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Hyperphagia modifies FA profiles of plasma phospholipids, plasma FFA, and adipose tissue TAG

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Lipids

Abstract

Hyperphagia was achieved by continuous intracerebroventricular infusion of a melanocortin receptor antagonist (HS024; Neosystem, Strasbourg, France) in rats. The effects of hyperphagia on FA composition and concentration of plasma phospholipids (PL), plasma FFA, and adipose tissue TAG were studied in rats for 8 d [short-term hyperphagia (STH); n=8], or 28 d [longterm hyperphagia (LTH); n=9]. The control rats were treated with artificial cerebrospinal fluid for 8 d (n=8) or 28 d (n=10). The rats were fed the same regular diet. In STH rats the plasma PL and fasting plasma FFA contained higher concentrations of saturated FA (SFA) and monounsaturated FA (MUFA), and plasma FFA contained lower n−6 PUFA than in the control rats. In LTH rats the plasma PL contained higher concentrations of SFA, MUFA, and n−3 PUFA and higher proportions of 16∶1n−7 and 18∶1n−9 at the expense of 18∶2n−6 than in the control rats. In LTH rats the abundant dietary intake of 18∶2n−6 did not enrich 18∶2n−6 of the plasma PL or adipose tissue TAG. In LTH rats the fasting plasma FFA contained more than twofold higher concentrations of SFA and MUFA, and higher proportions of 16∶1n−7 and 18∶1n−9 at the expense of 18∶2n−6 than in the control rats. This animal obesity model shows that LTH affects the FA composition and concentration of plasma PL, plasma FFA, and adipose tissue TAG, a result consistent with changes associated with increased risk of various diseases in humans. These results also demonstrate that LTH alters the FA composition of plasma PL and adipose tissue TAG in a way that does not reflect the FA composition of dietary fat.

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Abbreviations

ACSF:

artificial cerebrosplinal fluid

i.c.v.:

intracerebroventricular

LTH:

long-term hyperphagia

MC:

melanocortin

MUFA:

total monounsaturated FA

PL:

phospholipid

SFA:

total saturated FA

STH:

short-term hyperphagia

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Landspitali University Hospital, Reykjavik, Iceland

    Leifur Franzson

  2. Department of Neuroscience, Uppsala Biomedicinska Centrum, Uppsala University, Uppsala, Sweden

    Helgi B. Schiöth

  3. Department of Physiology, University of Iceland, Vatnsmyrarvegi 16, IS-101, Reykjavik, Iceland

    Gudrun V. Skuladottir, Pall I. Olason, Logi Jonsson, Jon O. Skarphedinsson, Vedis H. Eriksdottir & Palmi Th. Atlason

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  1. Gudrun V. Skuladottir
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  2. Pall I. Olason
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  3. Logi Jonsson
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  4. Jon O. Skarphedinsson
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Correspondence to Gudrun V. Skuladottir.

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Skuladottir, G.V., Olason, P.I., Jonsson, L. et al. Hyperphagia modifies FA profiles of plasma phospholipids, plasma FFA, and adipose tissue TAG. Lipids 38, 1127–1132 (2003). https://doi.org/10.1007/s11745-003-1170-1

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  • DOI: https://doi.org/10.1007/s11745-003-1170-1

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