Abstract
Twenty-three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy FA. Two distinct conversion pathways were in operation. Two strains of Lactobacillus acidophilus and a strain of Pediococcus pentosaceus produced 13(S)-hydroxy-9-octadecenoic acid [13(S)-OH 18∶1] and 10,13-dihydroxyoctadecanoic acid (10,13-OH 18∶0) as main and minor products, respectively, whereas 13 strains, including L. casei subsp. casei, L. paracasei subsp, paracasei, L. rhamnosus, L. lactis subsp. cremoris, and Streptococcus salivarius subsp. thermophilus produced 10-hydroxy-12-octadecenoic acid (10-OH 18∶1). Seven strains of L. plantanum converted linoleic acid to 10-hydroxyoctadecanoic acid (10-OH 18∶0) through 10-OH 18∶1 Linoleic acid at 2 g/L was converted by L. acidophilus LFO13951T to 1.3 g of 13(S)-OH 18∶1 and 0.09 g of 10,13-OH 18∶0 in 7 d. Lactobacillus paracasei subsp. paracasei JCM 1111 produced 10-OH 18∶1 in 91% yield, and L. plantarum JCM 8341, 10-OH 18∶0 in 59% yield from linoleic acid (2 g/L) under optimal conditions. To our knowledge, this is the first report on the production of 13(S)-OH 18∶1 by lactic acid bacteria other than ruminal bacteria, and of 10,13-OH 18∶0 by any bacteria.
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Abbreviations
- 10-OH 18∶0:
-
10-hydroxyoctadecanoic acid
- 10-OH 18∶1:
-
10-hydroxy-12-octadecenoic acid
- 10,13-OH 18∶0:
-
10,13-dihydroxyoctadecanoic acid
- 13(S)-OH 18∶1:
-
13(S)-hydroxy-9-octadecenoic acid
- RT:
-
retention time
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Kishimoto, N., Yamamoto, I., Toraishi, K. et al. Two distinct pathways for the formation of hydroxy FA from linoleic acid by lactic acid bacteria. Lipids 38, 1269–1274 (2003). https://doi.org/10.1007/s11745-003-1188-4
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DOI: https://doi.org/10.1007/s11745-003-1188-4