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Digestion and assimilation features of dietary DAG in the rat small intestine

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Lipids

Abstract

Several recent studies have demonstrated that dietary DAG oil rich in 1,3-species suppresses the postprandial increase of serum TAG level and decreases body fat accumulation, compared with TAG oil. To clarify the mechanisms underlying the beneficial effects of DAG, we investigated the metabolic features of DAG in the small intestine with regard to the digestion pathway in the lumen and the TAG-synthesis pathway in the mucosa. When intraduodenally infused as an emulsion, TAG was digested to 1,2-DAG, 2-MAG, and FFA, whereas 1,3-DAG was digested to 1(3)-MAG and FFA. When assessed by the incorporation of [1-14C]linoleic acid in lipids, the mucosal TAG-synthesis was significantly reduced by DAG infusion compared with TAG infusion. However, the mucosal 1,3-DAG synthesis was remarkably increased in the DAG-infused rats. The total amount of mucosal 1,3-DAG was also increased (4.5-fold) after DAG infusion compared with that after TAG infusion. Next, we examined the synthesis pathway of 1,3-DAG. In cultures of the everted intestinal sacs, 1,3-DAG production required the presence of 1-MAG, suggesting that the 1,3-DAG synthesis was due to acylation of 1(3)-MAG in the DAG-infused rats. Furthermore, measurements of DAG acyltransferase activity indicated that 1,3-DAG was little utilized in TAG synthesis. These findings suggest that features of 1,3-DAG digestion and assimilation in the intestine may be responsible for the reduction of the postprandial serum TAG level by dietary DAG.

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Abbreviations

DGAT:

diacylglycerol acyltransferase

DO:

dioleoylglycerol

IOD:

integrated optical density

MO:

monooleoylglycerol

TO:

trioleoylglycerol

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Authors and Affiliations

  1. Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, 321-3497, Tochigi, Japan

    Hidehiko Kondo, Tadashi Hase, Takatoshi Murase & Ichiro Tokimitsu

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  1. Hidehiko Kondo
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  2. Tadashi Hase
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  3. Takatoshi Murase
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  4. Ichiro Tokimitsu
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Correspondence to Ichiro Tokimitsu.

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Kondo, H., Hase, T., Murase, T. et al. Digestion and assimilation features of dietary DAG in the rat small intestine. Lipids 38, 25–30 (2003). https://doi.org/10.1007/s11745-003-1027-7

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  • DOI: https://doi.org/10.1007/s11745-003-1027-7

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