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Relationship between platelet phospholipid FA and mean platelet volume in healthy men

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Lipids

Abstract

Increased mean platelet volume (MPV) has been suggested as an independent risk factor for acute myocardial infarction and the increased reactivity of large platelets. The aim of this study was to investigate the correlation between platelet phospholipid (PL) PUFA composition and MPV in 139 free-living healthy men ages 20–55 yr (vegans, n=18; ovolacto vegetarians, n=43; modrerate meat-eaters, n=60; and high meateaters, n=18). Each subject completed a semiquantitative Food Frequency Questionnaire and gave a blood sample. Platelet PL FA composition and MPV were determined by standard methods. MPV was significantly greater in the vegans than in the ovolacto vegetarian, moderate, or high meat-eater groups (P<0.01). Both vegan and ovolacto vegetarian groups had significantly higher platelet PL 18∶2n−6 and 22∶4n−6, and lower 20∶5n−3 and 22∶6n−3 compared with the moderate and high meat-eater groups. The vegans demonstrated a significant reduction in 20∶4n−6 and 22∶5n−3 compared with the ovolacto vegetarian, high meat-eater, and moderate meat-eater groups. Bivariate analysis results showed that MPV was significanly positively correlated with platelet PL 18∶2n−6 (P=0.048) and negatively correlated with 20∶3n−6 (P=0.02), 20∶5n−3 (P=0.005), and 22∶5n−3 (P<0.0001), respectively. In a multiple linear regression analysis, after controlling for potential confounding factors such as dietary group, age exercise, body mass index, and dietary polyunsaturated and saturated fat, cholesterol, carbohydrate, and fiber intake, the MPV was still strongly negatively correlated with platelet PL 20∶3n−6 (P=0.003) and 22∶5n−3 (P=0.001). The present data suggest that 20∶5n−3 and 20∶3n−6 may play a role in the structural function of the platelet membrane.

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Abbreviations

BMI:

body mass index

CTAD:

citrate, theophylline, adenosine, and dipyridamole

CVD:

cardiovascular disease

FFQ:

Food Frequency Questionnaire

12-HETE:

12-hydroxy-5,8,10,14-eicosatetraenoic acid

MI:

myocardial infarction

MPV:

mean platelet volume

MUFA:

monounsaturated fatty acid

PG:

prostaglandin

PL:

phospholipid

PRP:

platelet-rich plasma

SFA:

saturated fatty acid

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Authors and Affiliations

  1. Department of Food Science, RMIT University, 3001, Melbourne, Australia

    Duo Li & Andrew J. Sinclair

  2. Department of Medical Laboratory Science, RMIT University, 3001, Melbourne, Australia

    Alan Turner

  3. Department of Food Science, Hangzhou University of Commerce, 310035, Hangzhou, China

    Duo Li

Authors
  1. Duo Li
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  2. Alan Turner
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  3. Andrew J. Sinclair
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Correspondence to Duo Li.

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Li, D., Turner, A. & Sinclair, A.J. Relationship between platelet phospholipid FA and mean platelet volume in healthy men. Lipids 37, 901–906 (2002). https://doi.org/10.1007/s11745-002-0977-0

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  • DOI: https://doi.org/10.1007/s11745-002-0977-0

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