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Influences of dietary n−3 long-chain PUFA on body concentrations of 20∶5n−3, 22∶5n−3, and 22∶6n−3 in the larvae of a marine teleost fish from Australian waters, the striped trumpeter (Latris lineata)


We determined the effect of dietary long-chain (≥C20) PUFA (LC-PUFA), 20∶5n−3 and 22∶6n−3, on larval striped trumpeter (Latris lineata) biochemistry through early development and during live feeding with rotifers (Brachionus plicatilis). Rotifers were enriched using seven experimental emulsions formulated with increasing concentrations of n−3 LC-PUFA, mainly 20∶5n−3 and 22∶6n−3. Enriched rotifer n−3 LC-PUFA concentrations ranged from 10–30 mg/g dry matter. Enriched rotifers were fed to striped trumpeter larvae from 5 to 18 d post-hatch (dph) in a short-term experiment to minimize gross deficiency symptoms such as poor survival that could confound results. No relationships were observed between larval growth or survival with dietary n−3 LC-PUFA at 18 dph. The larval FA profiles generally reflected those of the rotifer diet, and significant positive regressions were observed between most dietary and larval FA at 10, 14, and 18 dph. The major exception observed was an inverse relationship between dietary and larval 22∶5n−3. The presence of 22∶5n−3 in elevated amounts when dietary 22∶5n−3. The presence of 22∶5n−3 in elevated amounts when dietary 22∶6n−3 was depressed suggests that elongation of 20∶5n−3 may be occurring in an attempt to raise body concentrations of 22∶6n−3. We hypothesize that accumulation of 22∶5n−3 might be an early indicator of 22∶6n−3 deficiency in larval fish that precedes a reduction in growth or survival. A possible role of 22∶5n−3 as a biochemical surrogate for 22∶6n−3 is discussed.

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dry matter


days post-hatch


monounsaturated FA


long-chain PUFA


saturated FA


total FA


tuna oil


  1. 1.

    Sargent, J.R., Tocher, D.R., and Bell, J.G. (2002) The Lipids, in Fish Nutrition (Halver, J.E., and Hardy, R.W., eds.), pp. 181–257, Academic Press, San Diego.

    Google Scholar 

  2. 2.

    Tocher, D.R. (2003) Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish, Rev. Fish. Sci. 2, 107–184.

    Google Scholar 

  3. 3.

    Izquierdo, M.S. (1996) Essential Fatty Acid Requirements of Cultured Marine Fish Larvae, Aquacult. Nutr. 2, 183–191.

    CAS  Article  Google Scholar 

  4. 4.

    Tuncer, H., and Harrell, R.M. (1992) Essential Fatty Acid Nutrition of Larval Striped Bass (Morone saxatilis) and Palmetto Bass (M. saxatilis×M. chrysops), Aquaculture 101, 105–121.

    CAS  Article  Google Scholar 

  5. 5.

    Salhi, M., Izquierdo, M.S., Hernández-Cruz, C.M., González, M., and Fernández-Palacios, H. (1994). Effect of Lipid and n−3 HUFA Levels in Microdiets on Growth, Survival, and Fatty Acid Composition of Larval Gilthead Sea Bream (Sparus aurata), Aquaculture 124, 275–282.

    CAS  Article  Google Scholar 

  6. 6.

    Dhert, P., Lavens, P., Duray, M., and Sorgeloos, P. (1990) Improved Larval Survival at Metamorphosis of Asian Seabass (Lates calcarifer) Using ω-3-HUFA-Enriched Live Food, Aquaculture 90, 63–74.

    Article  Google Scholar 

  7. 7.

    Sargent, J.R., Bell, J.G., Bell, M.V., Henderson, R.J., and Tocher, D.R. (1995) Requirement Criteria for Essential Fatty Acids, J. Appl. Ichthyol. 11, 183–198.

    CAS  Google Scholar 

  8. 8.

    Sargent, J., Henderson, R.J., and Tocher, D.R. (1989) The Lipids, in Fish Nutrition (Halver, J.E., ed.), pp. 153–218, Academic Press, San Diego.

    Google Scholar 

  9. 9.

    Bell, M.V., Batty, R.S., Dick, J.R., Fretwell, K., Navarro, J.C., and Sargent, J.R. (1995) Dietary Deficiency of Docosahexaenoic Acid Impairs Vision at Low Light Intensities in Juvenile Herring (Clupea harengus L.), Lipids 30, 443–449.

    PubMed  CAS  Google Scholar 

  10. 10.

