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Genes regulated by arachidonic and oleic acids in Raji cells

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Lipids

Abstract

FA are known to modulate immune function in conditions such as arthritis and lupus erythematosus. The effects of arachidonic (AA) and oleic acids (OA) on function and pleiotropic gene expression of Raji cells were investigated. The following parameters were evaluated: cytotoxicity as assessed by loss of membrane integrity and DNA fragmentation; proliferation as measured by [14C]thymidine incorporation; production of interleukin (IL)-10, interferon (INF)-γ, and tumor necrosis factor (TNF)-α; and expression of pleiotropic genes by a macroarray technique (83 genes in total). AA was more toxic to Raji cells than OA. Both FA promoted an increase in Raji cell proliferation at 75 μM, whereas OA at high concentrations (200 μM) decreased proliferation. AA reduced the production of IL-10, TNF-α, and INF-γ. On the other hand, OA provoked an increase of INF-γ production but did not affect the production of IL-10 and TNF-α. The proportions of genes with altered expression were 27% for AA and 35% for OA. The FA affected the expression of genes clustered as: cytokines, signal transduction pathways, transcription factors, cell cycle, defense and repair, apoptosis, DNA synthesis, cell adhesion, cytoskeleton, and hormone receptors. The most remarkable changes were observed in the genes of signal transduction pathways. These results led us to conclude that the effect of these FA on B-lymphocytes includes regulation of gene expression. Thus, diets enriched with fat containing OA or AA may affect B lymphocyte function in vivo.

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Abbreviations

AA:

arachidonic acid (20∶4n−6)

BAX:

Bcl-2-associated X protein membrane

Bcl-xL:

apoptosis regulator bcl-x

CASP3:

caspase 3

CDK10:

cyclin-dependent kinase 10

CSAID-binding protein:

cytokine-suppressive anti-inflammatory drug-binding protein

Con A:

concanavalin A

IL:

interleukin

HER3:

ErbB3 proto-oncogene

INF:

interferon

JAK-1:

Janus tyrosine-protein kinase 1

MAPKK 1:

3, 6, mitogen-activated protein kinase kinase 1, 3, 6

MAPKp38:

mitogen-activated protein kinase p38

MYBL2:

MYB-related protein B

OA:

oleic acid (18∶1)

PI:

propidium iodide

PKC-β:

protein kinase C-β1

PLA2 :

phospholipase A2

PRKAR2B:

cAMP-dependent protein kinase type II β regulatory subunit

RT-PCR:

reverse transcriptase PCR

SOD1:

cytosolic superoxide dismutase 1

SSC:

0.15 M sodium chloride+0.15 M sodium citrate

TOP2A:

topoisomerase II-α

TNF:

tumor necrosis factor

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Authors and Affiliations

  1. Faculty of Sciences of Health, Physical Education, Methodist University of Piracicaba, São Paulo, Brazil

    Rozangela Verlengia

  2. Department of Biology, University of Ponta Grossa, Paraná, Brazil

    Carla Cristine Kanunfre

  3. Department of Biochemistry, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Ireland

    Philip Newsholme

  4. Departamento de Fisiologia e Biofísica, Instituto de Ciências Biomédicas I, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1524, 05508-900, Butantã, São Paulo, SP, Brasil

    Renata Gorjão, Silvana Bordin, Thais Martins de Lima & Rui Curi

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  1. Rozangela Verlengia
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Correspondence to Rui Curi.

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Verlengia, R., Gorjão, R., Kanunfre, C.C. et al. Genes regulated by arachidonic and oleic acids in Raji cells. Lipids 38, 1157–1165 (2003). https://doi.org/10.1007/s11745-003-1174-x

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  • DOI: https://doi.org/10.1007/s11745-003-1174-x

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