M. Scanu
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  2. Janet Hinman
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  3. Ditta Pfaffinger
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  4. Celina Edelstein
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Correspondence to Angelo M. Scanu.

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Scanu, A.M., Hinman, J., Pfaffinger, D. et al. Successful utilization of lyophilized lipoprotein(a) as a biological reagent. Lipids 39, 589–593 (2004). https://doi.org/10.1007/s11745-004-1268-5

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  • DOI: https://doi.org/10.1007/s11745-004-1268-5

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