    Morehead, D.T., Ritar, A.J., and Pankhurst, N.W. (2000) Effect of Consecutive 9-or 12-Month Photothermal Cycles and Handling on Sex Steroid Levels, Oocyte Development, and Reproductive Performance in Female Striped Trumpeter Latris lineata (Latrididae), Aquaculture 189, 293–305.

    CAS  Article  Google Scholar 

  11. 11.

    Trotter, A.J., Battaglene, S.C., and Pankhurst, P.M. (2003) Effects of Photoperiod and Light Intensity on Initial Swim Bladder Inflation, Growth, and Post-inflation Viability in Cultured Striped Trumpeter (Latris lineata) Larvae, Aquaculture 224, 141–158.

    Article  Google Scholar 

  12. 12.

    Brown, M.R., Skabo, S., and Wilkinson, B. (1998) The Enrichment and Retention of Ascorbic Acid in Rotifers Fed Microalgal Diets, Aquacult. Nutr. 4, 151–156.

    CAS  Article  Google Scholar 

  13. 13.

    Lewis, T., Nichols, P.D., and McMeekin, T.A. (2000) Evaluation of Extraction Methods for Recovery of Fatty Acids from Lipid-Producing Microheterotrophs, J Microbiol. Methods 43, 107–116.

    PubMed  CAS  Article  Google Scholar 

  14. 14.

    Bligh, E.G., and Dyer, W.G. (1959) A Rapid Method of Total Lipid Extraction and Purification, Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  15. 15.

    Koven, W., Barr, Y., Lutzky, S., Ben-Atia, J., Weiss, R., Harel, M., Behrens, P., and Tandler, A. (2001) The Effect of Dietary Arachidonic Acid (20∶4n−6) on Growth, Survival and Resistance to Handling Stress in Gilthead Seabream (Sparus aurata) Larvae, Aquaculture 193, 107–122.

    CAS  Article  Google Scholar 

  16. 16.

    Mourente, G., Rodriguez, A., Tocher, D.R., and Sargent, J.R. (1993) Effects of Dietary Docosahexaenoic Acid (DHA, 22∶6n−3) on Lipid and Fatty Acid Compositions and Growth in Gilthead Sea Bream (Sparus aurata L.) Larvae During First Feeding, Aquaculture 112, 79–98.

    CAS  Article  Google Scholar 

  17. 17.

    Rodriguez, C., Perez, J.A., Izquierdo, M.S., Mora, J., Lorenzo, A., Fernandez-Palacios, H. (1994) Essential Fatty Acid Requirements of Larval Gilthead Sea Bream, Sparus aurata (L.), Aquacult. Fish. Manage. 25, 295–304.

    Google Scholar 

  18. 18.

    Furuita, H., Konishi, K., and Takeuchi, T. (1999) Effect of Different Levels of Eicosapentaenoic Acid and Docosahexaenoic Acid in Artemia nauplii on Growth, Survival, and Salinity Tolerance of Larvae of the Japanese Fluonder, Paralichthys olivaceus, Aquaculture 170, 59–69.

    CAS  Article  Google Scholar 

  19. 19.

    Izquierdo, M.S., Arakawa, T., Takeuchi, T., Haroun, R., and Watanabe, T. (1992) Effect of n−3 HUFA Levels in Artemia on Growth of Larval Japanese Flounder (Paralichthys olivaceus), Aquaculture 105, 73–82.

    CAS  Article  Google Scholar 

  20. 20.

    Harel, M., Lund, E., Gavasso, S., Herbert, R., and Place, A.R. (2000) Modulation of Arachidonate and Docosahexaenoate in Morone chrysops Larval Tissues and the Effect on Growth and Survival, Lipids 35, 1269–1280.

    PubMed  CAS  Article  Google Scholar 

  21. 21.

    Ruyter, B., and Thomassen, M.S. (1999) Metabolism of n−3 and n−6 Fatty Acids in Atlantic Salmon Liver: Stimulation by Essential Fatty Acid Deficiency, Lipids 34, 1167–1176.

    PubMed  CAS  Article  Google Scholar 

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Correspondence to M. P. Bransden.

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Bransden, M.P., Dunstan, G.A., Battaglene, S.C. et al. Influences of dietary n−3 long-chain PUFA on body concentrations of 20∶5n−3, 22∶5n−3, and 22∶6n−3 in the larvae of a marine teleost fish from Australian waters, the striped trumpeter (Latris lineata). Lipids 39, 215–222 (2004).

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  • Live Food
  • Paralichthys Olivaceus
  • Clupea Harengus
  • Cooperative Research Centre
  • Experimental Emulsion